A5069920187- Plant-Microbe Interactions and Immunity
- Plant Virus Research Studies
- Plant Parasitism and Resistance
- Plant Pathogenic Bacteria Studies
- Plant tissue culture and regeneration
- Plant biochemistry and biosynthesis
- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Essential Oils and Antimicrobial Activity
- Plant and Fungal Interactions Research
- Chromosomal and Genetic Variations
- RNA and protein synthesis mechanisms
- Plant Reproductive Biology
- Horticultural and Viticultural Research
- Plant and animal studies
- Plant responses to elevated CO2
- Genomics and Phylogenetic Studies
- Phytoplasmas and Hemiptera pathogens
- E-Learning and Knowledge Management
- Insect-Plant Interactions and Control
- Plant Pathogens and Fungal Diseases
- Plant Physiology and Cultivation Studies
- Entomopathogenic Microorganisms in Pest Control
- Health Education and Validation
- Engineering Education and Technology
Instituto de Biología Molecular y Celular de Plantas
2016-2025
Consejo Superior de Investigaciones Científicas
2016-2025
Universitat Politècnica de València
2016-2025
UCLouvain
2011
John Innes Centre
2008
Sainsbury Laboratory
2008
Hydroxycinnamic acid amides (HCAA) are secondary metabolites involved in plant development and defense that have been widely reported throughout the kingdom. These phenolics show antioxidant, antiviral, antibacterial, antifungal activities. Hydroxycinnamoyl-CoA:tyramine N-hydroxycinnamoyl transferase (THT) is key enzyme HCAA synthesis induced response to pathogen infection, wounding, or elicitor treatments, preceding accumulation. We engineered transgenic tomato plants overexpressing THT....
Tomato plants expressing the NahG transgene, which prevents accumulation of endogenous salicylic acid (SA), were used to study importance SA signalling pathway in basal defence against Citrus Exocortis Viroid (CEVd) or Spotted Wilt Virus (TSWV). The lack CEVd- TSWV-infected tomato led an early and dramatic disease phenotype, as compared that observed corresponding parental Money Maker. Addition acibenzolar-S-methyl, a benzothiadiazole (BTH), activates systemic acquired resistance downstream...
The volatile esters of (Z)-3-hexenol with acetic, propionic, isobutyric, or butyric acids are synthesized by alcohol acyltransferases (AAT) in plants. These compounds differentially emitted when tomato plants efficiently resisting an infection Pseudomonas syringae pv. tomato. We have studied the defensive role these green leaf (GLV) response to bacterial infection, analyzing induction resistance mediated GLVs and phenotype upon impaired their biosynthesis. observed that treatments...
Volatile organic compounds (VOCs) emitted by plants are secondary metabolites that mediate the plant interaction with pathogens and herbivores. These may perform direct defensive functions, i.e. acting as antioxidant, antibacterial or antifungal agents, indirectly signalling activation of plant's responses. Using a non-targeted GC-MS metabolomics approach, we identified profile VOCs associated differential immune response Rio Grande tomato leaves infected either virulent avirulent strains...
RNA interference (RNAi)-based tools are used in multiple organisms to induce antiviral resistance through the sequence-specific degradation of target RNAs by complementary small RNAs. In plants, highly specific RNAi-based include artificial microRNAs (amiRNAs) and synthetic trans-acting interfering (syn-tasiRNAs). syn-tasiRNAs have emerged as a promising tool allowing for multi-targeting viral simultaneous expression several from single precursor. Here, we compared tomato plants effects an...
Background:Citrus Exocortis Viroid (CEVd) is a non-coding RNA pathogen capable of infecting wide range plant species, despite its lack protein-coding ability. infections induce significant alterations in various physiological and biochemical processes, particularly impacting metabolism. This study shows the metabolic changes upon viroid infection tomato plants (Solanum lycopersicum var. ‘MoneyMaker’) exhibiting altered levels salicylic acid (SA), key signal molecule involved defence against...
Many plastidial isoprenoids, including diterpenes and photosynthesis-related isoprenoids such as carotenoids chlorophylls, derive from C20 geranylgeranyl diphosphate (GGPP), produced by GGPP synthase (GGPPS) enzymes. Heterodimers of GGPPS non-catalytic type I small subunit (SSU-I) proteins produce C10 geranyl (GPP), the precursor monoterpenes. Three isoforms, referred to SlG1-3, are present in tomato (Solanum lycopersicum). Here we explored their contribution production volatile organic...
Abstract Viroids are naked RNAs that do not code for any known protein and yet able to infect plants causing severe diseases. Because of their RNA nature, many studies have focused on the involvement viroids in RNA-mediated gene silencing as being pathogenesis mechanism. Here, alterations caused by Citrus exocortis viroid (CEVd) tomato translation machinery were studied a new aspect pathogenesis. The presence ribosomal fractions infected was detected. More precisely, CEVd its derived small...
New strategies of control need to be developed with the aim economic and environmental sustainability in plant crop protection. Metabolomics is an excellent platform for both understanding complex plant-pathogen interactions unraveling new chemical strategies. GC-MS-based metabolomics, along a phytohormone analysis compatible incompatible interaction between tomato plants
Protein synthesis in crop plants contributes to the balance of food and fuel on our planet, which influences human metabolic activity lifespan. can be regulated with respect changing environmental cues via deposition chemical modifications into rRNA. Here, we present structure a plant ribosome from tomato quantitative mass spectrometry analysis its rRNAs. The study reveals fine features ribosomal proteins 71 plant-specific rRNA modifications, it re-annotates 30 residues available sequence....
Abstract Proteinaceous aspartic proteinase inhibitors are rare in nature and described only a few plant species. One of them corresponds to family cathepsin D (CDIs) potato (Solanum tuberosum), involving up 15 isoforms with high sequence similarity. In this work, we describe tomato lycopersicum) wound-inducible protein called jasmonic-induced 21 (JIP21). Sequence analysis its cDNA predicted putative function as CDI. The JIP21 gene, whose has been demonstrated be glycosylated, is...
Secondary metabolites play an important role in the plant defensive response. They are produced as a defence mechanism against biotic stress by providing plants with antimicrobial and antioxidant weapons. In higher plants, majority of secondary accumulate glycoconjugates. Glycosylation is one commonest modifications metabolites, carried out enzymes called glycosyltransferases.Here we provide evidence that previously described tomato wound pathogen-induced glycosyltransferase Twi1 displays...
Viroids are small, circular, highly structured pathogens that infect a broad range of plants, causing economic losses. Since their discovery in the 1970s, they have been considered as non-coding pathogens. In last few years, other RNA entities, similar terms size and structure, were shown to be translated (e.g., cirRNAs, precursors miRNA, satellites) well studies showing some viroids located ribosomes, reignited idea may translated. this study, we used advanced bioinformatic analysis, vitro...
Viroids are single-stranded, circular, noncoding RNAs that infect plants, causing devastating diseases. In this work, we employed 2D DIGE, followed by MS identification, to analyze the response of tomato plants infected Citrus exocortis viroid (CEVd). Among differentially expressed proteins detected, 45 were successfully identified and classified into different functional categories. Validation results RT-PCR allowed us classify two expression groups. First group included genes with changes...
Cutin and suberin are lipid polyesters deposited in specific apoplastic compartments. Their fundamental roles plant biology include controlling the movement of gases, water solutes, conferring pathogen resistance. Both cutin have been shown to be present Arabidopsis seed coat where they regulate dormancy longevity. In this study, we use accelerated natural ageing assays, glutathione redox potential measures, optical transmission electron microscopy gas chromatography-mass spectrometry...
Carotenoids are photoprotectant pigments and precursors of hormones such as strigolactones (SL). produced in plastids from geranylgeranyl diphosphate (GGPP), which is diverted to the carotenoid pathway by phytoene synthase (PSY). In tomato (Solanum lycopersicum), three genes encode plastid-targeted GGPP synthases (SlG1 SlG3) PSY isoforms (PSY1 PSY3). Here, we investigated function SlG1 generating loss-of-function lines combining their metabolic physiological phenotyping with gene...
Abstract Hydroxylated monoterpenes (HMTPs) are differentially emitted by tomato (Solanum lycopersicum) plants resisting bacterial infection. We have studied the defensive role of these volatiles in response to bacteria, whose main entrance is through stomatal apertures. Treatments with some HMTPs resulted closure and pathogenesis-related protein 1 (PR1) induction. Particularly, α-terpineol induced a salicylic acid (SA) abscisic acid-independent manner conferred resistance bacteria....
Limonium is a genus represented in the Iberian Peninsula by numerous halophytic species that are affected nature salinity, and often prolonged drought episodes. Responses to water deficit have been studied four Mediterranean species, previously investigated regarding salt tolerance mechanisms. The levels of biochemical markers, associated with specific responses—photosynthetic pigments, mono- divalent ions, osmolytes, antioxidant compounds enzymes—were determined control water-stressed...
Agronomy solutions for modifying pre-harvest grape ripening are needed a more sustainable viticulture. Field experiments were performed in Vitis vinifera L. vines to study the effect of previously described stomata-closing compound (Z)-3-hexenyl butyrate (HB). Exogenous treatments at different doses periodically carried out using randomized block design. Firstly, we observed that HB was able induce stomatal closure grapevine plants. Under field conditions, application around veraison induced...