A5051183451- Celiac Disease Research and Management
- Photosynthetic Processes and Mechanisms
- Microscopic Colitis
- Algal biology and biofuel production
- Wheat and Barley Genetics and Pathology
- Plant Pathogens and Fungal Diseases
- Plant Molecular Biology Research
- Plant Pathogens and Resistance
- Plant Reproductive Biology
- Agricultural Practices and Plant Genetics
- Helicobacter pylori-related gastroenterology studies
- Plant Virus Research Studies
- Genetic Mapping and Diversity in Plants and Animals
- T-cell and B-cell Immunology
- Silkworms and Sericulture Research
- Genetic diversity and population structure
- Phytase and its Applications
- Saffron Plant Research Studies
- Diabetes and associated disorders
- Plant Disease Resistance and Genetics
- Genetics and Plant Breeding
- Photoreceptor and optogenetics research
- Galectins and Cancer Biology
- Digestive system and related health
- Plant nutrient uptake and metabolism
National Agency for New Technologies, Energy and Sustainable Economic Development
2011-2024
University of Bari Aldo Moro
2024
ENEA Casaccia Research Centre
2011-2020
Università degli Studi della Tuscia
2006-2012
Plant Industry
2006
Commonwealth Scientific and Industrial Research Organisation
2006
Telethon Institute Of Genetics And Medicine
2002-2004
Sapienza University of Rome
1991-1998
Istituto Giannina Gaslini
1996
University of Chieti-Pescara
1992
Significance Saffron is a triploid, sterile species whose red stigmas constitute the most expensive spice on Earth. The color, taste, and aroma of are owed to crocus-specific apocarotenoid accumulation crocetin/crocins, picrocrocin, safranal. Through deep transcriptome analysis, we identified novel carotenoid cleavage dioxygenase (CCD) expression profile parallels production crocetin. Using in bacterio , vitro, planta functional assays, demonstrate that CCD2 catalyzing first dedicated step...
Background The E7 protein of the Human Papillomavirus (HPV) type 16, being involved in malignant cellular transformation, represents a key antigen for developing therapeutic vaccines against HPV-related lesions and cancers. Recombinant production this vaccine an active form compliance with good manufacturing practices (GMP) plays crucial role effective vaccines. E7-based produced plants have been shown to be tumor regression protection pre-clinical models. However, some drawbacks whole-plant...
Single Primer Enrichment Technology (SPET) is a new, robust and customizable solution for targeted genotyping. Unlike genotyping by sequencing, like DNA chips, SPET technology, relying on the sequencing of region flanking primer. Its reliance single primers, rather than primer pairs, greatly simplifies panel design allows higher levels multiplexing PCR-based Thanks to regions surrounding target SNP, discovery thousands closely linked, novel SNPs. In order assess potential high-throughput in...
In order to assess the effect of HLA region on familiality coeliac disease (CD), we carried out a study 121 CD index cases and 325 first degree relatives. The transmission disequilibrium test confirmed importance HLA-DR3 haplotype in susceptibility. However, different distortion found affected children inheriting maternal or paternal DR3 alleles suggested that sex parent might influence risk conferred by this haplotype. increase siblings individuals relative general population (lambda s)...
Saffron is the dried stigmas of Crocus sativus and most expensive spice in world. Its red color due to crocins, which are apocarotenoid glycosides that accumulate vacuole a level up 10% stigma dry weight. Previously, we characterized first dedicated enzyme crocin biosynthetic pathway, carotenoid cleavage dioxygenase2 (CsCCD2), cleaves zeaxanthin yield crocetin dialdehyde. In this work, identified six putative aldehyde dehydrogenase (ALDH) genes expressed C. stigmas. Heterologous expression...
Compartmentation is a key strategy enacted by plants for the storage of specialized metabolites. The saffron spice owes its red color to crocins, complex mixture apocarotenoid glycosides that accumulate in intracellular vacuoles and reach up 10% dry weight. We developed general approach, based on coexpression analysis, heterologous expression yeast (Saccharomyces cerevisiae), vitro transportomic assays using microsomes total plant metabolite extracts, identification putative vacuolar...
The use of sequencing and genotyping platforms has undergone dramatic improvements, enabling the generation a wealth genomic information. Despite this progress, availability high-quality DNA (gDNA) in sufficient concentrations is often main limitation, especially for third-generation platforms. A variety extraction methods commercial kits are available. However, many these costly frequently give either low yield or low-quality DNA, inappropriate next (NGS) Here, we describe fast inexpensive...
In eukaryotic autotrophs, photosystems are composed of a core moiety, hosting charge separation and electron transport reactions, an antenna system, enhancing light harvesting photoprotection. Chlamydomonas reinhardtii, the major PSII is heterogeneous trimeric complex made up LHCBM1–LHCBM9 subunits. Despite high similarity, specific functions have been reported for several members including LHCBM1, 2, 7, 9. this work, we analyzed function LHCBM4 LHCBM6 gene products in vitro by synthesizing...
Abstract: Polymorphism in the 5′‐upstream regulatory region of DQA1 gene has been recently described. Using PCR‐SSO method and SSCP analysis we have investigated this polymorphism a group 111 Italian blood donors which had oligotyped for DRB1, DQB1 genes. Eight allelic variants were detected. Looking at relationships among QAP sequences DRB1 genes, three alternative situations found: 1. one‐to‐one relation between alleles, independently other class II genes; 2. same allele association with...
Chlamydomonas reinhardtii is an unicellular green alga used for functional genomics studies and heterologous protein expression. A major hindrance in these the low level instability of expression nuclear transgenes, due to their rearrangement and/or silencing over time.We constructed dedicated vectors Agrobacterium-mediated transformation carrying, within T-DNA borders, Paromomycin (Paro) selectable marker cassette containing Luciferase (Luc) reporter gene. These newly developed...
Crocins are glycosylated apocarotenoids with strong coloring power and anti-oxidant, anticancer, neuro-protective properties. We previously dissected the saffron crocin biosynthesis pathway, demonstrated that CsCCD2 enzyme, catalyzing carotenoid cleavage step, shows a preference for xanthophyll zeaxanthin in vitro bacterio. In order to investigate substrate specificity planta establish plant-based bio-factory system production, we compared wild-type Nicotiana benthamiana plants, accumulating...
SUMMARY Wild species are an invaluable source of new traits for crop improvement. Over the years, tomato community bred cultivated lines that carry introgressions from different tribe to facilitate trait discovery and mapping. The next phase in such projects is find genes drive identified phenotypes. This can be achieved by genotyping a few thousand individuals resulting fine mapping potentially identify causative gene. To couple mapping, we presenting large, recombination‐rich, Backcross...
Background Chlamydomonas reinhardtii is a model system for algal and cell biology used biotechnological applications, such as molecular farming or biological hydrogen production. The metal-responsive CYC6 promoter repressed by copper induced nickel ions. However, induction weak in some strains, poorly reversible chelating agents like EDTA, causes, at high concentrations, toxicity side effects on growth. Removal of these bottlenecks will encourage the wide use this chemically regulated gene...
Abstract Eggplant (Solanum melongena) is a major vegetable crop with great potential for genetic improvement owing to its large and mostly untapped diversity. It closely related over 500 species of Solanum subgenus Leptostemonum that belong primary, secondary, tertiary genepools exhibit wide range characteristics useful eggplant breeding, including traits adaptive climate change. Germplasm banks worldwide hold more than 19 000 accessions species, most which have yet be evaluated....
Abstract We developed a novel eight-way tomato multiparental advanced generation intercross (MAGIC) population to improve the accessibility of relatives genetic resources geneticists and breeders. The interspecific MAGIC (ToMAGIC) was obtained by intercrossing four accessions each Solanum lycopersicum var. cerasiforme pimpinellifolium, which are weedy relative ancestor cultivated tomato, respectively. eight exotic ToMAGIC founders were selected based on representation diversity geographical...
Gliadins and glutenins are the main protein fractions present in wheat gluten. They responsible for technological nutritional quality of based products. In particular, mainly dough visco-elastic properties, whereas gliadins confer extensibility to most important factor triggering celiac disease, major human intolerance monomeric proteins, polymers stabilized by disulfide bonds. Although they have distinctive structural characteristics, it is possible that some become part glutenin fraction...
In the present work, we study genetic control of reproductive traits under different heat stress conditions in two populations inbred lines derived from crosses between S. pimpinellifolium accessions and tomato cultivars (E9×L5 E6203×LA1589). The temperature increase affected traits, especially at extremely high temperatures, where only a few were able to set fruits. Even though relative modest number QTLs was identified, clusters involved responses detected both on chromosomes 1 2....
Abstract Background Chlamydomonas reinhardtii is a model system for the biology of unicellular green algae. Chemically regulated promoters, such as nickel-inducible CYC6 or low CO 2 -inducible CAH1 promoter, may prove useful expressing, at precise times during its cell cycle, proteins with relevant biological functions, complementing mutants in genes encoding proteins. To this date, has not been reported above promoters. Results We fused and promoters to an HA-tagged RSP3 gene, protein...
To investigate the prevalence and clinical genetic patterns of celiac disease (CD) among siblings CD patients, 103 one twin 80 children were evaluated by means their history, physical examination, blood indices nutritional status, antigliadin antibodies (AGA). Antiendomysium antibody (AEA) levels determined in 70 patients 85 subjects human leucocyte antigen (HLA) typed. On basis or laboratory data both, 21 (20.2%) submitted to intestinal biopsy, whereas biopsy six with positive serologic...