A5089031039- Cassava research and cyanide
- Wastewater Treatment and Nitrogen Removal
- Microbial Fuel Cells and Bioremediation
- Amino Acid Enzymes and Metabolism
- Microbial Community Ecology and Physiology
- Plant nutrient uptake and metabolism
- Chromium effects and bioremediation
- Legume Nitrogen Fixing Symbiosis
- Genomics and Phylogenetic Studies
- Polyamine Metabolism and Applications
- Photosynthetic Processes and Mechanisms
- Metalloenzymes and iron-sulfur proteins
- Microbial bioremediation and biosurfactants
- Enzyme Structure and Function
- Arsenic contamination and mitigation
- Folate and B Vitamins Research
- Chemical Analysis and Environmental Impact
- Microbial Applications in Construction Materials
- Advanced oxidation water treatment
- Enzyme Production and Characterization
- Ammonia Synthesis and Nitrogen Reduction
- Algal biology and biofuel production
- Porphyrin Metabolism and Disorders
- Marine and coastal ecosystems
- Metabolism and Genetic Disorders
University of Córdoba
2015-2024
Bielefeld University
1989
ABSTRACT A bacterial strain able to use cyanide as the sole nitrogen source under alkaline conditions has been isolated. The bacterium was classified Pseudomonas pseudoalcaligenes by comparison of its 16S RNA gene sequence those existing strains and deposited in Colección Española de Cultivos Tipo (Spanish Type Culture Collection) CECT5344. Cyanide consumption is an assimilative process, since (i) growth concomitant proportional degradation (ii) stoichiometrically converted into ammonium...
Denitrification is a respiratory process that produces nitrous oxide as an intermediate, which may escape to the atmosphere before its reduction dinitrogen through reductase NosZ. In this work, denitrification carried out by Paracoccus denitrificans PD1222 has been explored quantitative proteomic analysis. Under anaerobic conditions, with nitrate sole nitrogen source, synthesis of all enzymes involved in denitrification, nitrate, nitrite, nitric and reductases, was increased. However,...
Summary The prototrophic bacterium Rhodobacter sphaeroides DSM 158 has a periplasmic nitrate reductase which is induced by and it not repressed ammonium or oxygen. In Tn 5 mutant lacking activity, transposon insertion localized in 1.2 kb Eco RI fragment. A 0.6 Bam HI‐ segment of this region was used as probe to isolate, from the wild‐type strain, 6.8 Pst l fragment carrying putative genes coding for reductase. vivo protein expression DNA sequence analysis reveal presence three genes, napABC...
DNA sequence analysis of a 1,600-base-pair fragment located downstream nifENX in nif region A Rhodobacter capsulatus revealed two additional open reading frames (ORFs): ORF5, encoding ferredoxinlike protein, and nifQ. The gene product contained cysteine motifs, typical ferredoxins coordinating 4Fe-4S clusters, but the distance between these motifs was unusual for low-molecular-weight ferredoxins. R. nifQ shared high degree homology with Klebsiella pneumoniae Azotobacter vinelandii NifQ,...
Abstract Denitrification is a respiratory process by which nitrate reduced to dinitrogen. Incomplete denitrification results in the emission of greenhouse gas nitrous oxide and this potentiated acidic soils, display rates high N 2 O/N ratios compared alkaline soils. In work, impact pH on proteome soil denitrifying bacterium Paracoccus denitrificans PD1222 was analysed with as sole energy nitrogen source under anaerobic conditions at ranging from 6.5 7.5. Quantitative proteomic analysis...
Rhodobacter capsulatus E1F1 grows phototrophically with nitrate as nitrogen source. Using primers designed for conserved motifs in bacterial assimilatory reductases, a 450-bp DNA was amplified by PCR and used the screening of genomic library. A cosmid carrying an insert four SalI fragments 2.8, 4.1, 4.5, 5.8 kb isolated, sequencing revealed that it contains assimilation (nas) gene region, including hcp coding hybrid cluster protein (HCP). Expression is probably regulated nitrite-sensitive...
ABSTRACT Cyanase catalyzes the decomposition of cyanate into CO 2 and ammonium, with carbamate as an unstable intermediate. The cyanase Pseudomonas pseudoalcaligenes CECT5344 was negatively regulated by ammonium positively cyanate, cyanide, some cyanometallic complexes. activity not detected in cell extracts from cells grown even presence cyanate. Nevertheless, a low level nitrogen-starved cells. cyn gene cluster P. cloned analyzed. cynA, cynB , cynD genes encode ABC-type transporter, cynS...
Mining, jewellery and metal-processing industries use cyanide for extracting gold other valuable metals, generating large amounts of highly toxic wastewater. Biological treatments may be a clean alternative under the environmental point view to conventional physical or chemical processes used remove related compounds from these industrial effluents. Pseudomonas pseudoalcaligenes CECT5344 can grow alkaline conditions using cyanide, cyanate different nitriles as sole nitrogen source, is able...
Science & Society20 October 2021Open Access Bioremediation of cyanide-containing wastes The potential systems and synthetic biology for cleaning up the toxic leftovers from mining María Dolores Roldán Corresponding Author [email protected] Departamento de Bioquímica y Biología Molecular, Edificio Severo Ochoa, Universidad Córdoba, Spain Search more papers by this author Alfonso Olaya-Abril Lara P Sáez Purificación Cabello Botánica, Ecología Fisiología Vegetal, Celestino Mutis, Víctor M...
Pseudomonas pseudoalcaligenes CECT5344 is a Gram-negative bacterium able to tolerate cyanide and use it as the sole nitrogen source. We report here first draft of whole genome sequence P. strain that assimilates cyanide. Three aspects are specially emphasized in this manuscript. First, some generalities shown discussed context other Pseudomonadaceae genomes, including size, G + C content, core singletons among features. Second, analysed metabolism, describing genes probably involved...
The phototrophic bacterium Rhodobacter sphaeroides DSM 158 is able to reduce nitrate nitrite by means of a periplasmic reductase which induced and not repressed ammonium or oxygen. Recently, 6.8 kb PstI DNA fragment carrying the napABC genes coding for this nitrate-reducing system was cloned [Reyes, Roldán, Klipp, Castillo Moreno-Vivián (1996) Mol. Microbiol. 19, 1307-1318]. Further sequence genetic analyses region upstream from reveal presence four additional nap genes. All these R. seem be...
ABSTRACT Bacterial periplasmic nitrate reductases (Nap) can play different physiological roles and are expressed under conditions depending on the organism. Rhodobacter sphaeroides DSM158 has a Nap system, encoded by napKEFDABC gene cluster, but nitrite formed is not further reduced because this strain lacks reductase. activity increases in presence of oxygen unaffected ammonium. Reverse transcription-PCR Northern blots demonstrated that genes constitute an operon transcribed as single...