A5087380026- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant Gene Expression Analysis
- Plant Virus Research Studies
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Poxvirus research and outbreaks
- Plant Physiology and Cultivation Studies
- Herpesvirus Infections and Treatments
- Plant nutrient uptake and metabolism
- Toxin Mechanisms and Immunotoxins
- Chromosomal and Genetic Variations
- Plant Genetic and Mutation Studies
- Gene expression and cancer classification
- Genetics, Bioinformatics, and Biomedical Research
- Transgenic Plants and Applications
- Viral Infections and Immunology Research
- Plant and Fungal Interactions Research
- Animal Virus Infections Studies
- Advanced biosensing and bioanalysis techniques
- Genetic Mapping and Diversity in Plants and Animals
- MicroRNA in disease regulation
- Light effects on plants
- Animal Genetics and Reproduction
- Advanced Biosensing Techniques and Applications
Center for Research in Agricultural Genomics
2012-2025
Institució Catalana de Recerca i Estudis Avançats
2011-2025
Consejo Superior de Investigaciones Científicas
1989-2023
Universitat Autònoma de Barcelona
2013-2023
Universitat de Barcelona
2023
California Institute of Technology
2002-2017
Universidade Federal do Rio de Janeiro
2007
Cabot (United States)
2000-2004
Carnegie Institution for Science
2004
Carnegie Department of Plant Biology
2004
The completion of the Arabidopsis thaliana genome sequence allows a comparative analysis transcriptional regulators across three eukaryotic kingdoms. dedicates over 5% its to code for more than 1500 transcription factors, about 45% which are from families specific plants. factors that belong common all eukaryotes do not share significant similarity with those other kingdoms beyond conserved DNA binding domains, many have been arranged in combinations each lineage. genome-wide comparison...
Abstract In plants, low temperature and dehydration activate a set of genes containing C-repeat/dehydration-responsive elements in their promoter. It has been shown previously that the Arabidopsis CBF/DREB1 transcription activators are critical regulators gene expression signal transduction cold acclimation. Here, we report isolation an apparent homolog proteins (CBF4) plays equivalent role during drought adaptation. contrast to three already identified homologs, which induced under stress,...
The MADS domain homeotic proteins APETALA1 (AP1), APETALA3 (AP3), PISTILLATA (PI), and AGAMOUS (AG) act in a combinatorial manner to specify the identity of Arabidopsis floral organs. molecular basis for this mode action was investigated. Immunoprecipitation experiments indicate that all four are capable interacting with each other. However, these exhibit "partner-specificity" formation DNA-binding dimers; only AP1 homodimers, AG AP3/PI heterodimers binding CArG-box sequences. Both...
The MADS-domain transcription factor APETALA1 (AP1) is a key regulator of Arabidopsis flower development. To understand the molecular mechanisms underlying AP1 function, we identified its target genes during floral initiation using combination gene expression profiling and genome-wide binding studies. Many targets encode transcriptional regulators, including known repressors. latter are down-regulated by AP1, suggesting that it initiates development abrogating inhibitory effects these genes....
In many organisms, the circadian clock is composed of functionally coupled morning and evening oscillators. Arabidopsis, oscillator coupling relies on a core loop in which component TIMING OF CAB EXPRESSION 1 (TOC1) was proposed to activate subset morning-expressed genes. Here, we show that TOC1 does not function as an activator but rather general repressor gene expression. Repression occurs through rhythmic association promoters Hormone-dependent induction analysis RNA interference plants...
Epicuticular wax forms a layer of hydrophobic material on plant aerial organs, which constitutes protective barrier between the and its environment. We report here identification WIN1, an Arabidopsis thaliana ethylene response factor-type transcription factor, can activate deposition in overexpressing plants. constitutively expressed WIN1 transgenic plants, found that leaf epidermal accumulation was up to 4.5-fold higher these plants than control A significant increase also stems....
In plants, members of microRNA (miRNA) families are often predicted to target the same or overlapping sets genes. It has thus been hypothesized that these miRNAs may act in a functionally redundant manner. This hypothesis is tested here by studying effects elimination all three MIR164 family from Arabidopsis. was found loss miR164 activity leads severe disruption shoot development, contrast effect mutation any single gene. indicates indeed redundant. Differences expression patterns...
Drought represents a major threat to food security. Mechanistic data describing plant responses drought have been studied extensively and genes conferring resistance introduced into crop plants. However, plants with enhanced usually display lower growth, highlighting the need for strategies uncouple from growth. Here, we show that overexpression of BRL3, vascular-enriched member brassinosteroid receptor family, can confer stress tolerance in Arabidopsis. Whereas loss-of-function mutations...
Abstract Background Development of eukaryotic organisms is controlled by transcription factors that trigger specific and global changes in gene expression programs. In plants, MADS-domain act as master regulators developmental switches organ specification. However, the mechanisms which these dynamically regulate their target genes at different stages are still poorly understood. Results We characterized relationship chromatin accessibility, expression, DNA binding two proteins Arabidopsis...
Abstract The Arabidopsis FLOWERING LOCUS C (FLC) gene is a key floral repressor in the maintenance of vernalization response. In vernalization-sensitive genetic backgrounds, FLC levels are high, and they decline after exposure to long cold periods. Four paralogs (MAF2 [MADS AFFECTING FLOWERING2] MAF5) arranged tandem array on bottom chromosome V. We used reverse genetics approach analyze their functions. Loss-of-function gain-of-function studies indicate that MAF2 acts as repressor....
Abstract The composition and permeability of the cuticle has a large influence on its ability to protect plant against various forms biotic abiotic stress. WAX INDUCER1 (WIN1) related transcription factors have recently been shown trigger wax production, enhance drought tolerance, modulate cuticular when overexpressed in Arabidopsis thaliana. We found that WIN1 influences cutin, polyester backbone cuticle. overexpression induces compositional changes an overall increase cutin production...
The MADS domain proteins APETALA1 (AP1), APETALA3 (AP3), PISTILLATA (PI), and AGAMOUS (AG) specify the identity of Arabidopsis floral organs. API AG homocomplexes AP3-PI heterocomplexes bind to CArG-box sequences. DNA-binding properties these complexes were investigated. We find that API, are all capable recognizing same sites, although with somewhat different affinities. In addition, three induce similar conformational changes on a sequence. Phasing analysis reveals induced distortion is...
We have compared the gene expression profiles of inflorescences floral homeotic mutants apetala1, apetala2, apetala3, pistillata, and agamous with that wild-type plants using a flower-specific cDNA microarray whole genome oligonucleotide array. By combining data sets from individual mutant/wild type comparisons, we were able to identify large number genes are, within flowers, predicted be specifically or at least predominantly expressed in one organ. analyzed patterns several these by situ...
The Arabidopsis FLC gene encodes a MADS domain protein that acts as repressor of flowering. Late-flowering vernalization-responsive ecotypes and mutants have high steady-state levels transcript, which decrease during the promotion flowering by vernalization. Therefore, has central role in regulating response to We isolated an gene, MAF1, is closely related FLC. Overexpression studies demonstrate MAF1 produces comparable effects FLC, likely similar function regulation In contrast however,...
Detailed information about stage-specific changes in gene expression is crucial for the understanding of regulatory networks underlying development.Here, we describe global dynamics during early flower development, a key process life cycle plant, which floral patterning and specification organs established.We used novel induction system Arabidopsis, allows isolation large number synchronized buds, conjunction with whole-genome microarray analysis to identify genes differential at distinct...
Detailed information about stage-specific changes in gene expression is crucial for the understanding of regulatory networks underlying development. Here, we describe global dynamics during early flower development, a key process life cycle plant, which floral patterning and specification organs established. We used novel induction system Arabidopsis, allows isolation large number synchronized buds, conjunction with whole-genome microarray analysis to identify genes differential at distinct...
Most positive strand RNA viruses infecting plants and animals encode proteins containing the so-called nucleotide binding motif (NTBM) (1) in their amino acid sequences (2). As suggested from high level of sequence similarity these viral with recently described superfamilies helicase-like (3–5), NTBM-containing cylindrical inclusion (Cl) protein plum pox virus (PPV), which belongs to potyvirus group viruses, is shown be able unwind duplexes. This activity was found dependent on hydrolysis...