A5007398514- CRISPR and Genetic Engineering
- Plant tissue culture and regeneration
- Chromosomal and Genetic Variations
- Genetically Modified Organisms Research
- Plant Parasitism and Resistance
- Plant Genetic and Mutation Studies
- Plant Molecular Biology Research
- Plant and animal studies
- Transgenic Plants and Applications
- Plant Virus Research Studies
- Plant Pathogens and Resistance
- Weed Control and Herbicide Applications
- Agricultural pest management studies
- Animal Genetics and Reproduction
- Agronomic Practices and Intercropping Systems
- Photosynthetic Processes and Mechanisms
- Legume Nitrogen Fixing Symbiosis
- Insect Resistance and Genetics
- Plant Disease Resistance and Genetics
- Pesticide and Herbicide Environmental Studies
- Innovation and Socioeconomic Development
- Plant pathogens and resistance mechanisms
- Agriculture, Plant Science, Crop Management
- Advanced biosensing and bioanalysis techniques
- Plant biochemistry and biosynthesis
University of Naples Federico II
2024
Research Centre for Cereal and Industrial Crops
2017-2024
Federico II University Hospital
2024
Institute of Biosciences and Bioresources
2022
University of Perugia
2007-2017
National Agency for New Technologies, Energy and Sustainable Economic Development
2015-2016
Swedish University of Agricultural Sciences
2015
Climate change, associated with global warming, extreme weather events, and increasing incidence of weeds, pests pathogens, is strongly influencing major cropping systems. In this challenging scenario, miscellaneous strategies are needed to expedite the rate genetic gains purpose developing novel varieties. Large plant breeding populations, efficient high-throughput technologies, big data management tools, downstream biotechnology molecular techniques pillars on which next generation based....
We have established a DNA-free genome editing method via ribonucleoprotein-based CRISPR/Cas9 in cultivated tomato and obtained mutant plants regenerated from transfected protoplasts with high mutation rate. The application of as research breeding has provided many possibilities to improve traits crops recent years. In (Solanum lycopersicum), so far only stable Agrobacterium-mediated transformation carrying reagents been established. Shoot regeneration is the major bottleneck tomato. this...
Abstract Parasitic weed infestations pose an increasing threat to agriculture worldwide, especially in the Mediterranean region. Phelipanche ramosa and P. aegyptiaca (broomrapes) cause severe damage field-grown tomato ( Solanum lycopersicum L. ). Strigolactones (SLs), apocarotenoid phytohormones, play a critical role plant physiology development, are also primary signals that trigger germination of parasitic seeds. We generated CRISPR/Cas9 knock-out lines for SlD27 gene, as well three other...
Abstract Background The aim of this study was to evaluate and characterize the mutations induced by two TALE-based approaches, double-strand break (DSB) induction Fok I nuclease (mitoTALEN) targeted base editing DddA cytidine deaminase (mitoTALECD), edit, for first time, mitochondrial genome potato, a vegetatively propagated crop. methods were used knock out same target sequence ( orf125 ). Results Targeted chondriome deletions different sizes (236–1066 bp) mitoTALEN due DSB repair through...
Summary Genic‐cytoplasmic male sterility (CMS) due to interactions between nuclear and cytoplasmic genomes is a well‐known phenomenon in some Solanum spp. hybrids, but genes involved are not known. In this study, the chondriomes of two isonuclear male‐fertile sterile somatic hybrids (SH9A SH9B, respectively) common potato ( S. tuberosum Group Tuberosum , tbr ) wild species commersonii were sequenced compared those parental identify mitochondrial expression sterility. A putative novel gene...
Vegetables include high-value crops with health-promoting effects and reduced environmental impact. The availability of genomic biotechnological tools in certain species, coupled the recent development new breeding techniques based on precise editing DNA, provides unique opportunities to finally take advantage past decades detailed genetic analyses, thus making improvement traits related quality stress tolerance achievable a reasonable time frame. Recent reports such approaches vegetables...
The molecular mechanisms of transferred DNA (T-DNA) integration into the plant genome are still not completely understood. A large number events have been analyzed in different species, shedding light on involved, and frequent transfer vector sequences outside T-DNA borders, so-called backbone (VB) sequences. In this work, we characterized 46 transgenic alfalfa (Medicago sativa L.) plants (events), generated previous works, for presence VB tracts, sequenced several T-DNA/genomic (gDNA)...
Abstract Main conclusion Simultaneous genome editing of the two homeologous LCYe and ZEP genes Nicotiana benthamiana results in plants which all xanthophylls are replaced by zeaxanthin . Plant carotenoids act both as photoreceptors photoprotectants photosynthesis precursors apocarotenoids, include signaling molecules such abscisic acid (ABA). As dietary components, lutein have photoprotective functions human macula. We developed transient stable combinatorial methods, followed direct LC–MS...
ABSTRACT Transferring a transgenic trait from lab genotype into cultivated plant variety requires selection of single‐locus T 0 plants, backcrossing breeding program with progeny at each cycle and, generally, attaining the homozygous state for transgene locus. Polyploidy and outcrossing complicate this process, as in case alfalfa, an autotetraploid, cross‐fertilizing forage legume. Recently, new, efficient selectable marker gene, MsGSAgr , derived alfalfa glutamate 1‐semialdehyde...
Do you know that there are plants steal food from other plants, damaging and even killing them? Are curious about how why they do it? Parasitic unwelcome guests in agricultural fields can be extremely hard to get rid of, costing farmers a lot of money. In this article we will tell parasitic scientists trying fight them.