A5101736813- Horticultural and Viticultural Research
- Plant biochemistry and biosynthesis
- Plant Gene Expression Analysis
- Fermentation and Sensory Analysis
- Neuroscience and Neuropharmacology Research
- Photoreceptor and optogenetics research
- Ion channel regulation and function
- Nicotinic Acetylcholine Receptors Study
- Plant Physiology and Cultivation Studies
- Biochemical and biochemical processes
- Postharvest Quality and Shelf Life Management
- Antioxidant Activity and Oxidative Stress
- Plant Reproductive Biology
- Microbial Metabolism and Applications
- Motivation and Self-Concept in Sports
- Smart Agriculture and AI
- Social Acceptance of Renewable Energy
- Biochemical effects in animals
- Genetic and Kidney Cyst Diseases
- Light effects on plants
- Evolutionary Algorithms and Applications
- Ion Transport and Channel Regulation
- Phytochemicals and Antioxidant Activities
- Plant Surface Properties and Treatments
- Genetic factors in colorectal cancer
University of Verona
2016-2024
University of Catania
2023-2024
University of Palermo
2020-2021
Tecnologie Avanzate (Italy)
2021
Medway School of Pharmacy
1998-1999
University College London
1992-1996
A small set of TTG2-like homolog proteins from different species belonging to the WRKY family transcription factors were shown share a similar mechanism action and control partially conserved biochemical/developmental processes in their native species. In particular, by activating P-ATPases residing on tonoplast, PH3 Petunia hybrida promotes vacuolar acidification petal epidermal cells whereas TTG2 Arabidopsis thaliana enables accumulation proanthocyanidins seed coat. this work we...
Grapevine (Vitis vinifera L.) is a species well known for its adaptation to radiation. However, photomorphogenic factors related UV-B responses have not been molecularly characterized. We cloned and studied the role of RECEPTOR (UVR1), ELONGATED HYPOCOTYL 5 (HY5), HY5 HOMOLOGUE (HYH) from V. vinifera. performed gene functional characterizations, generated co-expression networks, tested them in different environmental conditions. These genes complemented Arabidopsis uvr8 hy5 mutants...
Variegation is a rare type of mosaicism not fully studied in plants, especially fruits. We examined red and white sections grape (Vitis vinifera cv. 'Béquignol') variegated berries found that accumulation products from branches the phenylpropanoid isoprenoid pathways showed an opposite tendency. Light-responsive flavonol monoterpene levels increased anthocyanin-depleted areas correlation with increasing MYB24 expression. Cistrome analysis suggested binds to promoters 22 terpene synthase...
The molecular events that characterize postripening grapevine berries have rarely been investigated and are poorly defined. In particular, a detailed definition of changes occurring during the postharvest dehydration, process undertaken to make some particularly special wine styles, would be great interest for both winemakers plant biologists. We report an exhaustive survey transcriptomic metabolomic responses in representing six genotypes subjected dehydration under identical controlled...
Summary The accumulation of secondary metabolites and the regulation tissue acidity contribute to important traits grape berry influence plant performance in response abiotic biotic factors. In several species a highly conserved MYB–bHLH– WD ( MBW ) transcriptional regulatory complex controls flavonoid pigment synthesis transport, vacuolar acidification epidermal cells. An additional component, represented by WRKY ‐type transcription factor, physically interacts with increasing expression...
Abstract Grapevine (Vitis vinifera L.) is one of the most widely cultivated fruit crops because winemaking industry has huge economic relevance worldwide. Uncovering molecular mechanisms controlling developmental progression plant organs will prove essential for maintaining high-quality grapes, expressly in context climate change, which impairs ripening process. Through a deep inspection transcriptomic data, we identified VviNAC60, member NAC transcription factor family, as putative...
Abstract Fruit growth and development consist of a continuous succession physical, biochemical, physiological changes driven by genetic program that dynamically responds to environmental cues. Establishing recognizable stages over the whole fruit lifetime represents fundamental requirement for research crop cultivation. This is especially relevant in perennial crops like grapevine (Vitis vinifera L.) scale its across genotypes growing conditions. In this work, molecular-based information...
1. Modulation of GABA(A) receptors by external H(+) was examined in cultured rat sympathetic neurones, and Xenopus laevis oocytes human embryonic kidney (HEK) cells expressing recombinant composed combinations alpha 1, beta 2, gamma 2S delta subunits. 2. Changing the pH from 7.4 reduced GABA‐activated currents neurones. titration GABA‐induced current fitted with a model which predicted that interact two sites (PK(a) values 6.4 7.2). 3. For 1 receptors, low (< 7.4) enhanced responses to...
Effective crop improvement, whether through selective breeding or biotech strategies, is largely dependent on the cumulative knowledge of a species' pangenome and its containing genes. Acquiring this specially challenging in grapevine, one oldest fruit crops grown worldwide, which known to have more than 30,000 Well-established research communities studying model organisms created maintained, public private funds, diverse range online tools databases serving as repositories genomes gene...
CRISPR/Cas9 genome editing technology has revolutionized plant breeding by offering precise and rapid modifications. Traditional methods are often slow imprecise, whereas allows for targeted genetic improvements. Previously, direct delivery of Cas9-single guide RNA (sgRNA) ribonucleoprotein (RNP) complexes to grapevine (Vitis vinifera) protoplasts been demonstrated, but successful regeneration edited into whole plants not achieved. Here, we describe an efficient protocol obtaining...
Terpenes contribute to the characteristic flavor and aroma of grapes their derived products while serving protective roles in plants against radiation, oxidative stress, biotic challenges. The phytohormone methyl jasmonate (MeJA) mediates many these processes enhances terpene content grape berries, but its underlying regulatory mechanism remains unclear. To address this, we treated Vitis vinifera cv. Gamay Fréaux berry cell suspensions with MeJA (100 µM) cyclodextrins (50 mM), generating...
1 The functional role played by the postulated disulphide bridge in γ-aminobutyric acid type A (GABAA) receptors and its susceptibility to oxidation reduction were studied using recombinant (murine receptor subunits expressed human embryonic kidney cells) rat neuronal GABAA conjunction with whole-cell single channel patch-clamp techniques. 2 reducing agent dithiothreitol (DTT) reversibly potentiated GABA-activated responses (IGABA) of α1β1 or α1β2 while oxidizing reagent...
The partial dehydration of grapes after harvest is a traditional practice in several winegrowing regions that leads to the production high quality wines. Postharvest (also known as withering) has significant impact on overall metabolism and physiology berry, yielding final product richer sugars, solutes, aroma compounds. These changes are, at least part, result stress response, which controlled transcriptional level, are highly dependent grape water loss kinetics environmental parameters...
The postharvest dehydration of grape berries allows the concentration sugars and other solutes promotes synthesis metabolites aroma compounds unique to high-quality raisin wines such as passito made in Italy. These dynamic changes are dependent on environmental parameters temperature relative humidity, well endogenous factors berry morphology genotype, but contribution each variable is not understood. Here, we compared subjected natural or accelerated dehydration, latter driven by forced air...
Abstract VviAGL11, the Arabidopsis SEEDSTICK homolog, has been proposed to have a causative role in grapevine stenospermocarpy. An association between mutation coding sequence (CDS) and seedless phenotype was reported, however, no working mechanisms demonstrated yet. We performed deep investigation of full VviAGL11 gene collection varieties belonging several seedlessness classes that revealed three different promoter-CDS combinations. By investigating expression alleles, by evaluating their...
1. The mean intracellular pH in area CA1 of rat hippocampal slices was monitored fluorescently after loading the cells with dye BCECF-AM. 2. Including L-glutamate solution superfusing slice led to becoming more acid. This acidification had a roughly Michaelis-Menten dependence on superfused glutamate concentration half-maximal dose around 200 microM: this value must overestimate at most cells, which will be reduced by uptake. 3. glutamate-evoked not significantly blockers glutamate-gated ion...
Abstract Somatic embryogenesis (SE) represents the most appropriate tool for next-generation breeding methods in woody plants such as grapevine (Vitis vinifera L.). However, this species, SE competence is strongly genotype-dependent and molecular basis of phenomenon poorly understood. We explored genetic epigenetic by profiling transcriptome, epigenome, small RNAome undifferentiated, embryogenic, non-embryogenic callus tissues derived from two genotypes differing SE, Sangiovese Cabernet...
Abstract Manual dexterity is a key skill in motor development. There are conflicting studies on the influence of sports practice this and which type sport trains ability most youth. usually assessed with expensive time-consuming tools not easily available to facilities such as schools or clubs. The aim study was assess differences manual performance between young basketball players, sportsmen, non-sportsmen. A further analyze whether coin rotation task reliable tool for assessing dexterity....
The grapevine (Vitis vinifera L.) is widely cultivated worldwide owing to the substantial commercial value of grapes and other products derived from their processing, wines in particular. characterized by a remarkable phenotypic plasticity within same variety, which shapes final berry quality attributes hence reflecting complex interactions between plant environment leading expression wine typicity. In this study, we explored metabolomic transcriptomic basis Glera, white variety particularly...
1. To investigate the transport of potassium on glutamate uptake carrier, current in isolated retinal Müller cells was monitored by whole‐cell clamping, while measuring changes concentration outside ([K+]o) with an ion‐sensitive microelectrode. 2. Activating led to accumulation cells, consistent hypothesis, based less direct evidence, that carrier transports out cell. 3. The glutamate‐evoked rise [K+]o showed pharmacology and sodium dependence uptake. 4. proportional flowing between 0 ‐80...