Significance N 6 -methyladenosine (m A) modification has been found to constitute an important regulatory mechanism in RNA biology. Unlike mammals and yeast, no component of the m A cellular machinery described plants at present. Although influence m6A suspected occur plant virus cycle, it never proved. Here we have identified a protein with demethylase activity (atALKBH9B) demonstrate that this removes from vitro. Remarkably, abundance on viral genome alfalfa mosaic is influenced by...

Fruit-set and growth in tomato depend on the action of gibberellins (GAs). To evaluate role GA biosynthetic enzyme 20-oxidase (GA20ox) that process, citrus gene CcGA20ox1 was overexpressed (Solanum lycopersicum L.) cv Micro-Tom. The transformed plants were taller, had non-serrated leaves, some flowers displayed a protruding stigma due to longer style, thus preventing self-pollination, similar GA3-treated plants. Flowering delayed compared with wild-type (WT) Both yield number fruits per...

We have performed an extensive study on the responses to salt stress in four related Limonium halophytes with different geographic distribution patterns, during seed germination and early vegetative growth. The aims of work were twofold: establish basis for chorology these species, identify relevant mechanisms tolerance dependent control ion transport osmolyte accumulation. Seeds germinated vitro, presence increasing NaCl concentrations, subjected 'recovery germination' tests; percentages...

Abstract Introduction – Plants utilise vaious defence mechanisms against their potential biotic stressing agents such as viroids, viruses, bacteria or fungi and abiotic environmental challenges. Among them metabolic alteration is a common response in both compatible incompatible plant–pathogen interactions. However, the identification of changes associated with not an easy task due to complexity metabolome plant response. To address problem complexity, metabolomics approach was employed this...

Eggplant (Solanum melongena) varieties with increased levels of phenolics in the fruit present enhanced functional quality, but may display greater flesh browning. We evaluated 18 eggplant accessions for total content, chlorogenic acid DPPH scavenging activity, polyphenol oxidase (PPO) liquid extract browning, and For all traits we found a high diversity, differences among up to 3.36-fold Variation content accounted only 18.9% 6.0% variation PPO activity was not significantly correlated...

Hydroxycinnamic acid amides (HCAA) are secondary metabolites involved in plant development and defense that have been widely reported throughout the kingdom. These phenolics show antioxidant, antiviral, antibacterial, antifungal activities. Hydroxycinnamoyl-CoA:tyramine N-hydroxycinnamoyl transferase (THT) is key enzyme HCAA synthesis induced response to pathogen infection, wounding, or elicitor treatments, preceding accumulation. We engineered transgenic tomato plants overexpressing THT....

Comparative studies on the responses to salt stress of taxonomically related taxa should help elucidate relevant mechanisms tolerance in plants. We have applied this strategy three Plantago species adapted different natural habitats, P. crassifolia and coronopus–both halophytes–and major, considered as salt-sensitive since it is never found saline habitats. Growth inhibition measurements controlled treatments indicated, however, that major quite resistant stress, although less than its...

Tomato plants expressing the NahG transgene, which prevents accumulation of endogenous salicylic acid (SA), were used to study importance SA signalling pathway in basal defence against Citrus Exocortis Viroid (CEVd) or Spotted Wilt Virus (TSWV). The lack CEVd- TSWV-infected tomato led an early and dramatic disease phenotype, as compared that observed corresponding parental Money Maker. Addition acibenzolar-S-methyl, a benzothiadiazole (BTH), activates systemic acquired resistance downstream...

Forest species ranges are confined by environmental limitations such as cold stress. The natural range shifts of pine forests due to climate change and proactive-assisted population migration may each be constrained the ability tolerate low temperatures, especially in northern latitudes or high altitudes. aim this study is characterize response cold-tolerant versus cold-sensitive Pinus halepensis (P. halepensis) seedlings at physiological molecular level under controlled conditions identify...

Inoculation of tomato plants (Solanum lycopersicum cv. Rutgers) with Pseudomonas syringae pv. led to the production a hypersensitive-like response in this pathovar tomato. Accumulation hydroxycinnamic acid amides (HCAA) tyramine (p-coumaroyltyramine and feruloyltyramine) dopamine (p-coumaroyldopamine feruloyldopamine) was detected after bacterial infection. Two them, p-coumaroyldopamine feruloyldopamine, are described for first time. The accumulation HCAA preceded by an increment...

Dittrichia viscosa is a Mediterranean ruderal species that over the last decades has expanded into new habitats, including coastal salt marshes, ecosystems are per se fragile and threatened by human activities. To assess potential risk this native-invasive represents for genuine marsh vegetation, we compared its distribution with of Inula crithmoides, taxonomically related halophyte, in three marshes located 'La Albufera' Natural Park, near city Valencia (East Spain). The presence D. was...

The evaluation of biochemical markers is important for the understanding mechanisms tolerance to salinity Phaseolus beans. We have evaluated several growth parameters in young plants three vulgaris cultivars subjected four levels (0, 50, 100, and 150 mM NaCl); one cultivar P. coccineus, a closely related species reported as more salt tolerant than common bean, was included external reference. Biochemical leaves concentrations ions (Na+, K+, Cl−), osmolytes (proline, glycine betaine, total...

Aroma profile and carotenoids content of melon flesh are two important aspects influencing the quality this fruit that have been characterized using only selected genotypes. However, extant variability whole species remains unknown.A complete view volatile/carotenoid profiles was obtained analyzing 71 accessions, representing diversity species. Gas chromatography-mass spectrometry high-performance liquid chromatography were used to analyze 200 volatile compounds five carotenoids. Genotypes...

The volatile esters of (Z)-3-hexenol with acetic, propionic, isobutyric, or butyric acids are synthesized by alcohol acyltransferases (AAT) in plants. These compounds differentially emitted when tomato plants efficiently resisting an infection Pseudomonas syringae pv. tomato. We have studied the defensive role these green leaf (GLV) response to bacterial infection, analyzing induction resistance mediated GLVs and phenotype upon impaired their biosynthesis. observed that treatments...

Comparative studies on the responses to salinity and drought were carried out in three Juncus species, two halophytes (Juncus maritimus Lam. acutus L.) one more salt-sensitive articulatus L.). Salt tolerance depends inhibition of transport toxic ions aerial part. In taxa studied Na+ Cl- accumulated same extent roots salt treated plants; however, ion contents lower shoots correlated with relative sensitivity lowest levels measured halophytes. Activation K+ at high concentration could also...

Volatile organic compounds (VOCs) emitted by plants are secondary metabolites that mediate the plant interaction with pathogens and herbivores. These may perform direct defensive functions, i.e. acting as antioxidant, antibacterial or antifungal agents, indirectly signalling activation of plant's responses. Using a non-targeted GC-MS metabolomics approach, we identified profile VOCs associated differential immune response Rio Grande tomato leaves infected either virulent avirulent strains...

Transcriptional regulators based on CRISPR architecture expand our ability to reprogramme endogenous gene expression in plants. One of their potential applications is the customization plant metabolome through activation selected enzymes a given metabolic pathway. Using previously described multiplexable activator dCasEV2.1, we assayed selective enrichment Nicotiana benthamiana leaves four different flavonoids, namely, naringenin, eriodictyol, kaempferol, and quercetin. After careful...

Background:Citrus Exocortis Viroid (CEVd) is a non-coding RNA pathogen capable of infecting wide range plant species, despite its lack protein-coding ability. infections induce significant alterations in various physiological and biochemical processes, particularly impacting metabolism. This study shows the metabolic changes upon viroid infection tomato plants (Solanum lycopersicum var. ‘MoneyMaker’) exhibiting altered levels salicylic acid (SA), key signal molecule involved defence against...

Many plastidial isoprenoids, including diterpenes and photosynthesis-related isoprenoids such as carotenoids chlorophylls, derive from C20 geranylgeranyl diphosphate (GGPP), produced by GGPP synthase (GGPPS) enzymes. Heterodimers of GGPPS non-catalytic type I small subunit (SSU-I) proteins produce C10 geranyl (GPP), the precursor monoterpenes. Three isoforms, referred to SlG1-3, are present in tomato (Solanum lycopersicum). Here we explored their contribution production volatile organic...