A5037303714- Toxin Mechanisms and Immunotoxins
- Identification and Quantification in Food
- Meat and Animal Product Quality
- Probiotics and Fermented Foods
- Transgenic Plants and Applications
- Biochemical effects in animals
- Osteoarthritis Treatment and Mechanisms
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Proteoglycans and glycosaminoglycans research
- Chromatin Remodeling and Cancer
- Fungal Biology and Applications
- Genomics and Phylogenetic Studies
- Plant tissue culture and regeneration
- Advanced Biosensing Techniques and Applications
- Hemoglobin structure and function
- Fungal and yeast genetics research
- Polyamine Metabolism and Applications
- Silymarin and Mushroom Poisoning
- Veterinary Equine Medical Research
- Herbal Medicine Research Studies
- Macrophage Migration Inhibitory Factor
- Food Safety and Hygiene
- Neonatal Health and Biochemistry
- Genetic Syndromes and Imprinting
University of Campania "Luigi Vanvitelli"
2018-2023
Abstract Background Neuroinflammation contributes to the onset and progression of neurodegenerative diseases, but has not been specifically investigated in patients affected by severe milder forms spinal muscular atrophy (SMA). Methods In this two-center retrospective study, we signatures neuroinflammation forty-eight pediatric male female SMA1 ( n = 18), SMA2 19), SMA3 11) patients, as well a limited number non-neurological control subjects 4). We employed Bio-Plex multiplex system based on...
The pattern recognition receptor long pentraxin-3 (PTX3) plays conflicting roles in cancer by acting as an oncosuppressor or a pro-tumor mediator depending on tumor context. Triple negative breast (TNBC) represents the most aggressive histotype of cancer, characterized lack efficacious therapeutic targets/approaches and poor prognosis. Thus, characterization new molecular pathways and/or alternative druggable targets is great interest TNBC. expression PTX3 BC samples cell lines has been...
Symptomatic slow-acting drugs (SYSADOA) are increasingly used as effective therapies for osteoarthritis, representing an attractive alternative to analgesics or non-steroidal anti-inflammatory relieve disease symptoms. Pharmaceutical preparations of chondroitin sulfate, derived from animal sources, alone in combination with glucosamine widely recognized their beneficial effect on osteoarthritis treatment. A growing interest has also been devoted understanding the molecular mechanisms...
The transcription factor CCCTC-binding (CTCF) modulates pleiotropic functions mostly related to gene expression regulation. role of CTCF in large scale genome organization is also well established. A unifying model explain relationships among many CTCF-mediated activities involves direct or indirect interactions with numerous protein cofactors recruited specific binding sites. co-association other architectural proteins such as cohesin, chromodomain helicases, and BRG1, further supports the...
Quinoin is a type 1 ribosome-inactivating protein (RIP) we previously isolated from the seeds of pseudocereal quinoa (Chenopodium quinoa) and known as functional food for its beneficial effects on human health. As presence RIPs in edible plants could be potentially risky, here further characterised biochemically (complete amino acid sequence, homologies/differences with other three-dimensional homology modeling) explored possible defensive role against pathogens. consists 254 residues,...
Penicillium digitatum is a widespread pathogen responsible for the postharvest decay of citrus, one most economically important crops worldwide. Currently, chemical fungicides are still main strategy to control green mould disease caused by fungus. However, increasing selection and proliferation fungicide-resistant strains require more efforts explore new alternatives acting via or unexplored mechanisms management. To date, several non-chemical compounds have been investigated fungal...
Ribosome-inactivating proteins (RIPs) are a group of with rRNA N-glycosylase activity that catalyze the removal specific adenine located in sarcin–ricin loop large ribosomal RNA, which leads to irreversible inhibition protein synthesis and, consequently, cell death. The case elderberry (Sambucus nigra L.) is unique, since more than 20 RIPs and related lectins have been isolated characterized from flowers, seeds, fruits, bark this plant. However, these kinds never leaves. In work, we purified...
Pharma-grade extractive chondroitin sulfate (CS) is widely used for osteoarthritis (OA) treatment. Recently, unsulfated biofermentative (BC) proved positive effects in OA vitro model. This study, based on primary pathological human synoviocytes, aimed to analyze, by a multiplex assay, panel of OA-related biomarkers response short-term treatments with bovine (CSb), pig (CSp) and fish (CSf) chondroitins, comparison BC. As expected, all samples had anti-inflammatory properties, however CSb, CSf...
Background: The clinical impact of the monoclonal antibody cetuximab targeting EGFR in colorectal cancer (CRC) is widely recognized. Nevertheless, onset resistance a serious issue that limits effectiveness this drug targeted therapies. Unraveling molecular players involved first step towards identification alternative signaling pathways can be to circumvent mechanisms restoring efficacy therapeutic treatments tailored manner. Methods: By applying nanoLC-MS/MS TMT isobaric labeling-based...
Abstract BACKGROUND The Arctic muskox has economic potential as an alternative meat species and is becoming increasingly popular. present study aimed to determine the primary structure pseudoperoxidase activity of myoglobin (Mb) compared cattle water buffalo myoglobins. RESULTS Mb was determined via a matrix‐assisted laser desorption ionization‐time flight mass spectrometry‐based mapping approach using sheep reference sequence. consists 153 amino acid residues shows 100% identity with Mb,...
Background: Monoclonal antibodies (mAbs) against cancer biomarkers are key reagents in diagnosis and therapy. One such relevant biomarker is a preferentially expressed antigen melanoma (PRAME) that selectively many tumors. Knowing mAb’s epitope of utmost importance for understanding the potential activity therapeutic prospective reagents. Methods: We generated mAb PRAME immunizing mice with fragment 161–415; affinity antibody protein was evaluated by ELISA SPR, its ability to detect cells...
Proteins of the MucR/Ros family play a crucial role in bacterial infection or symbiosis with eukaryotic hosts. MucR from Sinorhizobium meliloti plays regulatory establishing host plant, both dependent and independent Quorum Sensing. Here, we report first characterization isolated by mass spectrometry demonstrate that this protein forms higher-order oligomers its native condition expression SEC-MALS. We show purified can bind DNA recognize region upstream ndvA gene EMSA, revealing is direct...
Myoglobin (Mb), oxygen-binding heme-protein retrieved primarily in muscles, is a local oxygen reservoir providing to when delivery insufficient during catabolism. Furthermore, Mb structure studied as example of molecular evolution for its ability respond environmental pressures and adapt it through few amino acid substitutions. Here, order explore the structure–function–dynamics relationships Eurasian woodcock chicken ostrich Mbs we have applied an integrated approach which spectroscopic...
Abstract Background Imprinting Control Regions (ICRs) are CpG-rich sequences acquiring differential methylation in the female and male germline maintaining it a parental origin-specific manner somatic cells. Despite their expected high mutation rate due to spontaneous deamination of methylated cytosines, ICRs show conservation CpG-richness CpG-containing transcription factor binding sites mammalian species. However, little is known about mechanisms contributing maintenance density methyl...