A5087159524- Plant biochemistry and biosynthesis
- Fermentation and Sensory Analysis
- Horticultural and Viticultural Research
- Biochemical and biochemical processes
- Plant Gene Expression Analysis
- Fungal Plant Pathogen Control
- Microbial Natural Products and Biosynthesis
- Insect-Plant Interactions and Control
- Food Quality and Safety Studies
- Microbial Metabolic Engineering and Bioproduction
- Insect behavior and control techniques
- Advanced Chemical Sensor Technologies
- Fungal Biology and Applications
- Insect Utilization and Effects
- Sirtuins and Resveratrol in Medicine
- Plant Pathogens and Fungal Diseases
- Olfactory and Sensory Function Studies
- Phytochemicals and Antioxidant Activities
- Medicinal Plant Research
- Essential Oils and Antimicrobial Activity
- Agriculture and Rural Development Research
- Analytical Chemistry and Chromatography
- Plant Virus Research Studies
- Photosynthetic Processes and Mechanisms
- Insect Pest Control Strategies
Institut Français de la Vigne et du Vin
2017-2024
Université de Strasbourg
2012-2023
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2022-2023
Institut National de la Recherche Agronomique
2002-2013
Colmar Inra Research Centre
2012
Centre National de la Recherche Scientifique
2008
Laboratoire Reproduction et Développement des Plantes
2008
École Normale Supérieure de Lyon
2008
Université Claude Bernard Lyon 1
2008
The scent of roses (Rosa x hybrida) is composed hundreds volatile molecules. Monoterpenes represent up to 70% percent the content in some cultivars, such as Papa Meilland rose. Monoterpene biosynthesis plants relies on plastid-localized terpene synthases. Combining transcriptomic and genetic approaches, we show that Nudix hydrolase RhNUDX1, localized cytoplasm, part a pathway for free monoterpene alcohols contribute fragrance roses. RhNUDX1 protein shows geranyl diphosphate...
Stilbenes are considered the most important phytoalexin group in grapevine (Vitis vinifera) and they known to contribute protection against various pathogens. The main stilbenes resveratrol its derivatives and, among these, pterostilbene has recently attracted much attention due both antifungal pharmacological properties. Indeed, is 5 10 times more fungitoxic than vitro recent studies have shown that exhibits anticancer, hypolipidemic, antidiabetic A candidate gene approach was used identify...
Terpenoids are one of the largest and most diverse families natural compounds. They heavily used in industry, trend is toward engineering modified microorganisms that produce high levels specific terpenoids. Most studies have focused on creating heterologous pathways for sesquiterpenes Escherichia coli or yeast. We subjected Saccharomyces cerevisiae ERG20 gene (encoding farnesyl diphosphate synthase) to a set amino acid mutations catalytic site at position K197. Mutated strains been shown...
The cytochrome P450 family encompasses the largest of enzymes in plant metabolism, and functions many its members Arabidopsis thaliana are still unknown. Gene coexpression analysis pointed to two P450s that were coexpressed with monoterpene synthases flowers thus predicted be involved monoterpenoid metabolism. We show all four selected genes, terpene (TPS10 TPS14) (CYP71B31 CYP76C3), simultaneously expressed at anthesis, mainly upper anther filaments petals. Upon transient expression...
Variegation is a rare type of mosaicism not fully studied in plants, especially fruits. We examined red and white sections grape (Vitis vinifera cv. 'Béquignol') variegated berries found that accumulation products from branches the phenylpropanoid isoprenoid pathways showed an opposite tendency. Light-responsive flavonol monoterpene levels increased anthocyanin-depleted areas correlation with increasing MYB24 expression. Cistrome analysis suggested binds to promoters 22 terpene synthase...
Methoxypyrazines (MPs) are strongly odorant volatile molecules with vegetable-like fragrances that widespread in plants. Some grapevine (Vitis vinifera) varieties accumulate significant amounts of MPs, including 2-methoxy-3-isobutylpyrazine (IBMP), which is the major MP grape berries. MPs particular importance white Sauvignon Blanc wines. The typicality these wines relies on a fine balance between pea pod, capsicum character and passion fruit/grapefruit due to thiols. Although play crucial...
Wine aroma results from the combination of numerous volatile compounds, some produced by yeast and others in grapes further metabolized yeast. However, little is known about consequences genetic variation on production these metabolites, or metabolic pathways involved metabolism grape compounds. As a tool to decipher how wine develops, we analyzed, under two experimental conditions, 44 compounds population 30 segregants cross between laboratory strain an industrial genotyped at high...
Backgrounds and Aims: In order to assess the suitability of some genotypes for functional genomics studies on terpenol synthesis in grapevine, two pairs cultivars differing their aromatic pattern were compared: Gewurztraminer Savagnin Rose clones Chardonnay (76 809). Methods Results: The genetic analysis based microsatellite markers showed that derived from a single mother plant. same result was found 76 809. Analyses wines, musts berries linalool geraniol present only at very low...
Abstract Alternaria Leaf Blight (ALB), caused by the fungus dauci , is most damaging foliar disease affecting carrots ( Daucus carota ). In order to identify compounds potentially linked resistance A. we have used a combination of targeted and non-targeted metabolomics compare leaf metabolome four carrot genotypes with different levels. Targeted analyses were focused on terpene volatiles, while total methanolic extracts subjected using liquid chromatography couple high-resolution mass...
Aphids are important pests which cause direct damage by feeding or indirect prejudice transmitting plant viruses. Viruses known to induce modifications of cues in ways that can alter vector behavior and virus transmission. In this work, we addressed whether the induced aphid-transmitted Turnip yellows (TuYV) model Arabidopsis thaliana also apply cultivated Camelina sativa, both belonging Brassicaceae family. most experiments, observed a significant increase relative emission volatiles from...
Most phytophagous insect species exhibit a limited diet breadth and specialize on few or single host plant. In contrast, some display remarkably large breadth, with plants spanning several families many species. It is unclear, however, whether this phylogenetic generalism supported by generic metabolic use of common chemical compounds (‘metabolic generalism’) alternatively distinct uses diet-specific (‘multi-host specialism’)? Here, we simultaneously investigated the metabolomes fruit diets...
Fatty acid hydroperoxides can generate short-chained volatile aldehydes that may participate in plant defence. A grapevine hydroperoxide lyase (VvHPL1) clustering to the CYP74B class was functionally characterized with respect a role In leaves, transcripts of this gene accumulated rapidly high abundance response wounding. Cellular functions VvHPL1 were investigated upon heterologous expression tobacco BY-2 cells. C-terminal green fluorescent protein (GFP) fusion located plastids. The...
Alternaria leaf blight, caused by the fungus dauci, is most damaging foliar disease of carrot. Some carrot genotypes exhibit partial resistance to this pathogen and Quantitative Trait Loci (rQTL) have been identified. Co-localization metabolic QTL rQTL identified camphene, α-pinene, α-bisabolene, β-cubebene, caryophyllene, germacrene D α-humulene as terpenes potentially involved in against ALB. By combining genomic transcriptomic analyses, we identified, under co-localization regions,...
Terpenes are one of the largest and most diversified families natural compounds. Although they have found numerous industrial applications, molecular basis their synthesis in plants has, until now, not been fully understood. Plant genomes shown to contain dozens terpene synthase (TPS) genes, however knowledge amino-acidic protein sequence sufficient predict which terpene(s) will be produced by a particular enzyme. In order investigate structural TPS specificity, we performed site directed...
The quality of wine largely depends on aroma perception, but the genetic basis explaining variations compound concentrations in wines is still fragmentary. To unravel links between and hundreds genotypes, we developed a small-scale, high-throughput test-tube vinification (TTV) method capable producing white that reveal potential at scale single vine stock. We evaluated this commercial grapevine varieties (Riesling, Gewurztraminer, Chardonnay, Chasselas, Floreal, Muscat à petits grains...