A5049691691- Plant Molecular Biology Research
- Plant-Microbe Interactions and Immunity
- Seed Germination and Physiology
- Plant Pathogenic Bacteria Studies
- Plant Stress Responses and Tolerance
- Plant Virus Research Studies
- Plant Pathogens and Fungal Diseases
- Plant Genetic and Mutation Studies
- Plant Reproductive Biology
- Bioactive Compounds and Antitumor Agents
- Postharvest Quality and Shelf Life Management
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Synthesis of Organic Compounds
- Plant nutrient uptake and metabolism
- Plant Physiology and Cultivation Studies
- Plant Gene Expression Analysis
- Plant Parasitism and Resistance
- Plant biochemistry and biosynthesis
- Insect-Plant Interactions and Control
- Legume Nitrogen Fixing Symbiosis
- Plant Disease Resistance and Genetics
- Phytoplasmas and Hemiptera pathogens
- Autoimmune and Inflammatory Disorders
- RNA regulation and disease
Universitat Politècnica de València
2016-2025
Instituto de Biología Molecular y Celular de Plantas
2015-2025
Consejo Superior de Investigaciones Científicas
2011-2023
Pasadena City College
2023
Instituto de Biología Molecular y Celular de Rosario
2012-2014
Consejo Nacional de Investigaciones Científicas y Técnicas
2012-2014
National University of Rosario
2012-2014
National University of the Littoral
2014
Instituto de Química del Noroeste Argentino
2012-2014
Hospital César Milstein
2012-2014
Abstract Plants use volatile terpene compounds as odor cues for communicating with the environment. Fleshy fruits are particularly rich in volatiles that deter herbivores and attract seed dispersal agents. We have investigated how terpenes citrus fruit peels affect interaction between plant, insects, microorganisms. Because limonene represents up to 97% of total orange (Citrus sinensis) peel, we chose down-regulate expression a synthase gene plants by introducing an antisense construct this...
Pathogenesis-related (PR) proteins form a heterogeneous family of plant that are likely to be involved in defense and inducible by pathogen attacks. One group PRs, represented the subfamily PR-1, low-molecular-weight unknown biochemical function. Here we describe cloning characterization two closely related genes encoding basic an acidic PR-1 protein (PR1b1 PR1a2) from tomato (Lycopersicon esculentum). We present comparative study mode transcriptional regulation these transgenic tobacco...
Abstract Seed longevity is a polygenic trait of relevance for agriculture and understanding the effect environment on ageing biological systems. In order to identify novel genes, we have phenotyped natural variation 270 ecotypes model plant, Arabidopsis thaliana , three accelerated methods. Genome‐wide analysis, using publicly available single‐nucleotide polymorphisms (SNPs) data sets, identified multiple genomic regions associated with in seed longevity. Reverse genetics 20 candidate genes...
The Arabidopsis GNOM protein, a guanine nucleotide exchange factor (GEF) that acts on ADP ribosylation (ARF)–type G proteins, is required for coordination of cell polarity along the apical–basal embryo axis. Interallelic complementation gnom mutants suggested dimerization involved in function. Here, direct interaction between molecules demonstrated vitro and by using yeast two-hybrid system. Interaction was confined to an N-terminal domain conserved within subgroup large ARF GEFs. same...
Abstract Terpenoid volatiles are isoprene compounds that emitted by plants to communicate with the environment. In addition their function in repelling herbivores and attracting carnivorous predators green tissues, presumed primary of terpenoid released from mature fruits is attraction seed-dispersing animals. Mature oranges (Citrus sinensis) primarily accumulate terpenes peel oil glands, d-limonene accounting for approximately 97% total volatile terpenes. a previous report, we showed...
Penicillium spp. are the major postharvest pathogens of citrus fruit in Mediterranean climatic regions. The induction natural resistance constitutes one most promising alternatives to avoid environmental contamination and health problems caused by chemical fungicides. To understand bases against digitatum, we have used a 12k cDNA microarray study transcriptional changes outer inner parts peel (flavedo albedo, respectively) elicited fruits. elicitor treatment led an over-representation...
The increased selection pressure of the herbicide glyphosate has played a role in evolution glyphosate-resistance weedy species, an issue that is becoming threat to global agriculture. molecular components involved cellular toxicity response this at expression level are still unidentified.
Understanding the nature of pathogen host interaction may help improve strawberry (Fragaria × ananassa) cultivars. Plant resistance to pathogenic agents usually operates through a complex network defense mechanisms mediated by diverse array signaling molecules. In strawberry, variety pathogens has been reported be mostly polygenic and quantitatively inherited, making it difficult associate molecular markers with disease genes. Colletotrichum acutatum spp. is major pathogen, completely...
Abstract The construction of communication models at the micro‐/nanoscale involving abiotic nanodevices and living organisms has potential to open a wide range applications in biomedical technologies. However, this area remains almost unexplored. Herein, we report, as proof concept, stimuli‐responsive interactive paradigm between yeasts (as model microorganism) enzyme‐controlled Janus Au–mesoporous silica nanoparticles. In presence stimulus, information flows from microorganism nanodevice,...
Permeability is a crucial trait that affects seed longevity and regulated by different polymers including proanthocyanidins, suberin, cutin lignin located in the coat. By testing mutants suberin transport biosynthesis, we demonstrate importance of this biopolymer to cope with deterioration. Transcriptomic analysis cog1-2D, gain-of-function mutant increased longevity, revealed upregulation several peroxidase genes. Reverse genetics analysing uncovered redundancy within coat gene family;...
Huanglongbing (HLB) is a devastating disease of citrus plants caused by the non-culturable phloem-inhabiting bacterium Candidatus Liberibacter ssp., being Ca. asiaticus (CLas) most aggressive species. CLas vectored psyllid Diaphorina citri and introduced into sieve cells, establishing successful infection in all Citrus Partial or complete resistance has been documented distant relatives Murraya paniculata Bergera koenigii, respectively, providing excellent systems to investigate molecular...
Abstract To gain further insight into abscisic acid (ABA) signaling and its role in growth regulation, we have screened for Arabidopsis (Arabidopsis thaliana) mutants hypersensitive to ABA-mediated root inhibition. As a result, identified loss-of-function allele of BREVIS RADIX (BRX) the Columbia background, named brx-2, which shows enhanced response inhibition growth. BRX encodes key regulator cell proliferation elongation root, has been implicated brassinosteroid (BR) pathway as well...
Summary Seeds slowly accumulate damage during storage, which ultimately results in germination failure. The seed coat protects the embryo from external environment, and its composition is critical for longevity. Flavonols outer integument. link between flavonol integument development has not been explored. Genetic, molecular ultrastructural assays on loss‐of‐function mutants of flavonoid biosynthesis pathway were used to study effect altered Controlled deterioration indicate that loss...
Differential hybridization was used to isolate genes induced by viroid infection in tomato plants. Four new cDNA clones encoding a peroxidase, desaturase-like enzyme, lipoxygenase, and proteinase inhibitor, were selected characterized. All of these display characteristic expression pattern, showing constitutive roots healthy plants being ectopically activated aerial tissues upon ethephon treatment. Possible functions for the viroid-tomato interaction are proposed. The existence an integrated...
Accurate DNA replication is one of the most important events in life a cell. To perform this task, cell utilizes several polymerase complexes. We investigated role epsilon during gametophyte and seed development using forward reverse genetic approaches. In Arabidopsis, catalytic subunit complex encoded by two genes, AtPOL2a AtPOL2b, whereas second largest regulatory AtDPB2 present as unique complete copy. Disruption or resulted sporophytic embryo-defective phenotype, whilst mutations AtPOL2b...
Understanding of genetic elements that contribute to key aspects citrus biology will impact future improvements in this economically important crop. Global gene expression analysis demands microarray platforms with a high genome coverage. In the last years, genome-wide EST collections have been generated citrus, opening possibility create new tools for functional genomics crop plant.We designed and constructed publicly available cDNA include 21,081 putative unigenes citrus. As companion...
The effect of gibberellins (GA) on internode transcriptome was investigated in transgenic Carrizo citrange (Citrus sinensis x Poncirus trifoliata) plants overexpressing endogenous CcGA20ox1 (encoding a GA biosynthetic gene), and non-transformed explants treated with GA(3), using citrus cDNA microarray. Substantial modulation gene expression found sense CcGA20ox plants. Extensive up-regulation genes involved photosynthesis carbon utilization, down-regulation those protein synthesis ribosome...
In recent years, class prediction experiments have been largely developed in cancer research with the aim of classifying unknown samples by examining their expression signature. natural populations, a significant component gene variability is also heritable. Citrus species are an ideal model to accomplish study these questions plants, due existence varieties derived from somatic mutations that likely differ each other one or few point but phenotypically indistinguishable at early vegetative...
Re-activation of cell division after fertilization involves the specific regulation a set genes. To identify genes involved in gametophytic to sporophytic transition, we screened Arabidopsis T-DNA insertion lines for early seed abortion at zygote (zeus) or one-cell embryo stages (cyclops), and characterized zygote-lethal mutation, zeus1. ZEUS1 encodes thymidylate kinase (AtTMPK) that synthesizes dTDP is DNA replication. Unlike yeast animals, single AtTMPK gene capable producing two proteins...
Plant volatiles include terpenoids, which are generally involved in plant defense, repelling pests and pathogens attracting insects for herbivore control, pollination seed dispersal. Orange fruits accumulate the monoterpene limonene at high levels oil glands of their fruit peels. When production was downregulated orange by transgenic expression a synthase (CitMTSE1) antisense configuration, these were resistant to fungus Penicillium digitatum (Pers.) Sacc. bacterium Xanthomonas citri subsp....
Cutin and suberin are lipid polyesters deposited in specific apoplastic compartments. Their fundamental roles plant biology include controlling the movement of gases, water solutes, conferring pathogen resistance. Both cutin have been shown to be present Arabidopsis seed coat where they regulate dormancy longevity. In this study, we use accelerated natural ageing assays, glutathione redox potential measures, optical transmission electron microscopy gas chromatography-mass spectrometry...