A5078520878- Plant-Microbe Interactions and Immunity
- Antimicrobial Peptides and Activities
- Biochemical and Structural Characterization
- Plant Virus Research Studies
- Plant Pathogens and Fungal Diseases
- Transgenic Plants and Applications
- Protein Hydrolysis and Bioactive Peptides
- Plant tissue culture and regeneration
- Plant and Fungal Interactions Research
- Environmental and sustainability education
- Microbial Natural Products and Biosynthesis
- Fungal Biology and Applications
- Postharvest Quality and Shelf Life Management
- Plant Reproductive Biology
- Studies on Chitinases and Chitosanases
- Mycotoxins in Agriculture and Food
- Insect and Pesticide Research
- Entomopathogenic Microorganisms in Pest Control
- Fungal and yeast genetics research
- CRISPR and Genetic Engineering
- Plant Physiology and Cultivation Studies
- Plant Pathogenic Bacteria Studies
- Antifungal resistance and susceptibility
- Polydiacetylene-based materials and applications
- Invertebrate Immune Response Mechanisms
Universitat de València
2021-2025
Instituto de Agroquímica y Tecnología de Alimentos
2014-2024
Consejo Superior de Investigaciones Científicas
2014-2024
University of Edinburgh
2012
Instituto Valenciano de Investigaciones Agrarias
2005-2007
Antimicrobial peptides (AMP) are widely recognized as promising alternatives to the current use of antibiotics and fungicides. Amino-acid sequences a vast majority AMP share cationic amphipathic biophysical properties that allow their insertion into lipid bilayers can lead alteration biological membrane functions. Initial characterization studies linked these antimicrobial killing activity. However, further data indicate this is not sole mode action more subtle mechanisms might mediate...
Antifungal proteins of fungal origin (AFPs) are small, secreted, cationic, and cysteine-rich proteins. Filamentous fungi encode a wide repertoire AFPs belonging to different phylogenetic classes, which offer great potential develop new antifungals for the control pathogenic fungi. The fungus Penicillium expansum is one few reported three each class (A, B, C). In this work, production putative from P. was evaluated, but only representative A, PeAfpA, identified in culture supernatants native...
Filamentous fungi encode distinct antifungal proteins (AFPs) that offer great potential to develop new antifungals. Fungi are considered immune their own AFPs as occurs in Penicillium chrysogenum, the producer of well-known PAF. The digitatum genome encodes only one afp gene (afpB), and corresponding protein (AfpB) belongs class B phylogenetic cluster. Previous attempts detect AfpB were not successful. In this work, immunodetection confirmed absence accumulation wild type previous...
The activity of protein O-mannosyltransferases (Pmts) affects the morphogenesis and virulence fungal pathogens. Recently, PMT genes have been shown to determine sensitivity Saccharomyces cerevisiae antifungal peptide PAF26. This study reports identification characterization three Pdpmt in citrus post-harvest pathogen Penicillium digitatum. are expressed during growth fruit infection, with highest induction for Pdpmt2. Pdpmt2 complemented defect S. Δpmt2 strain. gene mutation P. digitatum...
Penicillium phytopathogenic species provoke severe postharvest disease and economic losses. expansum is the main pome fruit phytopathogen while digitatum italicum cause citrus green blue mold, respectively. Control strategies rely on use of synthetic fungicides, but appearance resistant strains safety concerns have led to search for new antifungals. Here, potential application different antifungal proteins (AFPs) including three chrysogenum (PAF, PAFB PAFC), as well Neosartorya fischeri...
Fungal infections threaten human health worldwide and have a negative impact on food security, damaging crop production causing animal diseases. At present, only few classes of fungicides are available due to the complexity targeting fungi without affecting plant, animal, or hosts.
Fungal synthetic biology is a rapidly expanding field that aims to optimize the biotechnological exploitation of fungi through generation standard, ready-to-use genetic elements, and universal syntax rules for contributory use by fungal research community. Recently, an increasing number toolkits have been developed applied filamentous fungi, which highlights relevance these organisms in biotechnology field. The FungalBraid (FB) modular cloning platform enables interchangeability DNA parts...
Fermented plant-based beverages are renowned due to their health benefits and sustainability. This study focuses on developing fermented local from rice, carob, tiger nuts. The fermentation process with four different commercial starters of lactic acid bacteria was optimized based pH drop colony counts at 37 °C the supplementation 7.5–15 g glucose/100 mL. Analyses antioxidant capacity, phytochemical profile, proximate composition sensory attributes were conducted, along studies...
The mechanism of action antimicrobial peptides (AMP) was initially correlated with peptide membrane permeation properties. However, recent evidences indicate that a number AMP is more complex and involves specific interactions at cell envelopes or intracellular targets. In this study, genomic approach undertaken on the model yeast Saccharomyces cerevisiae to characterize antifungal effect two unrelated AMP.Two differentiated were used: synthetic cell-penetrating PAF26 natural cytolytic...
Antifungal proteins (AFPs) from Ascomycetes are small cysteine-rich that abundantly secreted and show antifungal activity against non-producer fungi. A gene coding for a class B AFP (AfpB) was previously identified in the genome of plant pathogen Penicillium digitatum. However, previous attempts to detect AfpB protein were not successful despite high expression corresponding afpB gene. In this work, structure putative modeled. Based on model, four synthetic cysteine-containing peptides,...
Filamentous fungi produce small cysteine-rich proteins with potent, specific antifungal activity, offering the potential to fight fungal infections that severely threaten human health and food safety security. The genome of citrus postharvest pathogen Penicillium digitatum encodes one these proteins, namely AfpB. Biotechnologically produced AfpB inhibited growth major pathogenic at minimal concentrations, surprisingly including its parental fungus, conferred protection crop plants against...
Summary Fungal antifungal proteins (AFPs) have attracted attention as novel biofungicides. Their exploitation requires safe and cost‐effective producing biofactories. Previously, Penicillium chrysogenum digitatum produced recombinant AFPs with the use of a P. ‐based expression system that consisted paf gene promoter, signal peptide (SP)‐pro sequence terminator. Here, regulatory elements afpA encoding highly PeAfpA from expansum were developed an for AFP production through FungalBraid...