A5040596947- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Hepatitis C virus research
- Plant Pathogenic Bacteria Studies
- RNA and protein synthesis mechanisms
- Insect-Plant Interactions and Control
- HIV Research and Treatment
- Plant Molecular Biology Research
- Plant Pathogens and Resistance
- Plant-Microbe Interactions and Immunity
- Viral Infections and Immunology Research
- interferon and immune responses
- Hepatitis B Virus Studies
- Chromosomal and Genetic Variations
- Animal Virus Infections Studies
- Protease and Inhibitor Mechanisms
- Plant tissue culture and regeneration
- RNA regulation and disease
- Antimicrobial Peptides and Activities
- Genetic diversity and population structure
- Agricultural Practices and Plant Genetics
- Phytoplasmas and Hemiptera pathogens
- Horticultural and Viticultural Research
- Ubiquitin and proteasome pathways
- MicroRNA in disease regulation
Foundation for Research and Technology Hellas
2015-2025
University of Crete
2016-2025
Clinica de Pneumologie Iaşi
2021
Medizinische Hochschule Hannover
2020
Pasteur Hellenic Institute
2009-2017
Institut Pasteur
2011
Université de Strasbourg
2009
Centre National de la Recherche Scientifique
2009
Institut de Biologie Moléculaire des Plantes
2009
Viroids are self replicating non-coding RNAs capable of infecting a wide range plant hosts. They do not encode any proteins, thus the mechanism by which they escape defenses remains unclear. RNAi silencing is major defense against virus infections, with four DCL proteins being principal components pathway. We have used Nicotiana benthamiana as model to study Potato spindle tuber viroid infection. This member Pospiviroidae family and replicates in nucleus via an asymmetric rolling circle...
Potato (Solanum tuberosum L) is a natural host of spindle tuber viroid (PSTVd) which can cause characteristic symptoms on developing plants including stunting phenotype and distortion leaves tubers. PSTVd the type species family Pospiviroidae, replicate in nucleus move systemically throughout plant. It not well understood how affect genes for successful invasion show altered expression levels upon infection. Our primary focus this study identification formation since infection strongly...
Viroids are small, circular, highly structured pathogens that infect a broad range of plants, causing economic losses. Since their discovery in the 1970s, they have been considered as non-coding pathogens. In last few years, other RNA entities, similar terms size and structure, were shown to be translated (e.g., cirRNAs, precursors miRNA, satellites) well studies showing some viroids located ribosomes, reignited idea may translated. this study, we used advanced bioinformatic analysis, vitro...
RNA silencing is a universal mechanism involved in development, epigenetic modifications and responses to biotic abiotic stresses. The major components of this are Dicer-like (DCL), Argonaute (AGO) RNA-dependent polymerase (RDR) proteins. Understanding the role each component great scientific agronomic importance. Plants, including Nicotiana benthamiana, an important plant model, usually possess four DCL proteins, which has specific role, namely being responsible for production exclusive...
Zucchini yellow mosaic virus (ZYMV) induces serious diseases in cucurbits. To create a tool to screen for resistance genes, we cloned wild ZYMV isolate and inserted the visual marker Rosea1 obtain recombinant clone ZYMV-Ros1. While some plant-virus combinations accumulation of anthocyanins infected tissues, ZYMV-Ros1 infection cucurbits did not lead detectable anthocyanin accumulation. However, induce dark red pigmentation tissues model plant Nicotiana benthamiana. In this species,...
Alpha-1 antitrypsin (AAT) is a major inhibitor of serine proteases in mammals. Therefore, its deficiency leads to protease-antiprotease imbalance and risk for developing lung emphysema. Although therapy with human plasma-purified AAT attenuates deficiency-related emphysema, impact on antibacterial immunity poorly defined. Here, we examined the effect protective AAT-deficient (KO) mice challenged Streptococcus pneumoniae. AAT-KO were highly susceptible S. pneumoniae, as determined by severe...
The prototype foamy virus (PFV) is a nonpathogenic retrovirus that shows promise as vector for gene transfer. PFV (pre)genomic RNA starts with long complex leader can be folded into an elongated hairpin, suggesting alternative strategy to cap-dependent linear scanning translation initiation of the downstream GAG open reading frame (ORF). We found carries several short ORFs (sORFs), three 5′-proximal sORFs located upstream structural element. Scanning-inhibitory hairpin insertion analysis...
In recent years, many viruses that infect grapevine (Vitis vinifera), have been identified in Greece (Katsarou et al., 2023; Panailidou 2023). Further investigation to determine the presence of other known and unknown commercial vineyards has led detection Grapevine pararetrovirus (GPRV). GPRV was discovered 2022 Russia (Republic Dagestan) samples showing symptoms viral disease (Shvets 2022). is a (family Caulimoviridae) whose genome circular double-stranded DNA ranging from 7.2 8.5 Kb size...
Abstract Extracellular vesicles (EVs) are small membranous secreted by cells into their surrounding extracellular environment. Similar to mammalian EVs, plant EVs have emerged as essential mediators of intercellular communication in plants that facilitate the transfer biological material between cells. They also play roles diverse physiological processes including stress responses, developmental regulation, and defense mechanisms against pathogens. In addition, demonstrated promising health...
Viroids are small circular RNAs infecting a wide range of plants. They do not code for any protein or peptide and therefore rely on their structure biological cycle. Observed phenotypes viroid infected plants thought to occur through changes at the transcriptional/translational level host. A mechanism involved in such is RNA-directed DNA methylation (RdDM). Till today, there contradictory works about viroids interference RdDM. In this study, we investigated epigenetic effect infection...
In plants, RNA silencing functions as a potent antiviral mechanism. Virus-derived double-stranded RNAs (dsRNAs) trigger this mechanism, being cleaved by Dicer-like (DCL) enzymes into virus small (vsRNAs). These vsRNAs guide sequence-specific degradation upon their incorporation an RNA-induced complex (RISC) that contains slicer of the Argonaute (AGO) family. Host dependent-RNA polymerases, particularly RDR6, strengthen generating more dsRNA templates from RISC-cleavage products that, in...
Using high-throughput sequencing, we identified a novel carlavirus sequence in 28-year-old 'Kotsifali' grapevine sample collected Heraklion (Crete, Greece). RT-PCR and 5'/3' RACE together with Sanger the complete genome of 8299 nt was confirmed found to contain five open reading frames (ORFs) but lack an ORF6, which is present some members genus Carlavirus. The most similar those two carlaviruses infecting caper, taking into account ICTV nomenclature, propose name "grapevine 1" for this new virus.
HomePlant DiseaseVol. 104, No. 6First Report of Grapevine Yellow Speckle Viroid-2 in Greece PreviousNext DISEASE NOTES OPENOpen Access licenseFirst GreeceC.-L. Sassalou, K. Katsarou, L. Lotos, C. G. Orfanidou, V. I. Maliogka, Kalantidis, N. Katis, and P. PappiC.-L. SassalouLaboratory Plant Pathology, School Agriculture, Faculty Forestry Natural Environment, Aristotle University Thessaloniki, 54124, Greece, KatsarouInstitute Molecular Biology Biotechnology, Foundation for Research...