A5065149304- Systemic Lupus Erythematosus Research
- RNA Interference and Gene Delivery
- Cytokine Signaling Pathways and Interactions
- Genomics and Chromatin Dynamics
- Immunotherapy and Immune Responses
- CRISPR and Genetic Engineering
- Cancer Immunotherapy and Biomarkers
- RNA Research and Splicing
- DNA Repair Mechanisms
- Neurogenesis and neuroplasticity mechanisms
- RNA and protein synthesis mechanisms
- Immune cells in cancer
- DNA and Nucleic Acid Chemistry
- Virus-based gene therapy research
- Extracellular vesicles in disease
- Immune Cell Function and Interaction
- Polymer Nanocomposite Synthesis and Irradiation
- Neutropenia and Cancer Infections
- CAR-T cell therapy research
- Rheumatoid Arthritis Research and Therapies
- Epigenetics and DNA Methylation
- Nerve injury and regeneration
- Hydrogels: synthesis, properties, applications
- Inflammatory Biomarkers in Disease Prognosis
- Plant Molecular Biology Research
Foundation for Research and Technology Hellas
2023-2025
University of Crete
2025
Hospital for Sick Children
2025
Alexander Fleming Biomedical Sciences Research Center
2017-2024
FORTH Institute of Molecular Biology and Biotechnology
2022-2024
Academy of Athens
2011-2016
Kellogg's (Canada)
2011
University of Portsmouth
2004-2006
University of Nottingham
2006
Synovial fibroblasts (SFs) are specialized cells of the synovium that provide nutrients and lubricants for proper function diarthrodial joints. Recent evidence appreciates contribution SF heterogeneity in arthritic pathologies. However, normal profiles molecular networks govern transition from homeostatic to remain poorly defined.
Complex molecular responses preserve gene expression accuracy and genome integrity in the face of environmental perturbations. Here we report that, response to UV irradiation, RNA polymerase II (RNAPII) molecules are dynamically synchronously released from promoter-proximal regions into elongation promote uniform accelerated surveillance whole transcribed genome. The maximised influx de novo RNAPII correlates with increased damage-sensing, as confirmed by progressive accumulation at...
The histone variant macroH2A (mH2A) has been implicated in transcriptional repression, but the molecular mechanisms that contribute to global mH2A-dependent genome regulation remain elusive. Using chromatin immunoprecipitation sequencing (ChIP-seq) coupled with profiling mH2A knockdown cells, we demonstrate singular nucleosomes occupy transcription start sites of subsets both expressed and repressed genes, opposing regulatory consequences. Specifically, mask repressor binding genes activator...
Promoting adaptive immunity with ICIs has drastically improved melanoma prognosis, but not for all patients. Some cases relapse in the first few months, while others keep durable benefit, even after immunotherapy discontinuation. To identify cellular/molecular signatures peripheral blood that could differentiate advanced from metastatic and predict dynamics primary/secondary immune escape, we examined 100 consecutive patients stage III/IV scheduled to start ICIs. At diagnosis, a...
The conjugation of thermoresponsive polymers to multisubunit, multifunctional hybrid type 1 DNA restriction−modification (R−M) enzymes enables temperature-controlled "switching" methylation by the conjugate. Polymers attached enzyme at a subunit distal allow retention recognition and ATPase activity while controlling plasmid DNA. This regulation arises from coil−globule phase transitions polymer as shown in light scattering gel retardation assays.
Abstract Inhibition of transcription caused by DNA damage-impaired RNA polymerase II (Pol II) elongation conceals a local increase in de novo transcription, slowly progressing from Transcription Start Sites (TSSs) to gene ends. Although associated with accelerated repair Pol II-encountered lesions and limited mutagenesis, it is still unclear how this mechanism maintained during genotoxic stress-recovery. Here we uncover widespread gain chromatin accessibility preservation the active H3K27ac...
Abstract Background Neural stem cell (NSC) proliferation and differentiation in the mammalian brain decreases to minimal levels postnatally. Nevertheless, neurogenic niches persist adult cortex hippocampus rodents, primates humans, with NSC sharing key regulatory mechanisms development. Adult neurogenesis impairments have been linked Alzheimer’s disease (AD) pathology. Addressing these by using neurotrophic factors is a promising new avenue for therapeutic intervention based on neurogenesis....
Antitumor immune surveillance is the key feature of tumour progression and response to treatment in various malignancies, such as lymphomas. Myeloid derived suppressor cells (MDSCs) are bone marrow (BM)-derived with potent suppressive properties, implicated T cell inhibition dissemination. In Diffuse Large B-cell Lymphoma (DLBCL), circulating MDSCs constitute immunosuppressive tumor microenvironment, while contribution BM disease pathogenesis remains elusive. present study we aimed evaluate...
Abstract Background Successful non‐viral gene targeting requires vectors to meet two conflicting needs—strong binding protect the genetic material during transit and weak at target site enable release. Responsive polymers could fulfil such requirements through switching of states, e.g. chain‐extended coil chain‐collapsed globule phase transition that occurs a lower critical solution temperature (LCST), in order transport nucleic acid one polymer state release it another. Methods The ability...
STAT5 interacts with other factors to control transcription, and the mechanism of regulation is interest as constitutive active has been reported in malignancies. Here, LSD1 HDAC3 were identified novel STAT5a interacting partners pro-B cells. Characterization STAT5a, target genes by ChIP-seq RNA-seq revealed gene subsets regulated independent or combined action LSD1/HDAC3 play dual role their activation repression. Genes bound alone combination weakly associated enriched for canonical GAS...
Poor nuclear entry, especially into nondividing cells, is a limiting factor in nonviral gene delivery. We have engineered novel chimeric vector relying on the controlled assembly of TAT-tagged multisubunit DNA binding protein (EcoR124I) with expression plasmids containing EcoR124I recognition site. Molecular interactions this molecular were studied by electrophoretic mobility shift assay and atomic force microscopy. Maintenance nanocomplexes an appropriate stoichiometric ratio was both...
Copolymers of N-isopropylacrylamide with a fluorescent probe monomer were grafted to branched poly(ethyleneimine) generate polycations that exhibited lower critical solution temperature (LCST) behavior. The structures these polymers confirmed by spectroscopy, and their phase transitions before after complexation DNA followed using ultraviolet fluorescence spectroscopy light scattering. Interactions investigated ethidium bromide displacement assays, while temperature-induced changes in...
Viroids are small circular RNAs infecting a wide range of plants. They do not code for any protein or peptide and therefore rely on their structure biological cycle. Observed phenotypes viroid infected plants thought to occur through changes at the transcriptional/translational level host. A mechanism involved in such is RNA-directed DNA methylation (RdDM). Till today, there contradictory works about viroids interference RdDM. In this study, we investigated epigenetic effect infection...
Abstract In addition to being essential for gene expression, transcription is crucial the maintenance of genome integrity. Here, we undertook a systematic approach, monitor assembly kinetics pre-initiating RNA Polymerase (Pol) II at promoters steady state and different stages during recovery from UV irradiation-stress, when pre-initiation initiation steps have been suggested be transiently shut down. Taking advantage reversible dissociation Pol after high salt treatment, found that de novo...