A5001966329- Redox biology and oxidative stress
- Heat shock proteins research
- Mitochondrial Function and Pathology
- Nitric Oxide and Endothelin Effects
- Sulfur Compounds in Biology
- Glutathione Transferases and Polymorphisms
- Cancer, Hypoxia, and Metabolism
- Electron Spin Resonance Studies
- Alzheimer's disease research and treatments
- Parkinson's Disease Mechanisms and Treatments
- Fungal and yeast genetics research
- Cholinesterase and Neurodegenerative Diseases
- Metal-Catalyzed Oxygenation Mechanisms
- Marine and coastal ecosystems
- Microbial Community Ecology and Physiology
- Endoplasmic Reticulum Stress and Disease
- ATP Synthase and ATPases Research
- Photosynthetic Processes and Mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Genomics, phytochemicals, and oxidative stress
University of Córdoba
2015-2023
Instituto Maimónides de Investigación Biomédica de Córdoba
2015-2018
Peroxiredoxin 6 (PRDX6) has been associated with tumor progression and cancer metastasis. Its acting on phospholipid hydroperoxides its phospholipase-A2 activity are unique among the peroxiredoxin family add complexity to action mechanisms. As a first step towards study of PRDX6 involvement in cancer, we have constructed human hepatocarcinoma HepG2PRDX6-/- cell line using CRISPR/Cas9 technique characterized cellular response lack PRDX6. Applying quantitative global redox proteomics, flow...
The aim of the present study was to define role Trx and Grx on metabolic thiol redox regulation identify their protein metabolite targets. hepatocarcinoma-derived HepG2 cell line under both normal oxidative/nitrosative conditions by overexpression NO synthase (NOS3) used as experimental model. Grx1 or Trx1 silencing caused conspicuous changes in proteome reflected significant reduced/oxidized ratios specific Cys's including several glycolytic enzymes. Cys91 peroxiredoxin-6 (PRDX6) Cys153...
Nitric oxide (NO) plays relevant roles in signal transduction physiopathology and its effects are dependent on several environmental factors. NO has both pro-apoptotic anti-apoptotic functions but the molecular mechanisms responsible for these opposite not fully understood. The action of occurs mainly through redox changes target proteins, particularly by S-nitrosylation reactive cysteine residues. Thioredoxin (Trx) glutaredoxin (Grx) systems main cellular controllers thiolic state proteins...
Peroxiredoxin 6 (Prdx6) is the only member of 1-Cys subfamily peroxiredoxins in human cells. It Prdx acting on phospholipid hydroperoxides possessing two additional sites with phospholipase A2 (PLA2) and lysophosphatidylcholine-acyl transferase (LPCAT) activities. There are contrasting reports roles mechanisms multifunctional Prdx6 several pathologies its sensitivity to, influence on, redox environment. We have down-regulated specific siRNA hepatoblastoma HepG2 cells to study role cell...
Prochlorococcus requires the capability to accommodate environmental changes in order proliferate oligotrophic oceans, particular regarding nitrogen availability. A precise knowledge of composition and proteome can yield fundamental insights into such a response. Here we report detailed analysis important model cyanobacterium marinus SS120 after treatment with azaserine, an inhibitor ferredoxin-dependent glutamate synthase (GOGAT), simulate extreme starvation. In total, 1,072 proteins,...
DJ-1 is a multifunctional protein involved in Parkinson disease (PD) that can act as antioxidant, molecular chaperone, protease, glyoxalase, and transcriptional regulator. However, the exact mechanism by which dysfunction contributes to development of Parkinson's remains elusive. Here, using comparative proteomic analysis between wild-type cortical neurons lacking (data available via ProteomeXchange, identifier PXD029351), we show this cell cycle checkpoints disruption. We detect increased...
Sorafenib is the first-line recommended therapy for patients with advanced hepatocarcinoma (HCC) in de-differentiation stage (presenting epithelial-mesenchymal transition, EMT). We studied role of thioredoxin system (Trx1/TrxR1) sensitivity or resistance HCC cells to treatment Sorafenib. As a model, we used set three established cell lines different degrees as occurs metastasis. By quantitative proteomics, found that expression levels Trx1 and TrxR1 followed same trend canonical EMT markers...
Peroxiredoxin 6 (PRDX6), the only mammalian 1-Cys member of peroxiredoxin family, has peroxidase, phospholipase A2 (PLA2), and lysophosphatidylcholine (LPC) acyltransferase (LPCAT) activities. It been associated with tumor progression cancer metastasis, but mechanisms involved are not clear. We constructed an SNU475 hepatocarcinoma cell line knockout for PRDX6 to study processes migration invasiveness in these mesenchymal cells. They showed lipid peroxidation inhibition NRF2 transcriptional...
NO has an antiproliferative action on HepG2 cells and Thioredoxin (Trx) Glutaredoxin (Grx) have denitrosilase deglutathionylase activities. To ascertain whether Trx and/or Grx systems intermediate the anti-proliferative effect of hepatoblastoma by modulating redox-state key proteins. overexpressing Nitric Oxide Synthase-3 (NOS-3) were transfected with specific siRNA to silence Trx1 Grx1. The expression thiolic redox state proteins determined Western blot mobility shift assay. Overexpression...