- Plant biochemistry and biosynthesis
- Plant Gene Expression Analysis
- Postharvest Quality and Shelf Life Management
- Horticultural and Viticultural Research
- Plant Virus Research Studies
- Plant Molecular Biology Research
- Insect-Plant Interactions and Control
- Antioxidant Activity and Oxidative Stress
- Fermentation and Sensory Analysis
- Plant Physiology and Cultivation Studies
- Plant Parasitism and Resistance
- Saffron Plant Research Studies
- Plant and animal studies
- Plant Stress Responses and Tolerance
- Insect and Pesticide Research
- Photosynthetic Processes and Mechanisms
- Plant tissue culture and regeneration
- Essential Oils and Antimicrobial Activity
- Polyamine Metabolism and Applications
- Berry genetics and cultivation research
- GABA and Rice Research
- Phytochemicals and Antioxidant Activities
- Plant Reproductive Biology
- Insect Pest Control Strategies
- Genetic Mapping and Diversity in Plants and Animals
Universitat Jaume I
2019-2025
Consejo Superior de Investigaciones Científicas
2012-2024
Instituto de Biología Molecular y Celular de Plantas
2015-2024
Universitat Politècnica de València
2014-2024
University of Castilla-La Mancha
2013-2016
John Innes Centre
2009
Modern commercial tomato varieties are substantially less flavorful than heirloom varieties. To understand and ultimately correct this deficiency, we quantified flavor-associated chemicals in 398 modern, heirloom, wild accessions. A subset of these accessions was evaluated consumer panels, identifying the that made most important contributions to flavor liking. We found modern contain significantly lower amounts many older Whole-genome sequencing a genome-wide association study permitted...
Saffron, the processed stigma of <i>Crocus sativus</i>, is characterized by presence several apocarotenoids that contribute to color, flavor, and aroma spice. However, little known about synthesis compounds during development <i>C. sativus</i> stigma. The developing nearly odorless, but before at anthesis, aromatic compound β-ionone becomes principal norisoprenoid volatile in In this study, four carotenoid cleavage dioxygenase (CCD) genes, <i>CsCCD1a</i>, <i>CsCCD1b</i>, <i>CsCCD4a</i>,...
Abstract Improvement of strawberry (Fragaria × ananassa) fruit flavor is an important goal in breeding programs. To investigate genetic factors controlling this complex trait, a mapping population derived from genotype ‘1392’, selected for its superior flavor, and ‘232’ was profiled volatile compounds over 4 years by headspace solid phase microextraction coupled to gas chromatography mass spectrometry. More than 300 were detected, which 87 identified comparison spectrum retention time those...
The volatile composition of fruit from four Citrus varieties (Powell Navel orange, Clemenules mandarine, and Fortune mandarine Chandler pummelo) covering different species has been studied. Over one hundred compounds were profiled after HS-SPME-GC-MS analysis, including 27 esters, 23 aldehydes, 21 alcohols, 13 monoterpene hydrocarbons, 10 ketones, 5 sesquiterpene 4 cyclic ethers, furans, 2 aromatic which all confirmed with standards. differences in the profile among juices these essentially...
Phenylpropanoid volatiles are responsible for the key tomato fruit (Solanum lycopersicum) aroma attribute termed "smoky." Release of these from their glycosylated precursors, rather than biosynthesis, is major determinant smoky in cultivated tomato. using a combinatorial omics approach, we identified non-smoky glycosyltransferase1 (NSGT1) gene. Expression NSGT1 induced during ripening, and encoded enzyme converts cleavable diglycosides smoky-related phenylpropanoid into noncleavable...
Abstract Low temperature is a major environmental stress that seriously compromises plant development, distribution and productivity. Most crops are from tropical origin and, consequently, chilling sensitive. Interestingly, however, some plants, able to augment their tolerance when previously exposed suboptimal growth temperatures. Yet, the molecular physiological mechanisms underlying this adaptive process, termed acclimation, still remain practically unknown. Here, we demonstrate tomato...
Abstract Plants are frequently subjected to different combinations of abiotic stresses, such as high light (HL) intensity, and elevated temperatures. These environmental conditions pose a threat agriculture production, affecting photosynthesis, decreasing yield. Metabolic responses plants, alterations in carbohydrates amino acid fluxes, play key role the successful acclimation plants directing resources toward stress responses, suppressing growth. Here we show that primary metabolic response...
Mediterranean basin and other citrus-growing areas, such as Florida or California, are among the most vulnerable regions to impacts of global warming. Therefore, citrus will be likely subjected increasing periods water scarcity combined with high temperatures that impair plant growth, development yield. In industry, grafting is used improve varietal characteristics, abiotic stress tolerance. Previous research showed under drought heat conditions, Carrizo citrange (Poncirus trifoliata ×...
Numerous studies have revealed the extent of genetic and phenotypic variation between both species cultivars tomato. Using a series tomato lines resulting from crosses cherry three independent large fruit cultivar (Levovil, VilB, VilD), extensive profiling central primary metabolism volatile organic components was performed. In this study, it possible to define number quantitative trait loci (QTLs) which determined levels metabolites and/or evaluate their co-location with previously defined...
Abstract Virus-induced gene silencing (VIGS) is a powerful tool for reverse genetics in tomato (Solanum lycopersicum). However, the irregular distribution of effects VIGS hampers identification and quantification nonvisual phenotypes. To overcome this limitation, visually traceable system was developed fruit, comprising two elements: (1) transgenic line (Del/Ros1) expressing Antirrhinum majus Delila Rosea1 transcription factors under control fruit-specific E8 promoter, showing...
Polyamine oxidases (PAOs) are flavin-dependent enzymes involved in polyamine catabolism. In Arabidopsis five PAO genes (AtPAO1-AtPAO5) have been identified which present some common characteristics, but also important differences primary structure, substrate specificity, subcellular localization, and tissue-specific expression pattern, may suggest distinct physiological roles. the work, AtPAO5, only so far uncharacterized AtPAO is specifically expressed vascular system, was partially...
Volatile organic compounds (VOCs) are major determinants of fruit flavor, a primary objective in tomato breeding. A recombinant inbred line (RIL) population consisting 169 lines derived from cross between Solanum lycopersicum and red-fruited wild species pimpinellifolium accession (SP) was characterized for VOCs three different seasons. Correlation hierarchical cluster analyses were performed on the 52 identified, providing tool putative assignation individual to metabolic pathways....
Soil flooding is a compound abiotic stress that alters soil properties and limits atmospheric gas diffusion (O 2 CO ) to the roots. The involvement of abscisic acid (ABA) in regulation flooding-specific genetic metabolic responses has been scarcely studied despite its key importance as regulator other conditions. To attain this objective, wild type ABA-deficient tomatoes were subjected short-term (24 h) waterlogging. After period, exchange parameters reduced but not plants always had higher...
Crocins and picrocrocin are high-value hydrophilic pigments produced in saffron used commercially the food pharmaceutical industries. These apocarotenoids derived from oxidative cleavage of zeaxanthin by specific carotenoid dioxygenases. The pathway for crocins biosynthesis was introduced into tomato using fruit constitutive promoters resulted 14.48 mg/g 2.92 DW, without compromising plant growth. strategy involved expression CsCCD2L to produce crocetin dialdehyde...
Abstract BACKGROUND In addition to their services as predators, mirid predators are able induce plant defences by phytophagy. However, whether this induction occurs in sweet pepper and it could be an additional benefit role a biological control agent crop remain unknown. Here, these questions were investigated two model insects, the mirids Nesidiocoris tenuis Macrolophus pygmaeus . RESULTS Plant feeding behaviour was observed both N. M. on occupied 33% 14% of total time spent plant,...
Aroma profile and carotenoids content of melon flesh are two important aspects influencing the quality this fruit that have been characterized using only selected genotypes. However, extant variability whole species remains unknown.A complete view volatile/carotenoid profiles was obtained analyzing 71 accessions, representing diversity species. Gas chromatography-mass spectrometry high-performance liquid chromatography were used to analyze 200 volatile compounds five carotenoids. Genotypes...
Eugenol is a volatile that serves as an attractant for pollinators of flowers, acts defense compound in various plant tissues, and contributes to the aroma fruits. Its production cultivated species such strawberry (Fragaria × ananassa), therefore, important viability quality fruit. We have identified functionally characterized three complementary DNAs (cDNAs) encode proteins with high identity eugenol synthases from several species. Based on sequence comparison wild relative Fragaria vesca,...
The volatile composition of wild strawberry (Fragaria vesca) fruit differs from that the cultivated strawberry, having more intense and fruity aromas. Over last few years, diploid F. vesca has been recognized as a model species for genetic studies (F. x ananassa), here previously developed vesca/F. bucharica Near Isogenic Line collection (NIL) was used to explore variability quality traits. Analysis volatiles by GC-MS in our NIL revealed complex highly variable profile. One hundred compounds...
Plants and insects have coexisted for million years evolved a set of interactions which affect both organisms at different levels. developed various morphological biochemical adaptations to cope with herbivores attacks. However, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) has become the major pest threatening tomato crops worldwide without appropriated management it can cause production losses between 80 100%. The aim this study was investigate in vivo effect serine proteinase...
Volatile organic compounds (VOCs) emitted by plants are secondary metabolites that mediate the plant interaction with pathogens and herbivores. These may perform direct defensive functions, i.e. acting as antioxidant, antibacterial or antifungal agents, indirectly signalling activation of plant's responses. Using a non-targeted GC-MS metabolomics approach, we identified profile VOCs associated differential immune response Rio Grande tomato leaves infected either virulent avirulent strains...