This work characterized the role of R2R3-MYB10 transcription factor (TF) in strawberry fruit ripening. The expression this TF takes place mainly receptacle and is repressed by auxins activated abscisic acid (ABA), parallel to ripening process. Anthocyanin was not produced when FaMYB10 transiently silenced receptacles. An increase observed water-stressed fruits, which accompanied an both ABA anthocyanin content. High-throughput transcriptomic analyses performed fruits with downregulated...

Strawberry softening is characterized by an increase in the solubilization and depolymerization of pectins from cell walls. Galactose release pectin side chains β-galactosidase enzymes has been proposed as one reason for soluble pectins. A putative gene, FaβGal4, identified using a custom-made oligonucleotide-based strawberry microarray platform. FaβGal4 was expressed mainly receptacle during fruit ripening, positively regulated abscisic acid negatively auxins. To ascertain role softening,...

Short-chain esters contribute to the blend of volatiles that define strawberry aroma. The last step in their biosynthesis involves an alcohol acyltransferase catalyses esterification acyl moiety acyl-CoA with alcohol. This study identified a novel gene (FaAAT2) whose expression pattern during fruit receptacle growth and ripening is accordance production throughout ripening. full-length FaAAT2 cDNA was cloned expressed Escherichia coli its activity analysed substrates. semi-purified enzyme...

Abstract Eugenol is a volatile phenylpropanoid that contributes to flower and ripe fruit scent. In strawberry (Fragaria × ananassa) receptacles, eugenol biosynthesized by synthase (FaEGS2). However, the transcriptional regulation of this process still unknown. We have identified functionally characterized an R2R3 MYB transcription factor (EMISSION OF BENZENOID II [FaEOBII]) seems be orthologous gene PhEOBII from Petunia hybrida, which biosynthesis in petals. The expression FaEOBII was...

Firmness is one of the most important fruit quality traits in strawberries. The postharvest shelf life this soft highly limited by loss firmness, where cell wall disassembly plays an role. Previous studies demonstrated that polygalacturonase FaPG1 has a key role remodelling pectins during strawberry softening. In study, knockout plants have been generated using CRISPR/Cas9 system delivered via Agrobacterium tumefaciens. Ten independent lines, cv. "Chandler", were obtained, and all them...

Abstract Using cDNA microarrays, a comprehensive investigation of gene expression was carried out in strawberry (Fragaria × ananassa) fruit to understand the flow events associated with its maturation and non-climacteric ripening. We detected key processes novel genes not previously development ripening, related vascular development, oxidative stress, auxin response. Microarray analysis during receptacle seed (achene) tissues established an interesting parallelism between...

A gene encoding a carotenoid cleavage dioxygenase class 1 enzyme (FaCCD1) was identified among strawberry fruit expressed sequence tag collection. The full-length cDNA isolated, and the expression profiles along receptacle development ripening, determined by quantitative real time polymerase chain reaction, showed that FaCCD1 is ripening-related reaches its maximal level of in red fully ripe stage. Escherichia coli, products formed recombinant protein through oxidative carotenoids were...

Abstract Plant phenolics have drawn increasing attention due to their potential nutritional benefits. Although the basic reactions of biosynthetic pathways in plants been intensively analyzed, regulation accumulation and flux through pathway is not that well established. The aim this study was use a strawberry (Fragaria × ananassa) microarray investigate gene expression patterns associated with phenylpropanoids, flavonoids, anthocyanins fruit. An examination transcriptome, coupled metabolite...

Summary Late blight caused by the plant pathogenic oomycete Phytophthora infestans is known as one of most destructive potato diseases. Plant breeders tend to employ NB ‐ LRR ‐based resistance for introducing genetically controlled late in their breeding lines. However, P. able rapidly escape this type resistance, and hence, cultivars often not durable. Previously, we identified a novel Arabidopsis. This mediated cell surface receptor Lec RK ‐I.9, which belongs family L‐type lectin kinases....

Pectins are essential components of primary plant cell walls and middle lamellae, related to the consistency fruit its textural changes during ripening. In fact, strawberries become soft as lamellae cortical parenchyma cells extensively degraded ripening, leading observed short post-harvest shelf life. Using a custom-made oligonucleotide-based strawberry microarray platform, putative rhamnogalacturonate lyase gene (FaRGlyase1) was identified. Bioinformatic analysis FaRGlyase1 sequence...

Antisense-mediated down-regulation of the fruit-specific polygalacturonase (PG) gene FaPG1 in strawberries (Fragaria×ananassa Duch.) has been previously demonstrated to reduce fruit softening and extend post-harvest shelf life, despite low PG activity detected this fruit. The improved traits were suggested be attributable a reduced cell wall disassembly due silencing. This research provides empirical evidence that supports assumption at biochemical, cellular, tissue levels. Cell...

Cultivated strawberry is a hybrid octoploid species (Fragaria xananassa Duchesne ex. Rozier) whose fruit highly appreciated due to its organoleptic properties and health benefits. Despite recent studies on the control of growth ripening processes, information about role played by different hormones these processes remains elusive. Further advancement this knowledge hampered limited sequence genes from species, despite abundant available wild diploid relative Fragaria vesca. However, or one...

Strawberry (Fragaria spp) is an emerging model for the development of basic genomics and recombinant DNA studies among rosaceous crops. Functional genomic molecular involve relative quantification gene expression under experimental conditions interest. Accuracy reliability are dependent upon choice optimal reference control transcript. There no information available on validated endogenous genes use in testing strawberry-pathogen interactions. Thirteen potential pre-selected strawberry were...

To disentangle the role of polygalacturonase (PG) genes in strawberry softening, two PG most expressed ripe receptacles, FaPG1 and FaPG2, were down-regulated. Transgenic fruits firmer than those wild type when silenced individually. Simultaneous silencing both by transgene stacking did not result an additional increase firmness. Cell walls from characterized a carbohydrate microarray. Higher signals homogalacturonan rhamnogalacturonan I pectin epitopes polysaccharide fractions tightly bound...

In this work, we identified and functionally characterized the strawberry (Fragaria × ananassa) R2R3 MYB transcription factor FaMYB123. As in most genes associated with organoleptic properties of ripe fruit, FaMYB123 expression is ripening-related, receptacle-specific, antagonistically regulated by ABA auxin. Knockdown RNAi fruit receptacles downregulated enzymes involved late steps anthocyanin/flavonoid biosynthesis. Transgenic fruits showed a parallel decrease contents total anthocyanin...

Two genomic clones corresponding to putative pectate lyase genes (plA and plB) were isolated characterized in strawberry (Fragaria×ananassa cv. Chandler). The ORFs for the plA plB revealed deduced proteins of 451 439 amino acids, respectively, that differ from previously plC gene. Southern blot analysis has shown while gene is a single copy gene, probably encoded by small multigene family. By using specific probes untranslated 3′ terminal region pl genes, QRT‐PCR methodology, spatio‐temporal...

Numerous GAST-like genes have been reported in higher plants, but only one gene (FaGAST1) has described strawberry so far. Herein, we identified a novel FaGAST (FaGAST2) whose expression showed an increase throughout fruit receptacle development and ripening, coinciding with those stages where decrease expansion processes (G3–W R–OR stages) occurs. FaGAST2 shares 31% 15.7% amino acid nucleotide sequence homology, respectively, the previously FaGAST1 gene, both contain signal peptide highly...

Understanding the nature of pathogen host interaction may help improve strawberry (Fragaria × ananassa) cultivars. Plant resistance to pathogenic agents usually operates through a complex network defense mechanisms mediated by diverse array signaling molecules. In strawberry, variety pathogens has been reported be mostly polygenic and quantitatively inherited, making it difficult associate molecular markers with disease genes. Colletotrichum acutatum spp. is major pathogen, completely...

Only a few transcription factors have been described in the regulation of strawberry (Fragaria x ananassa) fruit ripening process. Using transcriptomic approach, we identified and functionally characterized FaDOF2, DOF-type ripening-related factor, which is hormonally regulated specific to receptacle, though high expression levels were also found petals. The pattern FaDOF2 correlated with eugenol content, phenylpropanoid volatile, both receptacles When was silenced ripe receptacles, FaEOBII...

NAC proteins are a family of transcription factors which have variety important regulatory roles in plants. They present very well conserved group subdomains the N-terminal region and highly variable domain at C-terminus. Currently, knowledge concerning strawberry plant remains limited. In this work, we analyzed Fragaria vesca, total 112 were identified after curated annotations from version 4.0.a1 genome. placed into ligation groups (pseudo-chromosomes) described its physicochemical genetic...

Host resistance is the most effective and practical control method for management of Verticillium wilt in olive caused by dahliae, which remains as one major current threats to this crop. Regrettably, cultivars agronomic commercial interest are susceptible V. dahliae. We previously demonstrated that wild (Olea europaea L. subsp. var. sylvestris) clone AC18 harbours highly virulent defoliating (D) dahliae pathotype, may be valuable rootstock breeding new, resistant cultivars. Mechanisms...

Cinnamyl alcohol dehydrogenase (CAD; EC 1.1.1.195) catalyses the conversion of p‐hydroxy‐cinnamaldehydes to corresponding alcohols and is considered a key enzyme in lignin biosynthesis. By differential screening strawberry (Fragaria× ananassa cv. Chandler) fruit specific subtractive cDNA library, full‐length clone cad gene was isolated (Fxacad1). Northern blot quantitative real time PCR studies indicated that Fxacad1 expressed fruits, runners, leaves, flowers but not roots. In addition,...