A5047545093- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Plant Gene Expression Analysis
- Berry genetics and cultivation research
- Molecular Biology Techniques and Applications
- bioluminescence and chemiluminescence research
- Plant Pathogenic Bacteria Studies
- Advanced Proteomics Techniques and Applications
- Microbial Community Ecology and Physiology
- Algal biology and biofuel production
- Plant Stress Responses and Tolerance
- Postharvest Quality and Shelf Life Management
- Protein Hydrolysis and Bioactive Peptides
- Plant pathogens and resistance mechanisms
- Genetic Mapping and Diversity in Plants and Animals
- Plant Disease Management Techniques
- Plant tissue culture and regeneration
- Wheat and Barley Genetics and Pathology
- Horticultural and Viticultural Research
- Genomics and Phylogenetic Studies
- Fungal and yeast genetics research
- Plant responses to elevated CO2
- Plant and Fungal Interactions Research
- Marine Biology and Environmental Chemistry
- Probiotics and Fermented Foods
University of Córdoba
2011-2024
This work characterized the role of R2R3-MYB10 transcription factor (TF) in strawberry fruit ripening. The expression this TF takes place mainly receptacle and is repressed by auxins activated abscisic acid (ABA), parallel to ripening process. Anthocyanin was not produced when FaMYB10 transiently silenced receptacles. An increase observed water-stressed fruits, which accompanied an both ABA anthocyanin content. High-throughput transcriptomic analyses performed fruits with downregulated...
Knowledge of the molecular basis plant resistance to pathogens in species other than Arabidopsis is limited. The function Fa WRKY1, first WRKY gene isolated from strawberry (Fragaria x ananassa), an important agronomical fruit crop, has been investigated here. WRKY1 encodes a IIc transcription factor and up-regulated following Colletotrichum acutatum infection, treatments with elicitors, wounding. Its sequence homologue, At WRKY75, described as playing role regulating phosphate starvation...
Strawberry (Fragaria spp) is an emerging model for the development of basic genomics and recombinant DNA studies among rosaceous crops. Functional genomic molecular involve relative quantification gene expression under experimental conditions interest. Accuracy reliability are dependent upon choice optimal reference control transcript. There no information available on validated endogenous genes use in testing strawberry-pathogen interactions. Thirteen potential pre-selected strawberry were...
Numerous GAST-like genes have been reported in higher plants, but only one gene (FaGAST1) has described strawberry so far. Herein, we identified a novel FaGAST (FaGAST2) whose expression showed an increase throughout fruit receptacle development and ripening, coinciding with those stages where decrease expansion processes (G3–W R–OR stages) occurs. FaGAST2 shares 31% 15.7% amino acid nucleotide sequence homology, respectively, the previously FaGAST1 gene, both contain signal peptide highly...
Understanding the nature of pathogen host interaction may help improve strawberry (Fragaria × ananassa) cultivars. Plant resistance to pathogenic agents usually operates through a complex network defense mechanisms mediated by diverse array signaling molecules. In strawberry, variety pathogens has been reported be mostly polygenic and quantitatively inherited, making it difficult associate molecular markers with disease genes. Colletotrichum acutatum spp. is major pathogen, completely...
Important losses in strawberry production are caused by species of the fungus Colletotrichum , causal agent anthracnose. However, very limited studies at molecular level exist mechanisms related to susceptibility against this pathogen. We have analysed a moderately resistant cultivar (cv. Andana) together with susceptible one Camarosa) during process infection acutatum level. To gain insight into interaction we identified large number genes involved signalling, transcriptional control,...
Wheat can adapt to most agricultural conditions across temperate regions. This success is the result of phenotypic plasticity conferred by a large and complex genome composed three homoeologous genomes (A, B, D). Although drought major cause yield quality loss in wheat, adaptive mechanisms gene networks underlying responses field remain largely unknown. Here, we addressed this utilizing an interdisciplinary approach involving water status phenotyping, sampling, expression analyses. Overall,...
Strawberry (Fragaria x ananassa) is a major food crop worldwide, due to the flavor, aroma and health benefits of fruit, but its productivity quality are seriously limited by large variety phytopathogens, including Colletotrichum spp. So far, key factors regulating strawberry immune response remain unknown. The FaWRKY1 gene has been previously proposed as an important element mediating defense responses in C. acutatum. To get further insight into functional role that plays mechanism,...
Transcriptome analysis is widely used in plant biology research to explore gene expression across a large variety of biological contexts such as those related environmental stress and plant-pathogen interaction. Currently, next generation sequencing platforms are obtain high amount raw data build the transcriptome any plant. Here, we compare Illumina Ion Torrent for construction holm oak (Quercus ilex) transcriptome. Genomic this forest tree species major challenge considering its...
Common bean (Phaseolus vulgaris L.), one of the most important legume crops, uses atmospheric nitrogen through symbiosis with soil rhizobia, reducing need for fertilization. However, this is particularly sensitive to drought conditions, prevalent in arid regions where crop cultured. Therefore, studying response sustain productivity. We have used integrated transcriptomic and metabolomic analysis understand molecular responses water deficit a marker-class common accession cultivated under N2...
DATA REPORT article Front. Mol. Biosci., 06 October 2017 | https://doi.org/10.3389/fmolb.2017.00070
WRKY transcription factors play critical roles in plant growth and development or stress responses. Using up-to-date genomic data, a total of 64 257 genes have been identified the diploid woodland strawberry, Fragaria vesca, more complex allo-octoploid commercial × ananassa cv. Camarosa, respectively. The completeness new genomes annotations has enabled us to perform detailed evolutionary functional study strawberry family members, particularly case cultivated hybrid, which homoeologous...
Some polyploid species show enhanced physiological tolerance to drought compared with their progenitors. However, very few studies have examined the consistency of response between genetically differentiated natural populations, which is key evaluation importance adaptive evolution after polyploidization in those systems where exerts a selective pressure.A comparative functional approach was used investigate differentiation drought-tolerance-related traits Brachypodium complex, model system...
Abstract The plant VQ motif-containing proteins are a recently discovered class of regulatory interacting with WRKY transcription factors capable modulate their activity as transcriptional regulators. short motif (FxxhVQxhTG) is the main element in WRKY-VQ interaction, whereas newly identified variable upstream amino acid appears to be determinant for specificity. family has been studied several species and seems play important roles variety biological processes, including response biotic...
Proteomics is a crucial tool for unravelling the molecular dynamics of essential biological processes, becoming pivotal technique basic and applied research. Diverse bioinformatic tools are required to manage explore huge amount information obtained from single proteomics experiment. Thus, functional annotation protein–protein interactions evaluated in depth leading conclusions that best fit proteomic response system under study. To gain insight into potential applications identified...
The alkaliphilic bacterium Pseudomonas pseudoalcaligenes CECT5344 uses free cyanide and several metal−cyanide complexes as the sole nitrogen source tolerates high concentrations of metals like copper, zinc iron, which are present in jewelry wastewaters. To understand deeply regulatory mechanisms involved transcriptional regulation cyanide-containing wastewaters detoxification by P. CECT5344, RNA-Seq has been performed from cells cultured with a wastewater, sodium or ammonium chloride source....
The improvement of mesophilic biomethanisation recalcitrant sewage sludge derived from urban wastewater treatment through the application a sonication pretreatment was evaluated in parallel two pilot-scale anaerobic digesters (two biological replicates: reactors RA and RB). valorisation process monitored novel holistic approach that related yield, its main batch operational parameters, with abundance archaeal bacterial communities inocula. Sonication allowed achieving methane yield...
Strawberry fruit is highly appreciated worldwide for its organoleptic and healthy properties. However, this plant attacked by many pathogenic fungi, which significantly affect production quality at pre- post-harvest stages, making chemical applications the most effective but undesirable strategy to control diseases, so far. Alternatively, genetic manipulation employing key genes involved in defense, such as members of NPR-like gene family, has been successful crops improve resistance. The...
Abstract Protein phosphorylation and membrane proteins play an important role in the infection of plants by phytopathogenic fungi, given their involvement signal transduction cascades. Botrytis cinerea is a well-studied necrotrophic fungus taken as model organism fungal plant pathology, its broad host range adverse economic impact. To elucidate relevant events during infection, several proteomics analyses have been performed B . , but they cover only 10% total predicted genome database this...