A5031789116- Horticultural and Viticultural Research
- Plant Gene Expression Analysis
- Plant biochemistry and biosynthesis
- Fermentation and Sensory Analysis
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant Virus Research Studies
- Photosynthetic Processes and Mechanisms
- Biochemical and biochemical processes
- Plant Stress Responses and Tolerance
- Plant Parasitism and Resistance
- Plant Physiology and Cultivation Studies
- Antioxidant Activity and Oxidative Stress
- Light effects on plants
- Sirtuins and Resveratrol in Medicine
- Livestock and Poultry Management
- Postharvest Quality and Shelf Life Management
- Genomics and Phylogenetic Studies
- Plant and animal studies
- Plant Pathogens and Fungal Diseases
- Phytoplasmas and Hemiptera pathogens
- Pacific and Southeast Asian Studies
- Microbial Metabolism and Applications
- Cocoa and Sweet Potato Agronomy
- Genetic Mapping and Diversity in Plants and Animals
Institute for Integrative Systems Biology
2019-2025
Universitat de València
2019-2025
Center for Research in Agricultural Genomics
2013-2025
Fondazione Edmund Mach
2024
Parc Científic de la Universitat de València
2022
Consejo Superior de Investigaciones Científicas
2014-2021
Universitat Autònoma de Barcelona
2013-2015
Universitat de Barcelona
2015
Pontificia Universidad Católica de Chile
2004-2014
Abstract Background The MYB superfamily constitutes the most abundant group of transcription factors described in plants. Members control processes such as epidermal cell differentiation, stomatal aperture, flavonoid synthesis, cold and drought tolerance pathogen resistance. No genome-wide characterization this family has been conducted a woody species grapevine. In addition, previous analysis recently released grape genome sequence suggested expansion events several gene families involved...
Anthocyanins, flavan-3-ols, and flavonols are the three major classes of flavonoid compounds found in grape berry tissues. Several viticultural practices increase content fruit, but underlying genetic mechanisms responsible for these changes have not been completely deciphered. The impact post-veraison sunlight exposure on anthocyanin flavonol accumulation skin its relation to expression different transcriptional regulators known be involved synthesis was studied. Treatments consisting...
Because of the vast range functions that phenylpropanoids possess, their synthesis requires precise spatiotemporal coordination throughout plant development and in response to environment. The accumulation these secondary metabolites is transcriptionally controlled by positive negative regulators from MYB basic helix-loop-helix protein families. We characterized four grapevine (Vitis vinifera) R2R3-MYB proteins C2 repressor motif clade, all which harbor ethylene factor-associated amphiphilic...
Abstract Background Development of eukaryotic organisms is controlled by transcription factors that trigger specific and global changes in gene expression programs. In plants, MADS-domain act as master regulators developmental switches organ specification. However, the mechanisms which these dynamically regulate their target genes at different stages are still poorly understood. Results We characterized relationship chromatin accessibility, expression, DNA binding two proteins Arabidopsis...
Grapevine is one of the most economically important crops worldwide. However, previous versions grapevine reference genome tipically consist thousands fragments with missing centromeres and telomeres, limiting accessibility repetitive sequences, centromeric telomeric regions, study inheritance agronomic traits in these regions. Here, we assembled a telomere-to-telomere (T2T) gap-free for cultivar PN40024 using PacBio HiFi long reads. The T2T (PN_T2T) 69 Mb longer 9018 more genes identified...
R2R3-MYB transcription factors (TFs) belong to a large and functionally diverse protein superfamily in plants. In this study, we explore the evolution function of family grapevine (Vitis vinifera L.), high-value fruit crop. We identified manually curated 134 genes using RNA-Seq data, named them systematically according Super-Nomenclature Committee. novel genes, splicing variants grapevine/woody-specific duplicated subgroups, suggesting possible neo- sub-functionalization events. Regulatory...
Stilbene synthase (STS) is the key enzyme leading to biosynthesis of resveratrol. Recently we reported two R2R3-MYB transcription factor (TF) genes that regulate stilbene biosynthetic pathway in grapevine: VviMYB14 and VviMYB15. These are strongly co-expressed with STS under a range stress developmental conditions, agreement specific activation promoters by these TFs. Genome-wide gene co-expression analysis using separate transcriptome compendia based on microarray RNA sequencing data...
Grapevine (Vitis vinifera L.) is a species well known for its adaptation to radiation. However, photomorphogenic factors related UV-B responses have not been molecularly characterized. We cloned and studied the role of RECEPTOR (UVR1), ELONGATED HYPOCOTYL 5 (HY5), HY5 HOMOLOGUE (HYH) from V. vinifera. performed gene functional characterizations, generated co-expression networks, tested them in different environmental conditions. These genes complemented Arabidopsis uvr8 hy5 mutants...
Summary Grapevine organs accumulate anthocyanins in a cultivar‐specific and environmentally induced manner. The MYBA 1 ‐ A2 genes within the berry color locus chromosome 2 represent major genetic determinants of fruit color. simultaneous occurrence transposon insertions point mutations these is responsible for most white‐skinned phenotypes; however, red pigmentation found vegetative suggests presence additional regulators. This work describes genomic region 14 containing three closely...
Plastids, the defining organelles of plant cells, undergo physiological and morphological changes to fulfill distinct biological functions. In particular, differentiation chloroplasts into chromoplasts results in an enhanced storage capacity for carotenoids with industrial nutritional value such as beta-carotene (provitamin A). Here, we show that synthetically inducing a burst production phytoene, first committed intermediate carotenoid pathway, elicits artificial chloroplast-to-chromoplast...
The stilbenoid pathway is responsible for the production of resveratrol in grapevine (Vitis vinifera L.). A few transcription factors (TFs) have been identified as regulators this but extent control has not deeply studied. Here we show how DNA affinity purification sequencing (DAP-Seq) allows genome-wide TF-binding site interrogation grape. We obtained 5190 and 4443 binding events assigned to 4041 3626 genes MYB14 MYB15, respectively (approximately 40% peaks located within −10 kb start...
The genome sequence of the diploid and highly homozygous Vitis vinifera genotype PN40024 serves as reference for many grapevine studies. Despite several improvements to assembly, its current version PN12X.v2 is quite fragmented only represents haploid state with mixed haplotypes. In fact, being nearly homozygous, this contains heterozygous regions that are yet be resolved. Taking opportunity long-read sequencing technologies offer fully discriminate haplotype sequences, an improved...
Variegation is a rare type of mosaicism not fully studied in plants, especially fruits. We examined red and white sections grape (Vitis vinifera cv. 'Béquignol') variegated berries found that accumulation products from branches the phenylpropanoid isoprenoid pathways showed an opposite tendency. Light-responsive flavonol monoterpene levels increased anthocyanin-depleted areas correlation with increasing MYB24 expression. Cistrome analysis suggested binds to promoters 22 terpene synthase...
European chickens were introduced into the American continents by Spanish after their arrival in 15th century. However, there is ongoing debate as to presence of pre-Columbian among Amerindians South America, particularly relation Chilean breeds such Araucana and Passion Fowl. To understand origin these populations, we have generated partial mitochondrial DNA control region sequences from 41 native specimens compared them with a previously database ≈1,000 domestic chicken across world well...
Grapevine (Vitis vinifera L.) is a widely cultivated fruit crop whose growth and productivity are greatly affected by low temperatures. On the other hand, wild Vitis species represent valuable genetic resources of natural stress tolerance. We have isolated characterized MYB-like gene encoding putative GARP-type transcription factor from Amur grape (V. amurensis) designated as VaAQUILO. AQUILO (AQ) induced cold in both V. amurensis vinifera, its overexpression results significantly improved...
Representing large biological data as networks is becoming increasingly adopted for predicting gene function while elucidating the multifaceted organization of life processes. In grapevine (Vitis vinifera L.), network analyses have been mostly to contribute understanding regulatory mechanisms that control berry composition. Whereas some studies used co-expression find common pathways and putative targets transcription factors related development metabolism, others defined primary secondary...
Soil flooding is a compound abiotic stress that alters soil properties and limits atmospheric gas diffusion (O 2 CO ) to the roots. The involvement of abscisic acid (ABA) in regulation flooding-specific genetic metabolic responses has been scarcely studied despite its key importance as regulator other conditions. To attain this objective, wild type ABA-deficient tomatoes were subjected short-term (24 h) waterlogging. After period, exchange parameters reduced but not plants always had higher...
Abstract Grapevine (Vitis vinifera L.) is one of the most widely cultivated fruit crops because winemaking industry has huge economic relevance worldwide. Uncovering molecular mechanisms controlling developmental progression plant organs will prove essential for maintaining high-quality grapes, expressly in context climate change, which impairs ripening process. Through a deep inspection transcriptomic data, we identified VviNAC60, member NAC transcription factor family, as putative...
L-serine (Ser) and L-glycine (Gly) are critically important for the overall functioning of primary metabolism. We investigated interaction phosphorylated pathway Ser biosynthesis (PPSB) with photorespiration-associated glycolate (GPSB) using Arabidopsis thaliana PPSB-deficient lines, GPSB-deficient mutants, crosses PPSB GPSB mutants. lines mainly showed retarded root growth. Mutation photorespiratory enzyme Ser-hydroxymethyltransferase 1 (SHMT1) in a background resumed growth induced change...
Multivalent binding allows high selectivity and affinity in a ligand-protein interaction. The N-end rule pathway is ubiquitin (Ub)-dependent proteolytic system which specific E3s, called N-recognins, mediate ubiquitylation through the recognition of types 1 2, destabilizing N-terminal residues substrates. We recently identified set E3 Ub ligases (named UBR1-UBR7) containing 70-residue UBR box, we demonstrated that UBR1, UBR2, UBR4, UBR5 can bind to residues. To explore model heterovalent...
Abstract Background Under drought, plants accumulate the signaling hormone abscisic acid (ABA), which induces rapid closure of stomatal pores to prevent water loss. This event is trigged by a series signals produced inside guard cells finally reduce their turgor. Many these events are tightly regulated at transcriptional level, including control exerted MYB proteins. In previous study, while identifying grapevine R2R3 family, two closely related genes, VvMYB30 and VvMYB60 were found with...