We examined the <i>in vivo</i> expression of up to 16 genes encoding for components both glutaredoxin and thioredoxin systems members OxyR SoxRS regulons. demonstrated that <i>grxA</i> (Grx1) transcription is triggered in bacteria lacking Trx1 (<i>trxA</i>) GSH (<i>gshA</i>) an OxyR-dependent manner. also indicated that, unlike OxyR, SoxR not constitutively activated oxidizing environment <i>trxA gshA</i> mutants. discovered lack plus increases steady-state levels Trx reductase (<i>trxB</i>)...

Escherichia coli has two aerobic ribonucleotide reductases encoded by the nrdAB andnrdHIEF operons. While NrdAB is active during aerobiosis, NrdEF considered a cryptic enzyme with no obvious function. Here, we present evidence that nrdHIEF expression might be important under certain circumstances. Basal transcript levels were dramatically enhanced (25–75-fold), depending on growth-phase and growth-medium composition. Likewise, large increase of >100-fold in mRNA was observed bacteria lacking...

This work provides the first absolute expression patterns of genes coding for all known components both thioredoxin (Trx) and glutaredoxin (Grx) systems in mouse: Trx1, Trx2, Grx1, Grx2, TrxR1, TrxR2, thioredoxin/glutathione reductase, glutathione reductase. We devised a novel assay that, combining advantages multiplex real-time PCR, streamlines quantitation actual mRNA copy numbers whole-animal experiments. Quantitations reported establish differences among adult organs embryonic stages,...

Selenium (Se) is a trace element crucial for human health. Recently, the impact of Se supplementation on gut microbiota has been pointed out as well its influence expression certain selenoproteins and metabolites. This study aims to elucidate link between supplementation, brain metabolome possible connection with gut-brain axis. To this end, an in vivo 40 BALB/c mice was carried out. The included conventional (n=20) model depleted by antibiotics under regular or supplemented diet. Brain...

Abstract We evaluated the suitability of high‐throughput proteomic methods to monitor terrestrial ecosystems. Free‐living Mus spretus from three sites along “Domingo Rubio” (DR) stream were compared with mice Doñana Biological Reserve (“Santa Olalla” lagoon (SOL) negative control), using specimens an industrial settlement (phosphogypsum stacks (PS)) and rice fields (“Matochal” (ARZ)) as positive controls. Our 2‐DE analysis showed 36 spots significantly altered expression. Sixteen identified...

Alternative splicing is a widespread mechanism of gene expression regulation. Previous analyses based on conventional RT-PCR reported the presence an unspliced c-fos transcript in several mammalian systems. Compared to well-defined knowledge alternative fosB, physiological relevance regulating remains largely unknown. This work aimed investigate functional significance pre-mRNA. A set primers was designed demonstrate that, whereas introns 1 and 2 are regularly spliced from primary...

Abasic sites (AP sites) are generated in DNA either directly by DNA-damaging agents or glycosylases acting during base excision repair. These repaired human cells the HAP1 protein, which, besides its AP-endonuclease activity, also possesses a redox function. To investigate ability of protein to modulate cell resistance agents, CHO were transfected with cDNA, resulting stable expression nuclei. The sensitivity toxic effect various e.g. methylmethane sulfonate, bleomycin and H2O2, was not...

Hereditary tyrosinemia type I (HT1) is an autosomal recessive inborn error of metabolism caused by the deficiency fumarylacetoacetate hydrolase, last enzyme in tyrosine catabolism pathway. This defect results accumulation succinylacetone (SA) that reacts with amino acids and proteins to form stable adducts via Schiff base formation, lysine being most reactive acid. HT1 patients surviving beyond infancy are at considerable risk for development hepatocellular carcinoma, a high level...

Most studies using real-time PCR are taken semiquantitatively and assume a steady level of expression forthe so-called housekeeping genes. By absolute we demonstrate that the transcript amounts two most popular internall controls (coding GAPDH beta-actin) fluctuate dramatically across diverse mouse or human tissues. This raises question about inaccuracy these genes squantitative references in tissue-specific mRNA profiling. Target chosen for analysis involved DNA repair, regulation gene...

Abstract In this work we report on the isolation of an Escherichia coli K‐12 mutation, which confers a high sensitivity to bacteria cells mutagenesis by simple monofunctional alkylating agents. The mutation emerged spontaneously from bacterial strain that already proved useful in various mutagenicity studies. By monitoring influence such frequency induced ethylating (EMS, DES, ENU, ENNG) vs. methylating (MMS, DMS, MNU, MNNG) compounds, and vivo repair capacity for different alkyl‐DNA lesions...

Journal Article Mutagenesis in Escherichia coli K-12 mutants defective superoxide dismutase or catalase Get access María-José Prieto-Alamo, Prieto-Alamo Departmento de Genética, Facultad Ciencias, Universidad Córdoba14071-Córdoba, Espana Search for other works by this author on: Oxford Academic PubMed Google Scholar Nieves Abril, Abril Carmen Pueyo 1 1To whom reprint requests should be sent Carcinogenesis, Volume 14, Issue 2, February 1993, Pages 237–244,...

Abstract Here we report a preliminary working scheme for the integrative application of transcriptomic, proteomic, and metallomic methodologies in environmental monitoring, by using as sentinel wildlife species Mus spretus reference gene/protein sequence databases from key model musculus . We have demonstrated that absolute transcript expression signatures quantified reverse transcription (RT) real-time polymerase chain reaction (PCR) selected genes (e.g., those coding biotransformation...

Abstract We examined the role of O 6 ‐alkylguanine‐DNA alkyltransferase encoded by ogt gene in sensitivity Escherichia coli to mutagenic effects dibromoalkanes, dibromoethane and dibromomethane, comparing responses − bacteria those their isogenic + parental counterparts. The uvrABC excision‐repair system, adaptive response, mucAB umuDC processing, glutathione bioactivation on differential were also studied. Mutation induction was monitored measuring frequency forward mutations L ‐arabinose...

Reactive oxygen species produce different lesions in DNA. Among them, 7,8-dihydro-8-oxoguanine (8-oxoG) is one of the major oxidative products implicated mutagenesis. This lesion removed from damaged DNA by base excision repair, and genes coding for 8-oxoG-DNA glycosylases have been isolated bacteria, yeast human cells. We characterized cDNA encoding rat glycosylase (rOGG1). Expression fgp mutY Escherichia coli double mutant allowed purification untagged rOGG1 protein. It excises 8-oxoG with...

This article reports the first absolute quantitative analysis of expression patterns murine transcripts (Gsta1/2, Gsta3, Gsta4, Gstm1, Gstm2, Gstm3, Gsto1, Gstp1/2, Gstt1, Gstt2) coding for most glutathione S-transferases (GSTs) alpha, mu, omega, pi, and theta classes. We examine how steady-state numbers are modulated in association with: three animal organs (liver, kidney, lung) where extensive detoxification occurs; two species (Mus musculus Mus spretus) representing common laboratory...