Abstract Mutants able to germinate and perform early growth in medium containing a high NaCl concentration were identified during the course of two independent screenings named salt resistant (sre) salobreño (sañ). The sre sañ mutants also high-osmoticum medium, indicating that they are osmotolerant germination assay. Complementation analyses revealed sre1-1, sre1-2, sañ3-1, sañ3-2 alleles abscisic acid (ABA) biosynthesis ABA2 gene. A map-based cloning strategy allowed identification...

Significance N 6 -methyladenosine (m A) modification has been found to constitute an important regulatory mechanism in RNA biology. Unlike mammals and yeast, no component of the m A cellular machinery described plants at present. Although influence m6A suspected occur plant virus cycle, it never proved. Here we have identified a protein with demethylase activity (atALKBH9B) demonstrate that this removes from vitro. Remarkably, abundance on viral genome alfalfa mosaic is influenced by...

Overexpression of a yeast gene, HAL2, allows the cells to tolerate higher than normal extracellular salt concentrations. HAL2 encodes 3′(2′)5′-bisphosphate nucleotidase that serves remove end products sulfate transfer during cellular metabolism. The enzyme is inhibited by lithium and sodium activated potassium. Metabolic systems are sensitive salt, as well those governing osmolyte synthesis ion transport, offer routes which genetic engineering can be used improve tolerance various organisms salt.

We have performed an extensive study on the responses to salt stress in four related Limonium halophytes with different geographic distribution patterns, during seed germination and early vegetative growth. The aims of work were twofold: establish basis for chorology these species, identify relevant mechanisms tolerance dependent control ion transport osmolyte accumulation. Seeds germinated vitro, presence increasing NaCl concentrations, subjected 'recovery germination' tests; percentages...

We have isolated two Arabidopsis thaliana genes, AtHAL3a and AtHAL3b, showing homology with HAL3, a yeast protein which regulates the cell cycle tolerance to salt stress through inhibition of PPZ1 type-1 phosphatase. Expression in hal3 mutants partially complements their LiCl sensitivity, suggesting possible conserved functions between both proteins. AtHAL3b are induced by is most expressed non-stressed plants, particularly seeds. In situ hybridization demonstrates enrichment mRNA seed...

Abstract Introduction – Plants utilise vaious defence mechanisms against their potential biotic stressing agents such as viroids, viruses, bacteria or fungi and abiotic environmental challenges. Among them metabolic alteration is a common response in both compatible incompatible plant–pathogen interactions. However, the identification of changes associated with not an easy task due to complexity metabolome plant response. To address problem complexity, metabolomics approach was employed this...

Eggplant (Solanum melongena) varieties with increased levels of phenolics in the fruit present enhanced functional quality, but may display greater flesh browning. We evaluated 18 eggplant accessions for total content, chlorogenic acid DPPH scavenging activity, polyphenol oxidase (PPO) liquid extract browning, and For all traits we found a high diversity, differences among up to 3.36-fold Variation content accounted only 18.9% 6.0% variation PPO activity was not significantly correlated...

Hydroxycinnamic acid amides (HCAA) are secondary metabolites involved in plant development and defense that have been widely reported throughout the kingdom. These phenolics show antioxidant, antiviral, antibacterial, antifungal activities. Hydroxycinnamoyl-CoA:tyramine N-hydroxycinnamoyl transferase (THT) is key enzyme HCAA synthesis induced response to pathogen infection, wounding, or elicitor treatments, preceding accumulation. We engineered transgenic tomato plants overexpressing THT....

Tomato plants expressing the NahG transgene, which prevents accumulation of endogenous salicylic acid (SA), were used to study importance SA signalling pathway in basal defence against Citrus Exocortis Viroid (CEVd) or Spotted Wilt Virus (TSWV). The lack CEVd- TSWV-infected tomato led an early and dramatic disease phenotype, as compared that observed corresponding parental Money Maker. Addition acibenzolar-S-methyl, a benzothiadiazole (BTH), activates systemic acquired resistance downstream...

Forest species ranges are confined by environmental limitations such as cold stress. The natural range shifts of pine forests due to climate change and proactive-assisted population migration may each be constrained the ability tolerate low temperatures, especially in northern latitudes or high altitudes. aim this study is characterize response cold-tolerant versus cold-sensitive Pinus halepensis (P. halepensis) seedlings at physiological molecular level under controlled conditions identify...

Citrus exocortis viroid (CEVd) and tomato mosaic virus (ToMV), which produce a systemic non-necrotizing infection in (Lycopersicon esculentum cv. Rutgers), strongly induced the accumulation of phenolic compound that we have characterized as 2,5-dihydroxybenzoic acid (gentisic acid, GA) by nuclear magnetic resonance, following purification high-performance liquid chromatography. Levels free total GA increased more than 150-fold response to CEVd ToMV infections. Unlike these infections,...

Summary In order to investigate the effects of a permanent increase in cellular H 2 O on cation homeostasis we have studied T‐DNA insertion mutant Arabidopsis CATALASE gene. This ( cat2‐1 ) exhibits 20% wild‐type leaf catalase activity and accumulates more than wild type under normal growth conditions. addition reduced size, pale green color great reduction secondary roots, exhibited increased sensitivity , NaCl, norspermidine, high light cold stress. On other hand, germination is tolerant...

Inoculation of tomato plants (Solanum lycopersicum cv. Rutgers) with Pseudomonas syringae pv. led to the production a hypersensitive-like response in this pathovar tomato. Accumulation hydroxycinnamic acid amides (HCAA) tyramine (p-coumaroyltyramine and feruloyltyramine) dopamine (p-coumaroyldopamine feruloyldopamine) was detected after bacterial infection. Two them, p-coumaroyldopamine feruloyldopamine, are described for first time. The accumulation HCAA preceded by an increment...

Aroma profile and carotenoids content of melon flesh are two important aspects influencing the quality this fruit that have been characterized using only selected genotypes. However, extant variability whole species remains unknown.A complete view volatile/carotenoid profiles was obtained analyzing 71 accessions, representing diversity species. Gas chromatography-mass spectrometry high-performance liquid chromatography were used to analyze 200 volatile compounds five carotenoids. Genotypes...

The volatile esters of (Z)-3-hexenol with acetic, propionic, isobutyric, or butyric acids are synthesized by alcohol acyltransferases (AAT) in plants. These compounds differentially emitted when tomato plants efficiently resisting an infection Pseudomonas syringae pv. tomato. We have studied the defensive role these green leaf (GLV) response to bacterial infection, analyzing induction resistance mediated GLVs and phenotype upon impaired their biosynthesis. observed that treatments...

Volatile organic compounds (VOCs) emitted by plants are secondary metabolites that mediate the plant interaction with pathogens and herbivores. These may perform direct defensive functions, i.e. acting as antioxidant, antibacterial or antifungal agents, indirectly signalling activation of plant's responses. Using a non-targeted GC-MS metabolomics approach, we identified profile VOCs associated differential immune response Rio Grande tomato leaves infected either virulent avirulent strains...

Background:Citrus Exocortis Viroid (CEVd) is a non-coding RNA pathogen capable of infecting wide range plant species, despite its lack protein-coding ability. infections induce significant alterations in various physiological and biochemical processes, particularly impacting metabolism. This study shows the metabolic changes upon viroid infection tomato plants (Solanum lycopersicum var. ‘MoneyMaker’) exhibiting altered levels salicylic acid (SA), key signal molecule involved defence against...

The salt tolerance of the respiratory yeast Candida tropicalis and fermentative Saccharomyces cerevisiae have been compared in glucose media. C. showed a better adaptation to Na + Li maintained higher intracellular K :Na :Li ratios than S. However, poorer osmotic stress (produced by KCI sorbitol) exhibited reduced glycerol production as In media with non-repressing sugar galactose carbon source, increased sensitivity stress. Under these conditions, , but not utilized trehalose more important...