A5079548793- Enzyme Structure and Function
- Protein Structure and Dynamics
- Redox biology and oxidative stress
- Ubiquitin and proteasome pathways
- Endoplasmic Reticulum Stress and Disease
- Bacteriophages and microbial interactions
- Axon Guidance and Neuronal Signaling
- Antimicrobial Peptides and Activities
- Glycosylation and Glycoproteins Research
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Galectins and Cancer Biology
- Computational Drug Discovery Methods
- Antibiotic Resistance in Bacteria
- Photosynthetic Processes and Mechanisms
- Cellular transport and secretion
- Protein Kinase Regulation and GTPase Signaling
- Heat shock proteins research
- Peptidase Inhibition and Analysis
- Biochemical and Structural Characterization
- Chemical Synthesis and Analysis
- Signaling Pathways in Disease
- Toxin Mechanisms and Immunotoxins
- Protein Tyrosine Phosphatases
- Advanced biosensing and bioanalysis techniques
Institute of Biostructure and Bioimaging
2016-2025
Confederazione Nazionale dell'Artigianato e Della Piccola e Media Impresa
2025
National Research Council
2013-2024
University of Naples Federico II
2004-2024
Centro Interuniversitario di Ricerca sui Peptidi Bioattivi
2012-2024
Naples Anesthesia & Physician Associates
2018-2019
Institute of Protein Biochemistry
2005
Sequoia (United States)
2005
Canadian Institutes of Health Research
2005
University of Alberta
2005
Plant extracts are rich in bioactive compounds, such as polyphenols, sesquiterpenes, and triterpenes, which potentially have antiviral activities. As a consequence of the coronavirus disease 2019 pandemic, caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) virus, thousands scientists been working tirelessly trying to understand biology this new virus pathophysiology, with main goal discovering effective preventive treatments therapeutic agents. Plant-derived secondary...
Photodynamic therapy (PDT) may be an excellent alternative in the treatment of breast cancer, mainly for most aggressive type with limited targeted therapies such as triple-negative cancer (TNBC). We recently generated conjugated polymer nanoparticles (CPNs) efficient photosensitizers photo-eradication different cells. With aim improving selectivity PDT CPNs, nanoparticle surface conjugation unique 2’-Fluoropyrimidines-RNA-aptamers that act effective recognition elements functional...
The modelling of peptidoglycan is responsible for key cellular processes in Mycobacterium tuberculosis such as cell growth, division and resuscitation from dormancy. structure M. atypical since it contains a majority 3,3 cross-links synthesized by L,D-transpeptidases that replace the 4,3 formed D,D-transpeptidase activity classical penicillin-binding proteins. Carbapenems inactivate these combination with clavulanic acid are bactericidal against extensively drug-resistant tuberculosis. Here,...
Cationic antimicrobial peptides (AMPs) possess fast and broad-spectrum activity against both Gram-negative Gram-positive bacteria, as well fungi. It has become increasingly evident that many AMPs, including those derive from fragments of host proteins, are multifunctional able to mediate various immunomodulatory functions angiogenesis. Among these, synthetic apolipoprotein-derived safe tolerated in humans have emerged promising candidates the treatment inflammatory conditions. Here, we...
Appetite for ubiquitin: a gushy travel companion in the intracellular journey of amyloid β peptide.
The genus Thermus, which has been considered for a long time as fruitful source of biotechnological relevant enzymes, emerged more recently suitable host to overproduce thermozymes. Among these, α-galactosidases are widely used in several industrial bioprocesses that require high working temperatures and thermostable variants offer considerable advantages over their thermolabile counterparts. Thermus thermophilus HB27 strain was the homologous expression TTP0072 gene encoding an...
Cullin 3 (Cul3) recognition by BTB domains is a key process in protein ubiquitination. Among Cul3 binders, great attention currently devoted to KCTD proteins, which are implicated fundamental biological processes. On the basis of high similarity these it has been suggested that ability bind could be general property among all KCTDs. In order gain new insights into functionality, we here evaluated and/or quantified binding known involved either cullin-independent (KCTD12 and KCTD15) or...
Cystatin B (CSTB) is a ubiquitous protein belonging to superfamily of protease inhibitors. CSTB may play critical role in brain physiology because its mutations cause progressive myoclonic epilepsy-1A (EPM1A), the most common form epilepsy. However, molecular mechanisms underlying Central Nervous System (CNS) are largely unknown. To investigate possible involvement synaptic plasticity, we analyzed expression synaptosomes as model system studying regions CNS. We found that not only present...
Abstract In this paper, we report studies concerning thrombin binding aptamer (TBA) dimeric derivatives in which the 3′-ends of two TBA sequences have been joined by means linkers containing adenosine or thymidine residues and/or a glycerol moiety. CD and electrophoretic investigations indicate that all modified aptamers are able to form G-quadruplex domains resembling parent structure. However, isothermal titration calorimetry measurements aptamer/thrombin interaction point different...
In recent years, the loop-mediated isothermal amplification (LAMP) technique, designed for microbial pathogen detection, has acquired fundamental importance in biomedical field, providing rapid and precise responses. However, it still some drawbacks, mainly due to need a thermostatic block, necessary reach 63 °C, which is BstI DNA polymerase working temperature. Here, we report identification characterization of I Large Fragment from Deinococcus radiodurans (DraLF-PolI) that functions at...
Nm23-H1 is one of the most interesting candidate genes for a relevant role in Neuroblastoma pathogenesis. H-Prune characterized binding partner and its overexpression has been shown different human cancers. Our study focuses on Nm23-H1/h-Prune protein complex Neuroblastoma. Using NMR spectroscopy, we performed conformational analysis h-Prune C-terminal to identify amino acids involved interaction with Nm23-H1. We developed competitive permeable peptide (CPP) impair formation demonstrated...
Cullin3 (Cul3), a key factor of protein ubiquitination, is able to interact with dozens different proteins containing BTB (Bric-a-brac, Tramtrack and Broad Complex) domain. We here targeted the Cul3–BTB interface by using intriguing approach stabilizing α-helical conformation Cul3-based peptides through "stapling" hydrocarbon cross-linker. In particular, combining theoretical experimental techniques, we designed characterized stapled embedding helix 2 (residues 49–68). Intriguingly, CD NMR...
The public health has declared an international state of emergency due to the spread a new coronavirus (SARS-CoV-2) representing real pandemic threat so that find potential therapeutic agents is dire need. To this aim, SARS-CoV-2 spike (S) glycoprotein represents crucial target for vaccines, antibodies, and diagnostics. Since virus binding ACE-2 alone could not be sufficient justify such severe infection, in order facilitate medical countermeasure development search targets, two further...
Article2 May 2021Open Access Source DataTransparent process The TBC1D31/praja2 complex controls primary ciliogenesis through PKA-directed OFD1 ubiquitylation Emanuela Senatore Department of Molecular Medicine and Medical Biotechnologies, University Federico II, Naples, Italy Search for more papers by this author Francesco Chiuso Laura Rinaldi Daniela Intartaglia Telethon Institute Genetics Medicine, Pozzuoli, Rossella Delle Donne Emilia Pedone Biostructures Bioimaging, CNR, Bruno Catalanotti...
Chimera-type galectin-3 (Gal-3) is a β-galactoside-binding protein containing single conserved carbohydrate-recognition domain, crucial in fibrosis and carcinogenesis. Selenium-based Gal-3 inhibitors have emerged as promising therapeutic agents, particularly for treating neoplastic diseases. Among them, seleno-digalactoside (SeDG) substituted with benzyl group at position 3 of both saccharide residues (benzyl 3,3'-seleno-digalactoside, SeDG-Bn), attracted considerable attention its...
Human galectin-3 (Gal-3), a β-galactoside binding lectin through its Carbohydrate Recognition Domain (CRD), is implicated in wide range of cellular functions and involved critical biological processes including pathogen recognition, immune response, inflammation fibrosis. Recently, Gal-3 has gained increasing attention for role pathological conditions such as cancer, where it influences cancer growth progression, inflammatory oxidative stress, opening the search potential inhibitors. In this...
Protein disulfide oxidoreductases are ubiquitous redox enzymes that catalyse dithiol–disulfide exchange reactions with a CXXC sequence motif at their active site. A oxidoreductase, highly thermostable protein, was isolated from Pyrococcus furiosus ( Pf PDO), which is characterized by two sites (CXXC) and an unusual molecular mass. Its 3D structure high resolution suggests it may be related to the multidomain protein disulfide‐isomerase (PDI), currently known only in eukaryotes. This work...