A5029538974- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Chromatography
- Advanced Proteomics Techniques and Applications
- Advancements in Transdermal Drug Delivery
- Molecular Biology Techniques and Applications
- Microfluidic and Capillary Electrophoresis Applications
- Nail Diseases and Treatments
- Bee Products Chemical Analysis
- Protease and Inhibitor Mechanisms
- Pesticide Residue Analysis and Safety
- Cutaneous lymphoproliferative disorders research
- Toxin Mechanisms and Immunotoxins
- Plant Pathogens and Fungal Diseases
- Spectroscopy Techniques in Biomedical and Chemical Research
- Medical Imaging Techniques and Applications
- Fungal Infections and Studies
- Dermatology and Skin Diseases
- Echinoderm biology and ecology
- Marine Sponges and Natural Products
- Immunotoxicology and immune responses
- Glaucoma and retinal disorders
- Nuclear Physics and Applications
- Drug-Induced Ocular Toxicity
- Corneal surgery and disorders
Eurasanté
2012-2024
Paris Biotech Santé
2014
Université de Sherbrooke
2008-2011
Protéomique, Réponse Inflammatoire et Spectrométrie de Masse
2011
Université Lille Nord de France
2010
Université de Lille
2008-2009
Centre National de la Recherche Scientifique
2008
Laboratoire Paul Painlevé
2008
A common technique for the long-term storage of tissues in hospitals and clinical laboratories is preservation formalin-fixed paraffin-embedded (FFPE) blocks. Such stored more than five years have not been useful proteomic studies focused on biomarker discovery. Recently, MS-based analyses FFPE showed positive results blocks less 2 days. However, most samples are one year, thus our objective was to establish a novel strategy using as model system 6-hydroxydopamine (6-OHDA) treated rat brain...
Prostate cancer remains the single most prevalent in men. Standard therapies are still limited and include androgen ablation that initially causes tumor regression. However, cells eventually relapse develop into a hormone-refractory prostate cancer. One of current challenges this disease is to define new therapeutic targets, which have been virtually unchanged past 30 years. Recent studies suggested family enzymes known as proprotein convertases (PCs) involved various types cancers their...
Background Several species of sea cucumbers the family Holothuriidae possess a particular mechanical defense system called Cuvierian tubules (Ct). It is also chemical as triterpene glycosides (saponins) appear to be particularly concentrated in Ct. In present study, precise localization saponins Ct Holothuria forskali investigated. Classical histochemical labeling using lectin was firstly performed but did not generate any conclusive results. Thus, MALDI mass spectrometry Imaging (MALDI-MSI)...
Previously, MS was often used to analyze the composition and structure of biological molecules present in solutions. Today, technology developments enable application for analysis localized biomolecules on tissue surfaces. This technique is called imaging. MALDI imaging a whereby thousands compounds section are detected simultaneously without labeling. Although initially detection such as peptides proteins, this also today drug detection. These characteristics make an ideal that perfectly...
Available imaging systems for use in preclinical toxicology studies increasingly show utility as important tools the toxicologic pathologist's armamentarium, permit longitudinal evaluation of functional and morphological changes tissues, provide information such organ lesion volume not obtained by conventional study parameters. Representative examples practical applications research are presented ultrasound, positron emission tomography/single-photon computed tomography, optical, magnetic...
Keratinocyte organization and biochemistry are important in forming the skin's protective barrier. Intrinsic extrinsic factors can affect skin barrier function at cellular molecular levels. Matrix-assisted laser desorption/ionization (MALDI) mass spectrometric imaging, a technique which combines both aspects histological details, has proven to be valuable method various disciplines including pharmacology, dermatology cosmetology. It typically requires ex vivo samples, prepared following...
Amorolfine 5% lacquer is an established topical treatment for fungal infection of the nails. The success therapy onychomycosis depends on whether permeated drug concentration in deep nail bed retained above effective antifungal minimum inhibitory (MIC). We compared penetration profile amorolfine and a new formula terbinafine human mycotic toenails using matrix-assisted laser desorption ionization mass spectrometry imaging-Fourier transform ion cyclotron resonance (MALDI-FTICR) imaging. 7.8%...
As tumors are very heterogeneous, investigating the penetration and concentration of an anticancer drug in different histological regions a tumor is key to evaluate efficacy, improve pharmacokinetics/pharmacodynamics (PK/PD) relationship evaluation, confirm adequacy dose regimen. Quantitative mass spectrometry imaging (QMSI) allows for determination tissue distribution drugs, metabolites, biomarkers support quick precise evaluation efficacy safety single experiment. QMSI was applied...
The purpose of this study is to identify and visualize the spatial distribution proteins present in amyloid corneal deposits TGFBI-CD patients using Mass Spectrometry Imaging (MSI) compare it with healthy control cornea. Corneal Dystrophies (CD) constitute a group genetically inherited protein aggregation disorders that affects different layers With accumulated deposition, cornea becomes opaque decreased visual acuity. CD affecting stroma Bowman's membrane, associated mutations transforming...
Mass spectrometry imaging datasets are mostly analyzed in terms of average intensity regions interest. However, biological tissues have different morphologies with several sizes, shapes, and structures. The important information, contained this highly heterogeneous cellular organization, could be hidden by analyzing the intensities. Finding an analytical process morphology would help to find such describe tissue model, support identification biomarkers. This study describes informatics...
Lactic acid is a common active ingredient in many topical skincare products; however, measuring its delivery into the skin challenging due to presence of large level endogenous lactic acid. In this study, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) was used quantitatively and qualitatively measure from range products.
Mycosis fungoides (MF) is a subtype of CTCL with low incidence and high medical need for novel treatments. The objective this randomized, placebo-controlled, double-blinded, first-in-human study was to evaluate safety, efficacy, cutaneous systemic pharmacokinetics (PK) topical bimiralisib in healthy volunteers (HVs) MF patients. In trial, total 6 HVs 19 early-stage patients were treated 2.0% gel and/or placebo. Drug efficacy assessed by the Composite Assessment Index Lesion Severity (CAILS)...
Onychomycosis is a fungal infection of the nails that can be challenging to treat. Here, matrix-assisted laser desorption ionization–Fourier transform ion cyclotron resonance (MALDI-FTICR) imaging was applied quantitative analysis penetration profile antifungal compound, amorolfine, in human mycotic toenails. The amorolfine compared with those three other antifungals, ciclopirox, naftifine, and tioconazole. Antifungal compounds (amorolfine 5% lacquer, ciclopirox 8% naftifine 1% solution,...