A5011855370- Advanced Proteomics Techniques and Applications
- Redox biology and oxidative stress
- Microbial Community Ecology and Physiology
- Metabolomics and Mass Spectrometry Studies
- Maternal Mental Health During Pregnancy and Postpartum
- Heat shock proteins research
- Adipose Tissue and Metabolism
- bioluminescence and chemiluminescence research
- Mass Spectrometry Techniques and Applications
- Genomics, phytochemicals, and oxidative stress
- Protein Hydrolysis and Bioactive Peptides
- Sulfur Compounds in Biology
- Wastewater Treatment and Nitrogen Removal
- Algal biology and biofuel production
- Antioxidant Activity and Oxidative Stress
- Probiotics and Fermented Foods
- Gut microbiota and health
- Molecular Biology Techniques and Applications
- Fungal Plant Pathogen Control
- Marine Biology and Environmental Chemistry
- Antimicrobial Peptides and Activities
- Advanced Glycation End Products research
- Silk-based biomaterials and applications
- Genetics, Bioinformatics, and Biomedical Research
- Genetic and Environmental Crop Studies
University of Córdoba
2005-2024
Bentham Science Publishers (United Arab Emirates)
2013
Bentham Science Publishers (China)
2013
Denitrification is a respiratory process that produces nitrous oxide as an intermediate, which may escape to the atmosphere before its reduction dinitrogen through reductase NosZ. In this work, denitrification carried out by Paracoccus denitrificans PD1222 has been explored quantitative proteomic analysis. Under anaerobic conditions, with nitrate sole nitrogen source, synthesis of all enzymes involved in denitrification, nitrate, nitrite, nitric and reductases, was increased. However,...
Abstract Denitrification is a respiratory process by which nitrate reduced to dinitrogen. Incomplete denitrification results in the emission of greenhouse gas nitrous oxide and this potentiated acidic soils, display rates high N 2 O/N ratios compared alkaline soils. In work, impact pH on proteome soil denitrifying bacterium Paracoccus denitrificans PD1222 was analysed with as sole energy nitrogen source under anaerobic conditions at ranging from 6.5 7.5. Quantitative proteomic analysis...
Abstract We evaluated the suitability of high‐throughput proteomic methods to monitor terrestrial ecosystems. Free‐living Mus spretus from three sites along “Domingo Rubio” (DR) stream were compared with mice Doñana Biological Reserve (“Santa Olalla” lagoon (SOL) negative control), using specimens an industrial settlement (phosphogypsum stacks (PS)) and rice fields (“Matochal” (ARZ)) as positive controls. Our 2‐DE analysis showed 36 spots significantly altered expression. Sixteen identified...
Abstract Maslinic acid (MA) is a pentacyclic triterpene used as feed additive to stimulate growth, protein‐turnover rates, and hyperplasia in fish. To further our understanding of cellular mechanisms underlying the action MA, we have 2‐DE coupled with MS identify proteins differentially expressed livers juvenile gilthead sea bream ( Sparus aurata ) grown under fish‐farm conditions fed 100 mg/kg MA‐enriched diet (MA ). After comparison protein profiles from MA fish control, 49 spots were...
The aim of the present study was to define role Trx and Grx on metabolic thiol redox regulation identify their protein metabolite targets. hepatocarcinoma-derived HepG2 cell line under both normal oxidative/nitrosative conditions by overexpression NO synthase (NOS3) used as experimental model. Grx1 or Trx1 silencing caused conspicuous changes in proteome reflected significant reduced/oxidized ratios specific Cys's including several glycolytic enzymes. Cys91 peroxiredoxin-6 (PRDX6) Cys153...
Peyronellaea pinodes causes Ascochyta blight, one of the major diseases in pea worldwide. Cultivated plants have a low resistance to this disease. Although quantitative trait loci (QTLs) involved blight been identified, specific genes associated with these QTLs remain unknown, which makes marker-assisted selection difficult. Complex traits alter proteins and their abundance. Quantitative estimation might therefore be useful selecting potential markers for breeding. In work, we developed...
Alternative splicing is a widespread mechanism of gene expression regulation. Previous analyses based on conventional RT-PCR reported the presence an unspliced c-fos transcript in several mammalian systems. Compared to well-defined knowledge alternative fosB, physiological relevance regulating remains largely unknown. This work aimed investigate functional significance pre-mRNA. A set primers was designed demonstrate that, whereas introns 1 and 2 are regularly spliced from primary...
Proteomics is a crucial tool for unravelling the molecular dynamics of essential biological processes, becoming pivotal technique basic and applied research. Diverse bioinformatic tools are required to manage explore huge amount information obtained from single proteomics experiment. Thus, functional annotation protein–protein interactions evaluated in depth leading conclusions that best fit proteomic response system under study. To gain insight into potential applications identified...
Abstract Protein phosphorylation and membrane proteins play an important role in the infection of plants by phytopathogenic fungi, given their involvement signal transduction cascades. Botrytis cinerea is a well-studied necrotrophic fungus taken as model organism fungal plant pathology, its broad host range adverse economic impact. To elucidate relevant events during infection, several proteomics analyses have been performed B . , but they cover only 10% total predicted genome database this...
Depression and anxiety are common during pregnancy. is associated with preterm birth, low birth weight, intrauterine growth restriction. Risk factors related to depression pregnancy poor social support, quality of intimate relationship, poverty previous episodes anxiety. When these mental disorders untreated, health behaviors observed in pregnant patients including inadequate nutrition, smoking, drug use failure attend medical appointments. Inadequate maternal-fetal attachment developed when...
Microorganisms play unique, essential and integral roles in the biosphere. This work aims to assess utility of soil's metaomics for environmental diagnosis. Doñana National Park (DNP) was selected as a natural lab since it contains strictly protected core that is surrounded by numerous threats pollution. Culture-independent high-throughput molecular tools were used evaluate alterations global structure metabolic activities microbiome. 16S rRNA sequencing shows lower bacterial abundance...
Long-term starvation provokes a metabolic response in the brain to adapt lack of nutrient intake and maintain physiology this organ. Here, we study changes global proteomic profile rat after seven-day period food deprivation, further our understanding biochemical cellular mechanisms underlying situations without food. We have used two-dimensional electrophoresis followed by mass spectrometry (2D-MS) order identify proteins differentially expressed during prolonged deprivation. After...
Perinatal depression is an emerging field with questions that remain to be answered, including the underlying pathogenesis, treatment and counseling. Pregnant women represent a serious risk themselves due negative fetal/obstetrical neonatal outcomes their child respect development later in life. The immune system plays crucial role major disorder (MDD), studies indicate both mediators (cytokines, chemokines) neuroendocrine hormones cross-talk MDD. However, innate unexplored field, its link...
Acetic acid bacteria (AAB) and other members of the complex microbiotas, whose activity is essential for vinegar production, display biodiversity richness that difficult to study in depth due their highly selective culture conditions. In recent years, liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS) has emerged as a powerful tool rapidly identifying thousands proteins present microbial communities, offering broader precision coverage. this work, novel method based on...