A5033227281- Plant Molecular Biology Research
- Postharvest Quality and Shelf Life Management
- Plant Gene Expression Analysis
- Plant Physiology and Cultivation Studies
- Plant Reproductive Biology
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Light effects on plants
- Plant tissue culture and regeneration
- Plant Virus Research Studies
- Plant Pathogens and Fungal Diseases
- Plant-Microbe Interactions and Immunity
- Plant biochemistry and biosynthesis
- Flowering Plant Growth and Cultivation
- Plant nutrient uptake and metabolism
- Plant and animal studies
- Phytochemicals and Antioxidant Activities
- Polysaccharides and Plant Cell Walls
- Horticultural and Viticultural Research
- Plant responses to water stress
- Plant Surface Properties and Treatments
- Potato Plant Research
- Insect-Plant Interactions and Control
- Banana Cultivation and Research
- Fungal Plant Pathogen Control
United States Department of Agriculture
2016-2025
Agricultural Research Service
2016-2025
University of California, Davis
2016-2025
University of California, Los Angeles
2022
Plant (United States)
2019-2021
Anhui Agricultural University
2018
Agricultural Research Organization
2010
University of Liverpool
2009
Cabot (United States)
2000-2004
Carnegie Institution for Science
2004
The completion of the Arabidopsis thaliana genome sequence allows a comparative analysis transcriptional regulators across three eukaryotic kingdoms. dedicates over 5% its to code for more than 1500 transcription factors, about 45% which are from families specific plants. factors that belong common all eukaryotes do not share significant similarity with those other kingdoms beyond conserved DNA binding domains, many have been arranged in combinations each lineage. genome-wide comparison...
Epicuticular wax forms a layer of hydrophobic material on plant aerial organs, which constitutes protective barrier between the and its environment. We report here identification WIN1, an Arabidopsis thaliana ethylene response factor-type transcription factor, can activate deposition in overexpressing plants. constitutively expressed WIN1 transgenic plants, found that leaf epidermal accumulation was up to 4.5-fold higher these plants than control A significant increase also stems....
Ripening of the model fruit tomato (Solanum lycopersicum) is controlled by a transcription factor network including NAC (NAM, ATAF1/2, and CUC2) domain proteins such as No-ripening (NOR), SlNAC1, SlNAC4, but very little known about targets or how they regulate ripening. Here, we conducted systematic search fruit-expressed genes showed that silencing NOR-like1 (Solyc07g063420) using virus-induced gene (VIGS) inhibited specific aspects initiation was delayed 14 days when function inactivated...
Abstract The tomato non-ripening (nor) mutant generates a truncated 186-amino-acid protein (NOR186) and has been demonstrated previously to be gain-of-function mutant. Here, we provide more evidence support this view answer the open question of whether NAC-NOR gene is important in fruit ripening. Overexpression nor did not restore full ripening phenotype. Further analysis showed that NOR186 located nucleus binds but does activate promoters 1-aminocyclopropane-1-carboxylic acid synthase2...
Abstract The abscission process is initiated by changes in the auxin gradient across zone (AZ) and triggered ethylene. Although gene expression have been correlated with ethylene-mediated execution of abscission, there almost no information on molecular biochemical basis increased AZ sensitivity to We examined transcriptome tomato (Solanum lycopersicum ‘Shiran 1335’) flower during rapid acquisition ethylene following removal, which depletes from auxin, or without preexposure...
Nectaries are secretory organs that widely present in flowering plants function to attract floral pollinators. Owing diversity nectary positions and structures, they thought have originated multiple times during angiosperm evolution, with their potential contribution the diversification of pollinating animals being considerable. We investigated genetic basis diverse forms eudicot species using CRABS CLAW (CRC), a gene required for nectaries Arabidopsis. CRC expression is conserved...
Tomato is considered as the genetic model for climacteric fruits, in which three major players control fruit ripening process: ethylene, transcription factors, and DNA methylation. The fruitENCODE project has now shown that there are multiple transcriptional circuits regulating different species, H3K27me3, instead of methylation, plays a conserved role restricting these pathways. In addition, function core tomato factors being questioned. We have employed CRISPR/Cas9 genome editing to mutate...
Age, as a threshold of floral competence acquisition, prevents precocious flowering when there is insufficient biomass, and ensures independent environmental conditions; however, the underlying regulatory mechanisms are largely unknown. In this study, silencing expression nuclear factor gene, CmNF-YB8, from short day plant chrysanthemum (Chrysanthemum morifolium), results in transition juvenile to adult, well early flowering, regardless length conditions. The SQUAMOSA PROMOTER BINDING-LIKE...
The flesh colour and phenolic metabolism in potato tuber during curing after cut were investigated. Result indicated that postharvest not only changed curing, but also improved fresh-cut for 12 days fresh cut. Significantly lower PAL higher content PPO activities treatment potatoes detected compared to the control, which lead browning slices from treated potatoes. HPLC analysis revealed amounts of total phenolics, chlorogenic acid, gallic acid protocatechuic induced by highly accumulated Our...
Auxin response factors (ARFs) are involved in auxin-mediated transcriptional regulation plants. In this study, we performed functional characterization of SlARF6A tomato. is located the nucleus and exhibits activator activity. Overexpression increased chlorophyll contents fruits leaves tomato plants, whereas downregulation decreased compared with those wild-type (WT) Analysis chloroplasts using transmission electron microscopy indicated sizes SlARF6A-overexpressing plants numbers...
The response of plants to drought stress includes reduced transpiration as stomates close in increased abscisic acid (ABA) concentrations. Constitutive overexpression 9-cis-epoxycarotenoid dioxygenase (NCED), a key enzyme ABA biosynthesis, increases resistance, but causes negative pleiotropic effects on plant growth and development. We overexpressed the tomato NCED (LeNCED1) petunia under control stress-inducible promoter, rd29A. Under water stress, transgenic had transcripts mRNA, elevated...
In many plant species, including rose (Rosa hybrida), flower senescence is promoted by the gaseous hormone ethylene and inhibited cytokinin (CTK) class of hormones. However, molecular mechanisms underlying these antagonistic effects are not well understood. this study, we characterized association between a pathogenesis-related PR-10 family gene from (RhPR10.1) hormonal regulation senescence. Quantitative reverse transcription PCR analysis showed that RhPR10.1 was expressed at high levels...
Summary Chloroplast development and chlorophyll(Chl)metabolism in unripe tomato contribute to the growth quality of fruit, however these mechanisms are poorly understood. In this study, we initially investigated seven homeobox‐containing transcription factors ( TF s) with specific ripening‐associated expression patterns using virus‐induced gene silencing VIGS ) technology found that inhibiting one s, BEL 1‐ LIKE HOMEODOMAIN 11 (Sl 11), significantly increased Chl levels fruit. This enhanced...
The timing of plant organ abscission is modulated by the balance two hormones, ethylene and auxin, while mechanism shedding depends on loss middle lamella pectin in zone (AZ). However, mechanisms involved sensing auxin that affect degradation during are not well understood. In this study, we identified members APETALA2/ethylene-responsive factor (AP2/ERF) transcription family rose (Rosa hybrida), RhERF1 RhERF4 which play a role petal abscission. expression was influenced respectively....
Members of the genus Juglans are monecious wind-pollinated trees in family Juglandaceae with highly heterozygous genomes, which greatly complicates genome sequence assembly. The genomes interspecific hybrids usually comprised haploid parental species. We exploited this attribute to avoid heterozygosity and sequenced an hybrid microcarpa × J. regia using a novel combination single-molecule sequencing optical mapping technologies. resulting assemblies both were remarkably complete including...
Abstract Reactive oxygen species (ROS) are unstable reactive molecules that toxic to cells. Regulation of ROS homeostasis is crucial protect cells from dysfunction, senescence, and death. In plant leaves, mainly generated chloroplasts tightly temporally restricted by the circadian clock. However, little known about how regulated in nonphotosynthetic organs, such as petals. Here, we showed hydrogen peroxide (H2O2) levels exhibit typical rhythmicity rose (Rosa hybrida) petals, consistent with...
Fruit quality is defined by attributes that give value to a commodity. Flavor, texture, nutrition, and shelf life are key traits ensure market consumer acceptance. In pear fruit, soluble sugars, organic acids, amino total flavonoids contribute flavor overall quality. Transcription factors (TFs) regulate the accumulation of these metabolites during development or in response environment. Here, we report novel TF, PpbZIP44, as positive regulator primary secondary metabolism fruit. Analysis...
Summary The immutans ( im ) variegation mutant of Arabidopsis thaliana contains green‐ and white‐sectored leaves due to the action a nuclear recessive gene. mutation is somatically unstable, degree sectoring influenced by light temperature. Whereas cells in green sectors contain normal chloroplasts, white are heteroplastidic non‐pigmented plastids that lack organized lamellar structures, as well small pigmented and/or rare chloroplasts. This indicates not affected equally expression ‘plastid...
UV radiation induces two major classes of pyrimidine dimers: the [6-4] pyrimidone photoproduct (6-4 product) and cyclobutane dimer (CPD). Many organisms produce enzymes, termed photolyases, that specifically bind to these damage products split them via a UV-A/blue light-dependent mechanism, thereby reversing damage. These photolyases are specific for either CPDs or 6-4 products. A gene expresses protein with photolyase activity in vitro was recently cloned from Drosophila melanogaster...
Senescence is a phase of leaf ontogeny marked by declining photosynthetic activity that, in soybean (Glycine max [L.] Merr.), paralleled decline chloroplast function. Soybean leaves have different patterns capacity and function associated with nodal position sink activity. The objective this work was to determine whether from nodes 3 6 soybean, which show these patterns, are similarly regulated respect ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) content also ascertain the...
Multimeric protein complexes in chloroplasts and mitochondria are generally composed of products both nuclear organelle genes the cell. A central problem eukaryotic cell biology is to identify understand molecular mechanisms for integrating production accumulation two separate genomes. Ribulose bisphosphate carboxylase (Rubisco) localized photosynthetic cells small subunits (SS) large (LS) coded by rbcS chloroplast rbcL genes, respectively. Transgenic tobacco plants containing antisense DNA...