A5087327363- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Plant Virus Research Studies
- Plant biochemistry and biosynthesis
- Plant Pathogenic Bacteria Studies
- Plant Parasitism and Resistance
- Essential Oils and Antimicrobial Activity
- Plant and Fungal Interactions Research
- Phytoplasmas and Hemiptera pathogens
- Insect-Plant Interactions and Control
- Toxin Mechanisms and Immunotoxins
- Endoplasmic Reticulum Stress and Disease
- Allelopathy and phytotoxic interactions
- Food composition and properties
- Piperaceae Chemical and Biological Studies
- Photosynthetic Processes and Mechanisms
- Ubiquitin and proteasome pathways
- Plant Reproductive Biology
- Biofuel production and bioconversion
- Plant Genetic and Mutation Studies
- Phytase and its Applications
- Seed Germination and Physiology
- Plant Molecular Biology Research
- Microbial Metabolites in Food Biotechnology
- Protein Degradation and Inhibitors
Instituto de Biología Molecular y Celular de Plantas
2013-2024
Consejo Superior de Investigaciones Científicas
2013-2024
Universitat Politècnica de València
2011-2024
Laboratoire des Interactions Plantes Micro-Organismes
2018
National Council for Scientific and Technological Development
2004
Brazilian Agricultural Research Corporation
2004
Hydroxycinnamic acid amides (HCAA) are secondary metabolites involved in plant development and defense that have been widely reported throughout the kingdom. These phenolics show antioxidant, antiviral, antibacterial, antifungal activities. Hydroxycinnamoyl-CoA:tyramine N-hydroxycinnamoyl transferase (THT) is key enzyme HCAA synthesis induced response to pathogen infection, wounding, or elicitor treatments, preceding accumulation. We engineered transgenic tomato plants overexpressing THT....
Volatile organic compounds (VOCs) emitted by plants are secondary metabolites that mediate the plant interaction with pathogens and herbivores. These may perform direct defensive functions, i.e. acting as antioxidant, antibacterial or antifungal agents, indirectly signalling activation of plant's responses. Using a non-targeted GC-MS metabolomics approach, we identified profile VOCs associated differential immune response Rio Grande tomato leaves infected either virulent avirulent strains...
Secondary metabolites play an important role in the plant defensive response. They are produced as a defence mechanism against biotic stress by providing plants with antimicrobial and antioxidant weapons. In higher plants, majority of secondary accumulate glycoconjugates. Glycosylation is one commonest modifications metabolites, carried out enzymes called glycosyltransferases.Here we provide evidence that previously described tomato wound pathogen-induced glycosyltransferase Twi1 displays...
Viroids are single-stranded, circular, noncoding RNAs that infect plants, causing devastating diseases. In this work, we employed 2D DIGE, followed by MS identification, to analyze the response of tomato plants infected Citrus exocortis viroid (CEVd). Among differentially expressed proteins detected, 45 were successfully identified and classified into different functional categories. Validation results RT-PCR allowed us classify two expression groups. First group included genes with changes...
Cutin and suberin are lipid polyesters deposited in specific apoplastic compartments. Their fundamental roles plant biology include controlling the movement of gases, water solutes, conferring pathogen resistance. Both cutin have been shown to be present Arabidopsis seed coat where they regulate dormancy longevity. In this study, we use accelerated natural ageing assays, glutathione redox potential measures, optical transmission electron microscopy gas chromatography-mass spectrometry...
Abstract Hydroxylated monoterpenes (HMTPs) are differentially emitted by tomato (Solanum lycopersicum) plants resisting bacterial infection. We have studied the defensive role of these volatiles in response to bacteria, whose main entrance is through stomatal apertures. Treatments with some HMTPs resulted closure and pathogenesis-related protein 1 (PR1) induction. Particularly, α-terpineol induced a salicylic acid (SA) abscisic acid-independent manner conferred resistance bacteria....
Abstract Potato spindle tuber viroid (PSTVd) is a plant pathogen naturally infecting economically important crops such as tomato (Solanum lycopersicum). Here, we aimed to engineer plants highly resistant PSTVd and developed several S. lycopersicum lines expressing an artificial microRNA (amiRNA) against (amiR-PSTVd). Infectivity assays revealed that amiR-PSTVd-expressing were not but instead hypersusceptible the viroid. A combination of phenotypic, molecular, metabolic analyses...
Salicylic acid (SA) is a major plant hormone that mediates the defence pathway against pathogens. SA accumulates in highly variable amounts depending on plant-pathogen system, and several enzyme activities participate restoration of its levels. Gentisic (GA) product 5-hydroxylation SA, which catalysed by S5H, an activity regarded as player homeostasis. GA at high levels tomato plants infected Citrus Exocortis Viroid (CEVd), to lesser extend upon Pseudomonas syringae DC3000 pv. (Pst)...
We report that the plant oncoprotein RolA from Agrobacterium rhizogenes acts to stabilize β- glucoronidase (Gus) when two proteins are expressed as a fusion protein in transformed tobacco. The observed 50-fold increase of Gus activity was shown be related accumulation, with no significant changes mRNA abundance, kinetic properties enzyme and thermostability. entire sequence is essential achieve full effect since both N-terminal region, spanning putative reverse signal-anchor nuclear...
ABSTRACT The phytohormone salicylic acid (SA or 2-hydroxybenzoic acid) plays an important role in plant biotic and abiotic responses. Gentisic (GA 2,5-Dihydroxybenzoic acid, 2,5-DHBA) is the product of SA 5-hydroxylation which catalysed by S5H enzyme, also known as DMR6. GA has been described to accumulate at high levels compatible plant-pathogen interactions such tomato plants infected Citrus Exocortis Viroid (CEVd), a much lesser extend upon Pseudomonas syringae DC3000 pv. ( Pst )...
Abstract Background: Secondary metabolites play an important role in the plant defensive response. They are produced as a defence mechanism against biotic stress by providing plants with antimicrobial and antioxidant weapons. In higher plants, majority of secondary accumulate glycoconjugates. Glycosylation is one commonest modifications metabolites, carried out enzymes called glycosyltransferases. Results: Here we provide evidence that previously described tomato wound pathogen-induced...
ABSTRACT Hydroxylated monoterpenes (HMTPs) are differentially emitted by tomato plants efficiently resisting a bacterial infection. We have studied the defensive role of these volatiles in response to bacteria, whose main entrance stomata apertures. Treatments with some HMTPs resulted stomatal closure and PR1 induction. Particularly, α-terpineol induced salicylic (SA) abscisic acid-independent manner, conferred resistance bacteria. Interestingly, transgenic overexpressing or silencing...
Abstract Background: Secondary metabolites play an important role in the plant defensive response. They are produced as a defence mechanism against biotic stress by providing plants with antimicrobial and antioxidant weapons. In higher plants, majority of secondary accumulate glycoconjugates. Glycosylation is one commonest modifications metabolites, carried out enzymes called glycosyltransferases. Results: Here we provide evidence that previously described tomato wound pathogen-induced...
Abstract Background: Secondary metabolites play an important role in the plant defensive response. They are produced as a defence mechanism against biotic stress by providing plants with antimicrobial and antioxidant weapons. In higher plants, majority of secondary accumulate glycoconjugates. Glycosylation is one commonest modifications metabolites, carried out enzymes called glycosyltransferases. Results: Here we provide evidence that previously described tomato wound pathogen-induced...
Abstract Background: Secondary metabolites play an important role in the plant defensive response. They are produced as a defence mechanism against biotic stress by providing plants with antimicrobial and antioxidant weapons. In higher plants, majority of secondary accumulate glycoconjugates. Glycosylation is one commonest modifications metabolites, carried out enzymes called glycosyltransferases. Results: Here we provide evidence that previously described tomato wound pathogen-induced...