A5089442000- Plant Gene Expression Analysis
- Berry genetics and cultivation research
- Plant tissue culture and regeneration
- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Horticultural and Viticultural Research
- Plant Physiology and Cultivation Studies
- Postharvest Quality and Shelf Life Management
- Plant Reproductive Biology
- Plant Molecular Biology Research
- Polysaccharides and Plant Cell Walls
- Plant biochemistry and biosynthesis
- Plant Pathogenic Bacteria Studies
- Plant Stress Responses and Tolerance
- Garlic and Onion Studies
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Phytochemicals and Antioxidant Activities
- Transgenic Plants and Applications
- Plant pathogens and resistance mechanisms
- Microbial Natural Products and Biosynthesis
- Edible Oils Quality and Analysis
- Molecular Biology Techniques and Applications
- Mobile Agent-Based Network Management
- Network Time Synchronization Technologies
University of Córdoba
2016-2025
Honduras Foundation for Agricultural Research
2025
Cordoba University
2022
Andalusian Institute of Agricultural and Fisheries Research and Training
2003-2012
Universidad de Málaga
2009
Zero to Three
2009
Technical University of Munich
2008
Universidad de La Laguna
2003
Weatherford College
1994
657 Oslo
1994
This work characterized the role of R2R3-MYB10 transcription factor (TF) in strawberry fruit ripening. The expression this TF takes place mainly receptacle and is repressed by auxins activated abscisic acid (ABA), parallel to ripening process. Anthocyanin was not produced when FaMYB10 transiently silenced receptacles. An increase observed water-stressed fruits, which accompanied an both ABA anthocyanin content. High-throughput transcriptomic analyses performed fruits with downregulated...
Strawberry (Fragaria x ananassa, Duch., cv Chandler) is a soft fruit with short postharvest life, mainly due to rapid lost of firm texture. To control the strawberry softening, we obtained transgenic plants that incorporate an antisense sequence pectate lyase gene under 35S promoter. Forty-one independent lines (Apel lines) were obtained, propagated in greenhouse for agronomical analysis, and compared plants, non-transformed transformed pGUSINT plasmid. Total yield was significantly reduced...
The flavor of strawberry (Fragaria x ananassa) fruit is dominated by an uncommon group aroma compounds with a 2,5-dimethyl-3(H)-furanone structure. We report the characterization enzyme involved in biosynthesis 4-hydroxy-2,5-dimethyl-3(2H)-furanone (HDMF; Furaneol), key compound strawberries. Protein extracts were partially purified, and observed distribution enzymatic activity correlated presence single polypeptide approximately 37 kD. Sequence analysis two peptide fragments showed total...
Knowledge of the molecular basis plant resistance to pathogens in species other than Arabidopsis is limited. The function Fa WRKY1, first WRKY gene isolated from strawberry (Fragaria x ananassa), an important agronomical fruit crop, has been investigated here. WRKY1 encodes a IIc transcription factor and up-regulated following Colletotrichum acutatum infection, treatments with elicitors, wounding. Its sequence homologue, At WRKY75, described as playing role regulating phosphate starvation...
Short-chain esters contribute to the blend of volatiles that define strawberry aroma. The last step in their biosynthesis involves an alcohol acyltransferase catalyses esterification acyl moiety acyl-CoA with alcohol. This study identified a novel gene (FaAAT2) whose expression pattern during fruit receptacle growth and ripening is accordance production throughout ripening. full-length FaAAT2 cDNA was cloned expressed Escherichia coli its activity analysed substrates. semi-purified enzyme...
Abstract Eugenol is a volatile phenylpropanoid that contributes to flower and ripe fruit scent. In strawberry (Fragaria × ananassa) receptacles, eugenol biosynthesized by synthase (FaEGS2). However, the transcriptional regulation of this process still unknown. We have identified functionally characterized an R2R3 MYB transcription factor (EMISSION OF BENZENOID II [FaEOBII]) seems be orthologous gene PhEOBII from Petunia hybrida, which biosynthesis in petals. The expression FaEOBII was...
Food-baited traps are an important part of early detection programs for invasive tephritid fruit fly species, as they attractive to both sexes all targeted species. Torula yeast borax (TYB) mixture is a standard food bait, but its longevity limited (1–2 weeks). Synthetic food-based lures have been developed, including ammonium acetate, putrescine, and trimethylamine. However, the different formulations these synthetic vary greatly in their attractiveness longevity. Here, we present results...
Rubbish dumps provide an extra and constant food source for many birds. The White Stork (Ciconia ciconia) is one of the species that have taken advantage these new foraging areas both during breeding season winter. This study analyzes data collected in Spanish province Cordoba throughout five years between 1992 1998 on biology success influence rubbish dumps. Breeding varied significantly two years. A banding program from 1990 to resulted 145 storks being marked as nestlings. Of these, 49...
A gene encoding a carotenoid cleavage dioxygenase class 1 enzyme (FaCCD1) was identified among strawberry fruit expressed sequence tag collection. The full-length cDNA isolated, and the expression profiles along receptacle development ripening, determined by quantitative real time polymerase chain reaction, showed that FaCCD1 is ripening-related reaches its maximal level of in red fully ripe stage. Escherichia coli, products formed recombinant protein through oxidative carotenoids were...
Abstract Plant phenolics have drawn increasing attention due to their potential nutritional benefits. Although the basic reactions of biosynthetic pathways in plants been intensively analyzed, regulation accumulation and flux through pathway is not that well established. The aim this study was use a strawberry (Fragaria × ananassa) microarray investigate gene expression patterns associated with phenylpropanoids, flavonoids, anthocyanins fruit. An examination transcriptome, coupled metabolite...
Pectins are essential components of primary plant cell walls and middle lamellae, related to the consistency fruit its textural changes during ripening. In fact, strawberries become soft as lamellae cortical parenchyma cells extensively degraded ripening, leading observed short post-harvest shelf life. Using a custom-made oligonucleotide-based strawberry microarray platform, putative rhamnogalacturonate lyase gene (FaRGlyase1) was identified. Bioinformatic analysis FaRGlyase1 sequence...
Cultivated strawberry is a hybrid octoploid species (Fragaria xananassa Duchesne ex. Rozier) whose fruit highly appreciated due to its organoleptic properties and health benefits. Despite recent studies on the control of growth ripening processes, information about role played by different hormones these processes remains elusive. Further advancement this knowledge hampered limited sequence genes from species, despite abundant available wild diploid relative Fragaria vesca. However, or one...
Strawberry (Fragaria spp) is an emerging model for the development of basic genomics and recombinant DNA studies among rosaceous crops. Functional genomic molecular involve relative quantification gene expression under experimental conditions interest. Accuracy reliability are dependent upon choice optimal reference control transcript. There no information available on validated endogenous genes use in testing strawberry-pathogen interactions. Thirteen potential pre-selected strawberry were...
In this work, we identified and functionally characterized the strawberry (Fragaria × ananassa) R2R3 MYB transcription factor FaMYB123. As in most genes associated with organoleptic properties of ripe fruit, FaMYB123 expression is ripening-related, receptacle-specific, antagonistically regulated by ABA auxin. Knockdown RNAi fruit receptacles downregulated enzymes involved late steps anthocyanin/flavonoid biosynthesis. Transgenic fruits showed a parallel decrease contents total anthocyanin...
Two genomic clones corresponding to putative pectate lyase genes (plA and plB) were isolated characterized in strawberry (Fragaria×ananassa cv. Chandler). The ORFs for the plA plB revealed deduced proteins of 451 439 amino acids, respectively, that differ from previously plC gene. Southern blot analysis has shown while gene is a single copy gene, probably encoded by small multigene family. By using specific probes untranslated 3′ terminal region pl genes, QRT‐PCR methodology, spatio‐temporal...
Six open reading frames (ORFs) were identified by DNA sequencing of 5.7 kilobase pairs at the left end act cluster (the so-called actVI region), in order: ORFB, ORFA, ORF1, ORF2, ORF3, ORF4. ORF1-4 are transcribed rightward and same direction as ORFs actVA region which lies to right region, whereas ORFA ORFB run opposite direction. By complementation mutants gene disruption wild type strain, two previously genetically characterized mutations assigned ORF1. Although ORF4, using phi C31...
Numerous GAST-like genes have been reported in higher plants, but only one gene (FaGAST1) has described strawberry so far. Herein, we identified a novel FaGAST (FaGAST2) whose expression showed an increase throughout fruit receptacle development and ripening, coinciding with those stages where decrease expansion processes (G3–W R–OR stages) occurs. FaGAST2 shares 31% 15.7% amino acid nucleotide sequence homology, respectively, the previously FaGAST1 gene, both contain signal peptide highly...