A5032380606- Enzyme Structure and Function
- Protein Structure and Dynamics
- Gene expression and cancer classification
- Computational Drug Discovery Methods
- Advanced Proteomics Techniques and Applications
- Machine Learning in Bioinformatics
- RNA and protein synthesis mechanisms
- Bioinformatics and Genomic Networks
- Metabolism and Genetic Disorders
- Genetics, Bioinformatics, and Biomedical Research
- Amino Acid Enzymes and Metabolism
- Aquaculture disease management and microbiota
- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Blood properties and coagulation
- Celiac Disease Research and Management
- Plant biochemistry and biosynthesis
- SARS-CoV-2 and COVID-19 Research
- Estrogen and related hormone effects
- Microbial Metabolic Engineering and Bioproduction
- T-cell and B-cell Immunology
- Genomics and Phylogenetic Studies
- Enzyme Production and Characterization
- Biochemical and Molecular Research
- Glycosylation and Glycoproteins Research
Institute of Food Science
2016-2025
National Research Council
2016-2025
University of Zurich
2022
National Academies of Sciences, Engineering, and Medicine
2008-2015
University of Naples Federico II
1993-2010
University of Campania "Luigi Vanvitelli"
1999-2010
Institute of Biomedical Technologies
2010
Centro Interuniversitario di Ricerca sui Peptidi Bioattivi
1998-2008
The identification of gluten peptides eliciting intestinal T cell responses is crucial for the design a peptide-based immunotherapy in celiac disease (CD). To date, several have been identified to be active CD. In present study, we investigated recognition profile immunogenic adult HLA-DQ2(+) patients. Polyclonal, gliadin-reactive lines were generated from jejunal mucosa and assayed both proliferation IFN-gamma production response 21 wheat glutenins alpha-, gamma-, omega-gliadins. A...
Salt bridges can play important roles in protein structure and function have stabilizing destabilizing effects folding.ESBRI is a software available as web tool which analyses the salt structure, starting from atomic coordinates.In case of complexes, between chains be evaluated, well those among specific charged amino acids different subunits, order to obtain useful information regard protein-protein interaction.
Diabetes is a chronic, metabolic disease characterized by high blood sugar levels. Among the main types of diabetes, type 2 most common. Early diagnosis and treatment can prevent or delay onset complications. Previous studies examined application machine learning techniques for prediction pathology, here an artificial neural network shows very promising results as possible valuable aid in management prevention diabetes. Additionally, its superior ability long-term predictions makes it ideal...
Classic galactosemia is a rare disease with long-term consequences that seriously affect the quality of life patients. To date, various therapeutic approaches are being developed, but treatments target molecular defects in mutant galactose-1-phosphate uridylyltransferase (GALT) gene lacking. We conducted computational search for putative pharmacochaperones by applying drug repurposing strategy, and we found one compound, already active as pharmacochaperone another pathology, doubled...
ABSTRACT A methodology based on matrix-assisted laser desorption ionization-time of flight mass spectrometry intact bacterial cells was used for rapid discrimination 24 species, and detailed analyses to identify Escherichia coli O157:H7 were carried out. Highly specific spectrometric profiles pathogenic nonpathogenic bacteria that are well-known major food contaminants obtained, uploaded in a database, made available the Web. In order standardize analytical protocol, several experimental,...
Abstract Background Microarray experiments enable simultaneous measurement of the expression levels virtually all transcripts present in cells, thereby providing a ‘molecular picture’ cell state. On other hand, genomic responses to pharmacological or hormonal stimulus are dynamic molecular processes, where time influences gene activity and expression. The potential use statistical analysis microarray data series has not been fully exploited so far, due fact that only few methods available...
A very large number of computational methods to predict the change in thermodynamic stability proteins due mutations have been developed during last 30 years, and many different web servers are currently available. Nevertheless, most them suffer from severe drawbacks that decrease their general reliability and, consequently, applicability goals such as protein engineering or predictions effects genetic diseases. In this review, we summarized all main approaches used develop these tools, with...
Ulcerative colitis (UC), an inflammatory bowel disease (IBD), may increase the risk of colorectal cancer (CRC) by activating chronic proinflammatory pathways. The goal this study was to find serum prediction biomarkers in UC CRC development combining low-density miRNA microarray and biocomputational approaches. expression profiles were compared microarray, finding five upregulated miRNAs specific progression (hsa-let-7d-5p, hsa-miR-16-5p, hsa-miR-145-5p, hsa-miR-223-5p, hsa-miR-331-3p)....
TP53 encodes p53, which is a nuclear phosphoprotein with cancer-inhibiting properties. In response to DNA damage, p53 activated and mediates set of antiproliferative responses including cell-cycle arrest apoptosis. Mutations in the gene are associated more than 50% human cancers, 90% these affect p53-DNA interactions, resulting partial or complete loss transactivation functions. These mutations structural integrity and/or leading protein's function. We report here results systematic...
Abstract Capsicum is one of the major vegetable crops grown worldwide. Current subdivision in clades and species based on morphological traits coarse sets genetic markers. Broad variability fruits has been driven by breeding programs mainly studied linkage analysis. We discovered 746k variable sites sequencing 1.8% genome a collection 373 accessions belonging to 11 from 51 countries. describe genomic variation at population-level, confirm species, show that known C. annuum separates large...
Histone modifications through acetylation are fundamental for remodelling chromatin and consequently activating gene expression. The imbalance between deacetylation activity causes transcriptional dysregulation associated with several disorders. Flavones, small molecules of plant origin, known to interfere class I histone deacetylase (HDAC) enzymes enhance acetylation, restoring cell homeostasis. To investigate the possible physical interactions flavones on human HDAC1 2, we carried out in...
Abstract Background Despite decades on developing dedicated Web tools, it is still difficult to predict correctly the changes of thermodynamic stability proteins caused by mutations. Here, we assessed reliability five recently developed in order evaluate progresses field. Results The results show that, although there are improvements field, predictors far from ideal. Prevailing problems include bias towards destabilizing mutations, and, general, unreliable when mutation causes a ΔΔG within...
Summary Genic‐cytoplasmic male sterility (CMS) due to interactions between nuclear and cytoplasmic genomes is a well‐known phenomenon in some Solanum spp. hybrids, but genes involved are not known. In this study, the chondriomes of two isonuclear male‐fertile sterile somatic hybrids (SH9A SH9B, respectively) common potato ( S. tuberosum Group Tuberosum , tbr ) wild species commersonii were sequenced compared those parental identify mitochondrial expression sterility. A putative novel gene...
Estrogen controls key cellular functions of responsive cells including the ability to survive, replicate, communicate and adapt extracellular milieu. Changes in expression 8400 genes were monitored here by cDNA microarray analysis during first 32 h human breast cancer (BC) ZR-75.1 cell stimulation with a mitogenic dose 17beta-estradiol, timing which corresponds completion full mitotic cycle hormone-stimulated cells. Hierarchical clustering 344 whose either increases or decreases...
Immunoglobulins (Igs) are fundamental components of the adaptive immune system vertebrates, with IgT/IgZ isotype specific Teleosts. In this paper we describe identification an IgT heavy chain from European sea bass (Dicentrarchus labrax L.), its molecular characterization and tissue mRNA localization by in situ hybridization. Sea consists 552 aa (Accession Number KM410929) it contains a putative 19 amino acids long signal peptide one potential N-glycosylation site. The C-region four CH...