- Horticultural and Viticultural Research
- Fermentation and Sensory Analysis
- Plant nutrient uptake and metabolism
- Wine Industry and Tourism
- Plant Virus Research Studies
- Chromosomal and Genetic Variations
- Plant and Fungal Interactions Research
- Plant Disease Resistance and Genetics
- Plant tissue culture and regeneration
- Plant Physiology and Cultivation Studies
- Nuts composition and effects
- Agriculture and Rural Development Research
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- Potato Plant Research
- Yeasts and Rust Fungi Studies
- Plant Micronutrient Interactions and Effects
- Legume Nitrogen Fixing Symbiosis
- Plant-Microbe Interactions and Immunity
- Phytoplasmas and Hemiptera pathogens
- Plant Genetic and Mutation Studies
- Plant Pathogens and Fungal Diseases
- Insect Resistance and Genetics
- Insect-Plant Interactions and Control
- Plant pathogens and resistance mechanisms
Institut Français de la Vigne et du Vin
2021
Colmar Inra Research Centre
2003-2015
Université de Strasbourg
2008-2015
Institut National de la Recherche Agronomique
1991-2010
Eurométropole de Strasbourg
2008
Instituto de Investigaciones Agropecuarias
2006
Laboratoire de Biologie et Modélisation de la Cellule
1987-1992
Laboratoire de Génétique Cellulaire
1988-1991
Université Claude Bernard Lyon 1
1987
Abstract The domestication of the Eurasian grape ( Vitis vinifera ssp. sativa ) from its wild ancestor sylvestris has long been claimed to have occurred in Transcaucasia where greatest genetic diversity is found and very early archaeological evidence, including pips artefacts a ‘wine culture’, excavated. Whether or nearby Taurus Zagros Mountains, it hypothesized that this wine culture spread southwards eventually westwards around Mediterranean basin, together with transplantation cultivated...
Vitis vinifera 'Pinot' clones were analysed at 50 microsatellite loci to assess intravarietal genetic diversity. When analysing leaf tissue DNAs, polymorphism mainly resulted from the appearance of a third allele when two expected for heterozygous in diploid species. The sequencing three alleles has confirmed their simultaneous presence tissues. A hypothesis explaining triallelic profiles locus is periclinal chimera meristem structure, which genetically different cell layers coexist....
Previous studies have revealed a wide-spread occurence of the partial and complete genomes reverse-transcribing pararetroviruses in nuclear herbaceous plants. Although absence virus-encoded integrases attests to random incidental incorporation viral sequences, their presence could functional implications for virus-host interactions.
Clonal polymorphism mainly results from somatic mutations that occur naturally during plant growth. In grapevine, arrays of clones have been selected within varieties as a valuable source diversity, among them showing berry color polymorphism. To identify responsible for this polymorphism, we studied collection 33 Pinot noir, gris, and blanc. Haplotypes the L2 cell layer nine were resolved by genotyping self-progenies with molecular markers along 10.07 Mb region chromosome 2, including...
Intravarietal genetic diversification associated with geographical dispersal of a vegetatively propagated species was studied using grapevine Vitis vinifera L. ‘Cabernet Sauvignon’ as model. Fifty-nine clonal samples obtained from 7 countries (France, Chile, Spain, Australia, Hungary, USA, and Italy) were analyzed 84 microsatellite markers. Eighteen polymorphic loci (21.4%) detected, finding 22 different genotypes in the population similarity over 97%. The presence chimeric clones evidenced...
Backgrounds and Aims: In order to assess the suitability of some genotypes for functional genomics studies on terpenol synthesis in grapevine, two pairs cultivars differing their aromatic pattern were compared: Gewurztraminer Savagnin Rose clones Chardonnay (76 809). Methods Results: The genetic analysis based microsatellite markers showed that derived from a single mother plant. same result was found 76 809. Analyses wines, musts berries linalool geraniol present only at very low...
Retrotransposons make a significant contribution to the size, organization and genetic diversity of their host genomes. To characterize retrotransposon families in grapevine genome (the fourth crop plant sequenced) we have combined two approaches: PCR-based method for isolation RnaseH-LTR sequences with computer-based sequence similarity search whole-genome PN40024.Supported by phylogenic analysis, ten novel Ty1/copia were distinguished this study. select canonical reference element from...
Abstract Bulked segregant analysis was employed to identify random amplified polymorphic DNA (RAPD) markers linked a gene that confers rhizomania resistance sugar beet line created from Holly Sugar Company breeding population (USA). Polymorphism revealed with 160 arbitrary 10‐mer oligonucleotide primers screened in two bulks produced by separately pooling the individual DNAs six most resistant and susceptible plants of an F 2 segregating for resistance. A study individuals showed 19...
Sixty-five Nicotiana plumbaginifolia mutants affected in the nitrate reductase structural gene (nia mutants) have been analyzed and classified. The properties evaluated were: (a) enzyme-linked immunosorbent assay (two-site ELISA) using a monoclonal antibody as coating reagent (b) presence of partial catalytic activities, namely reduction with artificial electron donors (reduced methyl viologen, reduced flavin mononucleotide, or bromphenol blue), cytochrome c (Cyt c) NADH. Four classes...
Abstract Intragenic complementation has been observed between apoenzyme nitrate reductase-deficient mutants (nia) of Nicotiana plumbaginifolia. In vivo as in vitro, the NADH-nitrate reductase (NR) activity plants heterozygous for two different nia alleles was lower than wild type plant, but were able to grow on a sole nitrogen source. NR activity, absent extracts homozygous restored by mixing from complementing mutants. These observations suggest that intragenic results either formation...
Molecular markers, based on DNA polymorphisms, are useful tools for identifying individuals, establishing phylogenetic relationships, managing collections of genetic material or assisting breeding. In the present study, we developed a marker set to differentiate Vitis species, grapevine varieties clones belonging same variety. This novel combines, in four PCR amplifications, presence/absence remarkable retrotransposon, Tvv1-Δ3460, inserted at its single locus and SSR polymorphism within two...
<p style="text-align: justify;">We assessed the discriminative power of two nuclear microsatellite (nSSR, simple sequence repeated) loci, ssrVvUCH12 and ssrVvUCH29, which had been suspected to be highly polymorphic display specific alleles in frame a previous characterization work. As more genotyping plant material at higher number nSSR loci was needed for an extensive comparison, one hundred three accessions <em>Vitis vinifera</em>, other <em>Vitis</em>...