Abstract Background Lactic acid bacteria of the genus Lactobacillus and Bifidobacterium are one most important health promoting groups human intestinal microbiota. Their protective role within gut consists in out competing invading pathogens for ecological niches metabolic substrates. Among features necessary to provide benefits, commensal microorganisms must have ability adhere cells consequently colonize gut. Studies on mechanisms mediating adhesion lactobacilli showed that factors...

Lactococcus lactis is a lactic acid bacterium (LAB), generally recognized as safe, and has been widely used in the food industry, especially fermented dairy products. Numerous studies have evaluated technological probiotic properties of lactococci; however, few reported characteristics L. strains isolated from In this work, potential, including survival simulated gastric juice, tolerance to bile salts, hydrophobicity, auto- co-aggregation, was natural whey starter cultures. The results...

Genomes of gram-positive bacteria encode many putative cell-surface proteins, which the majority has no known function. From rapidly increasing number available genome sequences it become apparent that proteins are conserved, and frequently encoded in gene clusters or operons, suggesting common functions, interactions multiple components. A novel cluster encoding exclusively was identified, is conserved a subgroup bacteria. Each generally one copy four new families called cscA, cscB, cscC...

ABSTRACT The pyruvate oxidase gene ( poxB ) from Lactobacillus plantarum Lp80 was cloned and characterized. Northern blot primer extension analyses revealed that transcription of is monocistronic under the control a vegetative promoter. mRNA expression strongly induced by aeration repressed glucose. Moreover, blotting performed at different stages growth showed maximal in early stationary phase when glucose exhausted. Primer vivo footprint repression mediated CcpA binding to cre site...

ABSTRACT In addition to the previously characterized pyruvate oxidase PoxB, Lactobacillus plantarum genome encodes four predicted oxidases (PoxC, PoxD, PoxE, and PoxF). Each gene was individually inactivated, only knockout of poxF resulted in a decrease activity under tested conditions. We show here that L. has two major oxidases: PoxB PoxF. Both are involved lactate-to-acetate conversion early stationary phase aerobic growth regulated by carbon catabolite repression. A strain devoid...

FtsH proteins have dual chaperone-protease activities and are involved in protein quality control under stress conditions. Although the functional role of has been clearly established, regulatory mechanisms controlling ftsH expression gram-positive bacteria remain largely unknown. Here we show that Lactobacillus plantarum WCFS1 is transiently induced at transcriptional level upon a temperature upshift. In addition, disruption negatively affected growth L. high temperatures. Sequence analysis...

Diclofenac (DCF), a contaminant of emerging concern, is non-steroidal anti-inflammatory drug widely detected in water bodies, which demonstrated harmful acute and chronic toxicity toward algae, zooplankton aquatic invertebrates, therefore its removal from impacted necessary. DCF recalcitrant traditional treatment technologies, thus, innovative approaches are required. Among them, electrochemical oxidation (EO) has shown promising results. In this research, an multidisciplinary approach...

<title>Abstract</title> In the last few years, probiotics have gained much attention within medical, pharmaceutical, and food fields, given health benefits provided by their consumption. They include several lactic acid bacteria (LAB) species, mostly belonging to genera <italic>Lactobacillus</italic>, <italic>Lactococcus</italic>, <italic>Streptococcus</italic>. Postbiotics are bioactive compounds (organic acids, short-chain fatty enzymes, neurotransmitters) produced bacterial fermentation...

ABSTRACT We report the characterization of ccpA gene Lactobacillus plantarum , coding for catabolite control protein A. The is linked to pepQ gene, encoding a proline peptidase, in order ccpA-pepQ with two genes transcribed tandem from same strand as distinct transcriptional units. Two transcription start sites corresponding functional promoters were found, expression upstream promoter being autogenously regulated through catabolite-responsive element ( cre ) sequence overlapping +1 site....

Abstract The aim of this study was to identify genes involved in biofilm development the probiotic lactic acid bacterium Lactobacillus plantarum . ability L. LM3 and some derivative mutant strains form has been investigated. Biofilm microtitre plate assays showed that LM3‐2, carrying a null mutation ccpA gene, coding CcpA master regulator, partially impaired production compared wild type (LM3). Moreover, we found three genome, hereby named flmA , flmB flmC whose deduced amino sequences show...

Collagen is a target of pathogens for adhesion, colonization, and invasion host tissue. Probiotic bacteria can mimic the same mechanism as used by in colonization process, expressing cell surface proteins that specifically interact with extracellular matrix component proteins. The capability to bind collagen expressed several Lactobacillus isolates, including some plantarum strains. In this study we report involvement L. EnoA1 alfa-enolase type I (CnI) binding. By adhesion assays, show...

In southern Italy, some artisanal farms produce mozzarella and caciocavallo cheeses by using natural whey starter (NWS), whose microbial diversity is responsible for the characteristic flavor texture of final product. We studied community NWS cultures cow's milk (NWSc) production buffalo's (NWSb) mozzarella, both from farms. Bacterial identification at species strain level was based on an integrative strategy, combining culture-dependent (sequencing 16S rDNA, species/subspecies-specific...

Lactic acid bacteria (LAB) are widely used in food industry and their growth performance is important for the quality of fermented product. During industrial processes changes temperature may represent an environmental stress to be overcome by starters non-starters LAB. Studies on adaptation heat shock have shown involvement chaperon system-proteins various gram-positive bacteria. The corresponding operons, namely dnaK groESL controlled a negative mechanism involving HrcA repressor protein...

ABSTRACT A newly identified bglH gene coding for a phospho-β-glucosidase of Lactobacillus plantarum was isolated and expressed in Escherichia coli . The sequence analysis the cloned DNA fragment showed an open reading frame encoding 480-amino-acid protein with calculated molecular mass 53 kDa. shown to be on monocistronic transcriptional unit. Its transcription repressed 10-fold L. cells grown glucose compared β-glucoside salicin as sole carbon source. catabolite-responsive element (CRE)...

Mycobacterium tuberculosis has the ability to survive inside macrophages under acid-nitrosative stress. M. Rv1685c and its orthologue in smegmatis, MSMEG_3765, are induced on exposure Both genes annotated as TetR transcriptional regulators, a family of proteins that regulate wide range cellular activities, including multidrug resistance, carbon catabolism virulence. Here we demonstrate MSMEG_3765 is co-transcribed with upstream MSMEG_3762 MSMEG_3763, encoding efflux pump components. RTq-PCR...

Abstract Mesorhizobium loti contains ten genes coding for proteins sharing high amino acid sequence identity with members of the Ros/MucR transcription factor family. Five these family from (Ml proteins) have been recently structurally and functionally characterized demonstrating that Ml are DNA-binding proteins. However, studies were performed using Ros site Currently, there is no evidence as to when expressed during lo ti life cycle well information concerning their natural site. In this...