A5018975279- Plant Physiology and Cultivation Studies
- Phytoplasmas and Hemiptera pathogens
- Genomics and Phylogenetic Studies
- Plant Reproductive Biology
- Plant Pathogens and Fungal Diseases
- Cocoa and Sweet Potato Agronomy
- Plant Molecular Biology Research
- Banana Cultivation and Research
- Plant Disease Resistance and Genetics
- Plant Gene Expression Analysis
- Chromosomal and Genetic Variations
- Plant Virus Research Studies
- Developmental Biology and Gene Regulation
- Photosynthetic Processes and Mechanisms
- Postharvest Quality and Shelf Life Management
- Genetic Mapping and Diversity in Plants and Animals
- Plant Stress Responses and Tolerance
- Plant and soil sciences
- Plant biochemistry and biosynthesis
- Rice Cultivation and Yield Improvement
- Plant Pathogenic Bacteria Studies
- CRISPR and Genetic Engineering
- Pineapple and bromelain studies
- Neurobiology and Insect Physiology Research
- Plant nutrient uptake and metabolism
Universitat Politècnica de València
2008-2023
Instituto Valenciano de Investigaciones Agrarias
2010-2022
Valencian Infertility Institute
2015
Sistemas Genómicos
2002-2012
Universitat de València
1998-2008
Imperial College London
1995
Functional genomics technologies have been widely adopted in the biological research of both model and non-model species. An efficient functional annotation DNA or protein sequences is a major requirement for successful application these approaches as information on gene products often key to interpretation experimental results. Therefore, there an increasing need bioinformatics resources which are able cope with large amount sequence data, produce valuable results easily accessible...
The genus Citrus, comprising some of the most widely cultivated fruit crops worldwide, includes an uncertain number species. Here we describe ten natural citrus species, using genomic, phylogenetic and biogeographic analyses 60 accessions representing diverse germ plasms, propose that diversified during late Miocene epoch through a rapid southeast Asian radiation correlates with marked weakening monsoons. A second enabled by migration across Wallace line gave rise to Australian limes in...
Genome sequences of nine species citrus, including oranges, pummelos and mandarins, reveal pathways domestication provide resources for breeding. Cultivated citrus are selections from, or hybrids of, wild progenitor whose identities contributions to remain controversial. Here we sequence compare genomes—a high-quality reference haploid clementine genome mandarin, pummelo, sweet-orange sour-orange genomes—and show that cultivated types derive from two species. Although represent one species,...
Abstract We have identified two genes from Arabidopsis that show high similarity withCBF1, a gene encoding an AP2 domain-containing transcriptional activator binds to the low-temperature-responsive element CCGAC and induces expression of some cold-regulated genes, increasing plant freezing tolerance. These which we named CBF2 CBF3, also encode proteins containing DNA-binding motifs. Furthermore, like CBF1, CBF3 include putative nuclear-localization signals potential acidic activation...
Citrus genus includes some of the most important cultivated fruit trees worldwide. Despite being extensively studied because its commercial relevance, origin citrus species and history domestication still remain an open question. Here, we present a phylogenetic analysis chloroplast genomes 34 genotypes which constitutes comprehensive detailed study to date on evolution variability Citrus. A statistical model was used estimate divergence times between major groups. Additionally, complete map...
During the last decade, numerous microsatellite markers were developed for genotyping and to identify closely related plant genotypes. In citrus, previously arisen from genomic libraries more often located in non coding DNA sequences. To optimize use of these EST-SSRs as genetic genome mapping programs citrus systematic analysis, we have investigated their polymorphism type (di or trinucleotide) position Among 11000 unigenes a Clementine EST library, found at least one sequence (repeated...
Most modern citrus cultivars have an interspecific origin. As a foundational step towards deciphering the genome structures, reference whole sequence was produced by International Citrus Genome Consortium from haploid derived Clementine mandarin. The availability of saturated genetic map identified as essential prerequisite to assist assembly. is believed be 'Mediterranean' mandarin × sweet orange hybrid, and likely arose hybridizations between pummelo gene pools. primary goals present study...
With the increasing availability of EST databases and whole genome sequences, SNPs have become most abundant powerful polymorphic markers. However, SNP chip data generally suffers from ascertainment biases caused by discovery selection process in which a small number individuals are used as panels. The ongoing International Citrus Genome Consortium sequencing project highly heterozygous Clementine sweet orange genomes will soon result release several hundred thousand SNPs. primary goals this...
After its domestication, rice cultivation expanded from tropical regions towards northern latitudes with temperate climate in a progressive process to overcome limiting photoperiod and temperature conditions. This has originated wide range of diversity that can be regarded as valuable resource for crop improvement. In general, current breeding programs have deal lack both germplasm accessions specifically adapted local agro-environmental conditions donors carrying desired agronomical traits....
To better understand the molecular and physiological mechanisms underlying maintenance release of seasonal bud dormancy in perennial trees, we identified differentially expressed genes during progression reproductive buds from peach (Prunus persica [L.] Batsch) by suppression subtractive hybridization (SSH) microarray hybridization. Four SSH libraries were constructed, which respectively enriched cDNA highly dormant (named DR), dormancy-released (RD) cultivars with different chilling...
Abstract Background Citrus species constitute one of the major tree fruit crops subtropical regions with great economic importance. However, their peculiar reproductive characteristics, low genetic diversity and long-term nature breeding mostly impair citrus variety improvement. In woody plants, genomic science holds promise improvements in genera development tools may be crucial for further crop improvements. this work we report characterization three BAC libraries from Clementine (...
• Premise of the study: Microsatellite primers were developed from bacterial artificial chromosome (BAC) end sequences Citrus clementina and their transferability polymorphism tested in genus for future anchorage physical genetic maps comparative interspecific mapping. Methods Results: Using PAGE DNA silver staining, 79 primer pairs selected among 526 microsatellites mined BES. A preliminary diversity study was conducted with 18 them, C. reticulata , maxima medica sinensis aurantium paradisi...
Abstract Background Improvement of Citrus , the most economically important fruit crop in world, is extremely slow and inherently costly because long-term nature tree breeding an unusual combination reproductive characteristics. Aside from disease resistance, major commercial traits are improved quality, higher yield tolerance to environmental stresses, especially salinity. Results A normalized full length 9 standard cDNA libraries were generated, representing particular treatments tissues...
Abstract Background Research on citrus fruit ripening has received considerable attention because of the importance fruits for human diet. Organic acids are among main determinants taste and organoleptic quality hence control acidity loss a strong economical relevance. In citrus, organic accumulate in juice sac cells developing catabolized thereafter during ripening. Aconitase, that transforms citrate to isocitrate, is first step citric acid catabolism major component utilization machinery....
The citrus genus comprises a number of sensitive tropical and subtropical species to cold stress, which limits global distribution certain latitudes causes major economic loss. We used RNA-Seq technology analyze changes in the transcriptome Valencia delta seedless orange response long-term stress grafted on two frequently rootstocks: Carrizo citrange (CAR), considered one most cold-tolerant accessions; C. macrophylla (MAC), very one. Our objectives were identify genetic mechanism that...
Abstract Background Abscission is an active, organized, and highly coordinated cell separation process enabling the detachment of aerial organs through modification cell-to-cell adhesion breakdown walls at specific sites on plant body known as abscission zones. In Arabidopsis thaliana , floral cauline leaves regulated by interaction hormonal peptide INFLORESCENCE DEFICIENT IN ABSCISSION (IDA), a pair redundant receptor-like protein kinases, HAESA (HAE) HAESA-LIKE2 (HSL2), SOMATIC...
Sequencing of the mitochondrial cytochrome b gene has been used to differentiate three tuna species: Thunnus albacares (yellowfin tuna), obesus (bigeye and Katsuwonus pelamis (skipjack). A PCR amplified 528 bp fragment from 30 frozen samples a 171 26 canned species were analyzed determine intraspecific variation positions with diagnostic value. Polymorphic sites between that did not present given The genetic distance sequences was calculated, phylogenetic tree constructed, showing belonging...
SUMMARY Sharka disease, caused by the Plum pox virus (PPV), is one of main limiting factors for stone fruit crops worldwide. Only a few resistance sources have been found in apricot ( Prunus armeniaca L.), and most studies located major PPV locus PPVres ) on linkage group 1 (LG1). However, mapping accuracy was not sufficiently reliable predicted within low confidence interval. In this study, we constructed two high‐density simple sequence repeat (SSR) improved maps with 0.70 0.68 markers/cm,...
Harvest time is a relevant economic trait in citrus, and selection of cultivars with different fruit maturity periods has remarkable impact the market share. Generation early- late-maturing an important target for citrus breeders, therefore, generation knowledge regarding genetic mechanisms controlling ripening process causing early late phenotypes crucial. In this work we analyze evolution transcriptome during 3 sport mutations derived from Fina clementine (Citrus clementina) mandarin:...