A5005891238- Plant-Microbe Interactions and Immunity
- Plant Virus Research Studies
- Plant tissue culture and regeneration
- Plant Parasitism and Resistance
- Plant Pathogenic Bacteria Studies
- Plant Reproductive Biology
- Plant biochemistry and biosynthesis
- Essential Oils and Antimicrobial Activity
- Plant Pathogens and Fungal Diseases
- Chromosomal and Genetic Variations
- Toxin Mechanisms and Immunotoxins
- Plant and Fungal Interactions Research
- Insect Resistance and Genetics
- Plant pathogens and resistance mechanisms
- Plant Stress Responses and Tolerance
- Insect-Plant Interactions and Control
- Plant Molecular Biology Research
- Insect and Pesticide Research
- Botanical Research and Applications
- Entomopathogenic Microorganisms in Pest Control
- Coagulation and Flocculation Studies
- Phytoplasmas and Hemiptera pathogens
- Biochemical and biochemical processes
- CRISPR and Genetic Engineering
- Fermentation and Sensory Analysis
Instituto de Biología Molecular y Celular de Plantas
2016-2025
Consejo Superior de Investigaciones Científicas
2016-2025
Universitat Politècnica de València
2016-2025
University of Rochester
2009
Universitat de València
1988
Hydroxycinnamic acid amides (HCAA) are secondary metabolites involved in plant development and defense that have been widely reported throughout the kingdom. These phenolics show antioxidant, antiviral, antibacterial, antifungal activities. Hydroxycinnamoyl-CoA:tyramine N-hydroxycinnamoyl transferase (THT) is key enzyme HCAA synthesis induced response to pathogen infection, wounding, or elicitor treatments, preceding accumulation. We engineered transgenic tomato plants overexpressing THT....
Tomato plants expressing the NahG transgene, which prevents accumulation of endogenous salicylic acid (SA), were used to study importance SA signalling pathway in basal defence against Citrus Exocortis Viroid (CEVd) or Spotted Wilt Virus (TSWV). The lack CEVd- TSWV-infected tomato led an early and dramatic disease phenotype, as compared that observed corresponding parental Money Maker. Addition acibenzolar-S-methyl, a benzothiadiazole (BTH), activates systemic acquired resistance downstream...
The volatile esters of (Z)-3-hexenol with acetic, propionic, isobutyric, or butyric acids are synthesized by alcohol acyltransferases (AAT) in plants. These compounds differentially emitted when tomato plants efficiently resisting an infection Pseudomonas syringae pv. tomato. We have studied the defensive role these green leaf (GLV) response to bacterial infection, analyzing induction resistance mediated GLVs and phenotype upon impaired their biosynthesis. observed that treatments...
Volatile organic compounds (VOCs) emitted by plants are secondary metabolites that mediate the plant interaction with pathogens and herbivores. These may perform direct defensive functions, i.e. acting as antioxidant, antibacterial or antifungal agents, indirectly signalling activation of plant's responses. Using a non-targeted GC-MS metabolomics approach, we identified profile VOCs associated differential immune response Rio Grande tomato leaves infected either virulent avirulent strains...
Background:Citrus Exocortis Viroid (CEVd) is a non-coding RNA pathogen capable of infecting wide range plant species, despite its lack protein-coding ability. infections induce significant alterations in various physiological and biochemical processes, particularly impacting metabolism. This study shows the metabolic changes upon viroid infection tomato plants (Solanum lycopersicum var. ‘MoneyMaker’) exhibiting altered levels salicylic acid (SA), key signal molecule involved defence against...
As a response to the stress induced by different afflicting agents, tomato plants ( Lycopersicon esculentum ) produce so‐called ‘pathogenesis‐related’ proteins. Here we report degradation of some these proteins constitutive endoproteolytic activity that co‐distributes with pathogenesis‐related in intercellular spaces leaves infected citrus exocortis viroid. This endoproteinase was purified, showing pH optimum 2.5–3.5, M r 37000 and selective inhibition pepstatin. In crude homogenates, enzyme...
Tobacco (Nicotiana tabacum L.) leaves were found to contain an extracellular proteinase that endoproteolytically cleaves tobacco pathogenesis-related (PR) proteins. This was partially purified from and characterized as aspartyl with a pH optimum around 3 molecular mass of 36,000 40,000 daltons. In vitro, the enzyme cleaved tomato PR proteins into discrete fragments. The characteristics this similar pepsin identical those displayed by previously described 37-kilodalton active against (I...
Abstract Viroids are naked RNAs that do not code for any known protein and yet able to infect plants causing severe diseases. Because of their RNA nature, many studies have focused on the involvement viroids in RNA-mediated gene silencing as being pathogenesis mechanism. Here, alterations caused by Citrus exocortis viroid (CEVd) tomato translation machinery were studied a new aspect pathogenesis. The presence ribosomal fractions infected was detected. More precisely, CEVd its derived small...
New strategies of control need to be developed with the aim economic and environmental sustainability in plant crop protection. Metabolomics is an excellent platform for both understanding complex plant-pathogen interactions unraveling new chemical strategies. GC-MS-based metabolomics, along a phytohormone analysis compatible incompatible interaction between tomato plants
Abstract Proteinaceous aspartic proteinase inhibitors are rare in nature and described only a few plant species. One of them corresponds to family cathepsin D (CDIs) potato (Solanum tuberosum), involving up 15 isoforms with high sequence similarity. In this work, we describe tomato lycopersicum) wound-inducible protein called jasmonic-induced 21 (JIP21). Sequence analysis its cDNA predicted putative function as CDI. The JIP21 gene, whose has been demonstrated be glycosylated, is...
Secondary metabolites play an important role in the plant defensive response. They are produced as a defence mechanism against biotic stress by providing plants with antimicrobial and antioxidant weapons. In higher plants, majority of secondary accumulate glycoconjugates. Glycosylation is one commonest modifications metabolites, carried out enzymes called glycosyltransferases.Here we provide evidence that previously described tomato wound pathogen-induced glycosyltransferase Twi1 displays...
A 23-kD pathogenesis-related protein (P23) is induced in tomato (Lycopersicon esculentum Mill, cv Rutgers) plants when infected with citrus exocortis viroid. This homologous to the salt-induced NP24 (I. Rodrigo, P. Vera, R. Frank, V. Conejero [1991] Plant Mol Biol 16: 931-934). Further characterization of P23 has shown that this accumulates vacuoles association dense inclusion bodies. In vitro assays indicated purified inhibits growth several phytopathogenic fungi. P23-coding cDNA clones...
We describe microneutralization assays that used automated 96-well enzyme-linked immunospot (ELI-SPOT) readout instrumentation to measure human anti-dengue virus (DENV) antibodies in CV-1 cells were stably transfected express FcγRIIA (CD32) using conventional Vero as a comparator. Classic plaque reduction neutralization test (PRNT) end-point titers determined by probit analysis. Neutralization against DENV measured transfectants expressed terms of both 50% 90% PRNT and differential...
Viroids are single-stranded, circular, noncoding RNAs that infect plants, causing devastating diseases. In this work, we employed 2D DIGE, followed by MS identification, to analyze the response of tomato plants infected Citrus exocortis viroid (CEVd). Among differentially expressed proteins detected, 45 were successfully identified and classified into different functional categories. Validation results RT-PCR allowed us classify two expression groups. First group included genes with changes...
Moringa oleifera seeds contain a cationic coagulant protein that can be used either for drinking water clarification or wastewater treatment. The 30–40% (w/w) oil, which represents valuable resource with industrial, food and renewable energy applications. However, the oil also increases organic matter added to avoids storage of treated its consumption within 24 h. So extraction is recommended as way purify extract. This work proposes solvent using three solvents (hexane, acetone, ethanol)...