A5055670383- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- DNA and Nucleic Acid Chemistry
- Plant and Fungal Interactions Research
- RNA Research and Splicing
- RNA modifications and cancer
- Advanced biosensing and bioanalysis techniques
- Bacteriophages and microbial interactions
- Viral Infections and Immunology Research
- Hepatitis C virus research
- RNA Interference and Gene Delivery
- Animal Virus Infections Studies
- Chromosomal and Genetic Variations
- Plant Disease Resistance and Genetics
- HIV Research and Treatment
- CRISPR and Genetic Engineering
- Insect-Plant Interactions and Control
- HIV/AIDS drug development and treatment
- Plant Reproductive Biology
- Cancer-related molecular mechanisms research
- Hepatitis B Virus Studies
- Phytoplasmas and Hemiptera pathogens
- Virus-based gene therapy research
- Bacterial Genetics and Biotechnology
- Chemical Synthesis and Analysis
Université de Sherbrooke
2016-2025
Université des Sciences de la Santé
2012
Département de Chimie Moléculaire
2012
University of Dundee
2006
Cancer Research UK
2006
Canadian Food Inspection Agency
2004
Agriculture and Agri-Food Canada
1995
Yale University
1993
Université de Montréal
1989-1990
Guanine-rich RNA sequences can fold into non-canonical, four stranded helical structures called G-quadruplexes that have been shown to be widely distributed within the mammalian transcriptome, as well being key regulatory elements in various biological mechanisms. That said, their role 3′-untranslated region (UTR) of mRNA remains elucidated and appreciated. A bioinformatic analysis 3′-UTRs mRNAs revealed enrichment G-quadruplexes. To shed light on role(s) these structures, those found LRP5...
Abstract The tomato (Solanum lycopersicum) callose synthase genes CalS11-like and CalS12-like encode proteins that are essential for the formation of callose, a major component pollen mother cell walls; these enzymes also function in during pathogen infection. This article describes targeting mRNAs by small RNA derived from virulence modulating region two Potato spindle tuber viroid variants. More specifically, infection plants resulted suppression target up to 1.5-fold, depending on variant...
MicroRNAs (miRNAs) are small noncoding RNAs that repress the translation of their target genes. It has previously been shown a target's availability to miRNA can be affected by its structure. G-quadruplexes (G4) noncanonical structures adopted G-rich nucleic acids have multiple biological functions. In this study, whether or not G4 structures’ presence in 3′ UTRs mRNAs hinder binding was investigated. Putative overlapping with predicted miRNAs’ sites searched for, and 44,294 hits were found...
G-quadruplex structures in RNA molecules are known to have regulatory impacts cells but difficult locate the genome. The minimal requirements for folding (G≥3N1-7 G≥3N1-7 G≥3) is being challenged by observations made on specific examples recent years. definition of potential sequences has major repercussions observation structure since it introduces a bias. canonical motif only describes sub-population reported G-quadruplexes. To address these issues, we propose an prediction strategy that...
The Y genes encode small noncoding RNAs whose functions remain elusive, numbers vary between species, and major property is to be bound by the Ro60 protein (or its ortholog in other species). To better understand evolution of gene family, we performed a homology search 27 different genomes along with structural using RNA specific motifs. These searches confirmed that are well conserved animal kingdom resulted detection several new genes, including first insects second detected Caenorhabditis...
Viroids are small, circular, single stranded RNA molecules that infect plants. Since they non-coding, their structures play a critical role in life cycles. To date, little effort has been spend on elucidating viroid solution due to both the experimental difficulties and time-consuming nature of methodologies implicated. Recently, technique high-throughput selective 2′-hydroxyl acylation analyzed by primer extension (SHAPE) was adapted for probing members family Avsunviroidae, all whom...
G-quadruplexes are widespread four-stranded structures that adopted by G-rich regions of both DNA and RNA involved in essential biological processes such as mRNA translation. They formed the stacking two or more G-quartets linked together three loops. Although maximal loop length is usually fixed to 7 nt most G-quadruplex-predicting software, it has already been demonstrated artificial containing distal loops limited 1 each a central up 30 long likely form vitro. This report demonstrates...
RNA G-Quadruplexes (G4) have been shown to possess many biological functions, including the regulation of microRNA (miRNA) biogenesis and function. However, their impact on pri-miRNA processing remains unknown. We identified G4 located near Drosha cleavage site in three distinct pri-miRNAs: pri-mir200c, pri-mir451a, pri-mir497. The folding potential motifs was determined solution. Subsequently, mutations disrupting led important changes mature miRNAs levels cells. Moreover, using small...
Most human messenger RNAs (mRNAs) are alternatively spliced and many exhibit disease-specific splicing patterns. However, the contribution of most events to development maintenance diseases remains unclear. As alternative diagnosis prognosis is becoming increasingly recognized, it becomes important develop precise methods quantify abundance these isoforms in clinical samples. Here we present a pipeline for real-time PCR annotation (RASE) that allows accurate identification large number...
Prostate cancer remains the single most prevalent in men. Standard therapies are still limited and include androgen ablation that initially causes tumor regression. However, cells eventually relapse develop into a hormone-refractory prostate cancer. One of current challenges this disease is to define new therapeutic targets, which have been virtually unchanged past 30 years. Recent studies suggested family enzymes known as proprotein convertases (PCs) involved various types cancers their...
Dysregulations in alternative splicing (AS) patterns have been associated with many human diseases including cancer. In the present study, alterations to global RNA landscape of cellular genes were investigated a large-scale screen from 377 liver tissue samples using high-throughput sequencing data. Our study identifies modifications AS transcripts encoded by more than 2500 such as tumor suppressor genes, transcription factors, and kinases. These findings provide insights into molecular...
Pre-mRNA alternative splicing is modified in cancer, but the origin and specificity of these changes remain unclear. Here, we probed ovarian tumors to identify cancer-associated isoforms define mechanism by which cancer cells. Using high-throughput quantitative PCR, monitored expression splice variants laser-dissected tissues from tumors. Surprisingly, were not limited tumor also found microenvironment. Changes tumor-associated events be regulated factors that are differentially expressed...
Alternative splicing (AS) is a central mechanism of genetic regulation which modifies the sequence RNA transcripts in higher eukaryotes. AS has been shown to increase both variability and diversity cellular proteome by changing composition resulting proteins through differential choice exons be included mature mRNAs. In present study, alterations global landscape genes upon viral infection were investigated using mammalian reovirus as model. Our study provides first comprehensive portrait...
Abstract It is well established that viroid derived small RNA (vd-sRNA) induces silencing of endogenous mRNA. However, it remains not clear how exactly infections can lead to severe symptom induction given the fact fewer vd-sRNAs binding specific target mRNAs were recovered from infected plants. To answer this question, two least expressed (+) and (−) strand potato spindle tuber (PSTVd) both 3′ UTR coding region tomato analyzed by infecting plants with variants PSTVd. As products these...
Abstract Background It has been observed that following viroid infection, there is an accumulation of viroid-derived siRNAs in infected plants. Some experimental results suggest these small RNAs may be produced by the plant defense system to protect it from indicating viroids can elicit RNA-silencing pathways. The objective this study identify peach latent mosaic (PLMVd), a model RNA genome, regions are most susceptible interference machinery. Results isolated tree have used sequence...
G-quadruplexes (G4) are tetrahelical structures formed from planar arrangement of guanines in nucleic acids. A simple, regular motif was originally proposed to describe G4-forming sequences. More recently, however, formation G4 discovered depend, at least part, on the contextual backdrop neighboring Prediction folding is thus becoming more challenging as outlier structures, not described by motif, increasingly reported. Recent observations call for a comprehensive tool, capable consolidating...
Viroids are small single-stranded RNA pathogens which cause significant damage to plants. As their nucleic acids do not encode for any proteins, they dependant solely on structure propagation. The elucidation of the secondary structures viroids has been limited because exhaustive and time consuming nature classic approaches. Here, method high-throughput selective 2'-hydroxyl acylation analysed by primer extension (hSHAPE) adapted probe viroid structure. data obtained using this were then...