Metabolites reflect the integration of gene expression, protein interaction and other different regulatory processes are therefore closer to phenotype than mRNA transcripts or proteins alone. Amongst all –omics technologies, metabolomics is most transversal can be applied organisms with little no modifications. It has been successfully study molecular phenotypes plants in response abiotic stress order find particular patterns associated tolerance. These studies have highlighted essential...

Soil flooding constitutes a seasonal factor that negatively affects plant performance and crop yields. In this work, the relationship between oxidative damage sensitivity was addressed in three citrus genotypes with different abilities to tolerate waterlogging. We examined leaf visible damage, terms of malondialdehyde (MDA) concentration, proline root ascorbate glutathione contents antioxidant enzyme activities superoxide dismutase (EC 1.15.1.1), peroxidase 1.11.1.11), catalase 1.11.1.6)...

In the field, plants experience high light (HL) intensities that are often accompanied by elevated temperatures. Such conditions a serious threat to agriculture production, because photosynthesis is highly sensitive both HL and high-temperature stress. One of potential cellular targets heat stress (HS) combination PSII its degree photoinhibition depends on balance between rate damage (induced stress), repair (impaired under HS). Here, we studied responses Arabidopsis (Arabidopsis thaliana)...

Phytohormones are central players in sensing and signaling numerous environmental conditions like drought stress. In this work, an experimental system based on severe was established hormone profiling together with gene expression of key enzymes involved abscisic acid (ABA) jasmonic (JA) biosynthesis studied roots citrumelo CPB 4475 (a commercial citrus rootstock) plants. JA concentration transiently increased after a few hours stress, returning to control levels 30 h the onset condition. A...

In natural environments, several adverse environmental conditions occur simultaneously constituting a unique stress factor. this work, physiological parameters and the hormonal regulation of Carrizo citrange Cleopatra mandarin, two citrus genotypes, in response to combined action high temperatures water deprivation were studied. The objective was characterize particular responses combination. Experiments indicated that is more tolerant combination than mandarin. Furthermore, an experimental...

Drought and high temperatures are two major abiotic stress factors that often occur simultaneously in nature, affecting negatively crop performance yield. Moreover, these environmental challenges induce oxidative plants through the production of reactive oxygen species (ROS). Carrizo citrange Cleopatra mandarin citrus genotypes with contrasting ability to cope combination drought heat stress. In this work, a direct relationship between an increased antioxidant activity tolerance is reported....

Drought and heat stresses are two of the most frequent environmental factors that take place simultaneously in field constraining global crop productivity. Metabolism reconfiguration is often behind adaptation plants to adverse conditions. Carrizo citrange Cleopatra mandarin, citrus genotypes with contrasting ability tolerate combined drought conditions, showed different metabolite patterns. Increased levels phenylpropanoid metabolites were observed response stress, including scopolin, a...

Abstract Abscisic acid (ABA) plays a key role in plant acclimation to abiotic stress. Although recent studies suggested that ABA could also be important for combination of stresses, its this response is currently unknown. Here we studied the mutants impaired signalling ( abi1-1 ) and biosynthesis aba1-1 water deficit heat Both displayed reduced growth, biomass, survival when subjected stress combination. Focusing on , found although stomata had an deficit, remaining significantly more open...

Light stress in one part of a plant is communicated to other leaves coordinate stomatal responses.

A rapid multiresidue method to quantify three different classes of plant hormones has been developed. The reduced concentrations these metabolites in real samples with complex matrixes require sensitive techniques for their quantification small amounts tissue. described combines high-performance liquid chromatography electrospray ionization tandem mass spectrometry. Deuterium-labeled standards were added prior sample extraction achieve an accurate abscisic acid, indole-3-acetic and jasmonic...

The antagonism between gibberellins (GA) and abscisic acid (ABA) is an important factor regulating the developmental transition from embryogenesis to seed germination. In barley aleurone layers, expression of genes encoding α-amylases proteases induced by GA but suppressed ABA. It has been shown that ABA-induced protein kinase, PKABA1, mediates ABA suppression α-amylase expression. Using a transient system, we have now localized site action PKABA1 relative other signal transduction...

The phytohormone abscisic acid (ABA) induces genes-encoding proteins involved in desiccation tolerance and dormancy seeds, but ABA also suppresses gibberellin (GA)-responsive genes encoding hydrolytic enzymes essential for postgermination growth. A unique serine/threonine protein kinase, PKABA1 mRNA, up-regulated by has been identified. In this report, the effect of on signal transduction pathway mediating induction suppression determined aleurone layers barley seeds. Two groups gene...

In this work, seedlings of two citrus rootstocks, the salt-tolerant Cleopatra mandarin (Citrus reshni Hort. ex Tan.) and salt-sensitive Carrizo citrange sinensis [L.] Osb. x Poncirus trifoliata Raf.) were used to study relationship between chloride water uptake. The results indicated that net uptake rates in both genotypes alike decreased linearly with time salinity exposure, although they more rapidly reduced tolerant genotype. each rootstock, paralleled decreases transpiration rates. When...

The involvement of abscisic acid (ABA) in the process leaf abscission induced by 1-aminocyclopropane-1-carboxylic (ACC) transported from roots to shoots Cleopatra mandarin (Citrus reshni Hort. ex Tan.) seedlings grown under water stress was studied using norflurazon (NF). Water both ABA (24-fold) and ACC (16-fold) accumulation arrested xylem flow. Leaf bulk also increased (8-fold), although did not occur. Shortly after rehydration, root returned their prestress levels, whereas sharp...

Seed dormancy prevents seeds from germinating under environmental conditions unfavourable for plant growth and development constitutes an evolutionary advantage. Dry storage, also known as after-ripening, gradually decreases seed by mechanisms not well understood. An Arabidopsis thaliana DOF transcription factor gene (DOF6) affecting germination has been characterized. The transcript levels of this accumulate in dry decay during after-ripening upon imbibition. While constitutive...