- Horticultural and Viticultural Research
- Fermentation and Sensory Analysis
- Plant Physiology and Cultivation Studies
- Plant Gene Expression Analysis
- Plant Pathogens and Fungal Diseases
- Plant biochemistry and biosynthesis
- Plant responses to elevated CO2
- Wine Industry and Tourism
- Forest Insect Ecology and Management
- Phytochemicals and Antioxidant Activities
- Postharvest Quality and Shelf Life Management
- Lipid metabolism and biosynthesis
- Plant and animal studies
- Plant nutrient uptake and metabolism
- Nuts composition and effects
- Photosynthetic Processes and Mechanisms
- Plant-Microbe Interactions and Immunity
- Plant Reproductive Biology
- Plant Stress Responses and Tolerance
- Insect-Plant Interactions and Control
- Biochemical and biochemical processes
- Yeasts and Rust Fungi Studies
- Mycotoxins in Agriculture and Food
- Light effects on plants
- Phytochemical compounds biological activities
Ecophysiologie et Génomique Fonctionnelle de la Vigne
2015-2025
Université de Bordeaux
2014-2024
Unité de Recherche Œnologie
2013-2024
École Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine
2016-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2013-2024
Institut National Polytechnique de Toulouse
2011
Ministère des Transports
2003-2007
Université de Poitiers
2004-2007
Centre National de la Recherche Scientifique
2001-2007
Yale University
2003
Abstract Several lines of evidence suggest that regulation intracellular Ca2+ levels is crucial for adaptation plants to environmental stress. We have cloned and characterized Arabidopsis auto-inhibited Ca2+-ATPase, isoform 4 (ACA4), a calmodulin-regulated Ca2+-ATPase. Confocal laser scanning data green fluorescent protein-tagged version ACA4 as well western-blot analysis microsomal fractions obtained from two-phase partitioning Suc density gradient centrifugation localized small vacuoles....
Grape berry development and ripening are under complex regulation by the nutrients, hormones, environment cues sensed berry. However, biochemical molecular mechanisms underlying these types of poorly understood. A simplified but realistic model system that enables fruit growth conditions to be modulated easily will facilitate deciphering mechanisms. Here, an in vitro culture intact detached grape berries was developed coupling production greenhouse fruiting-cuttings organ techniques. 13C 15N...
Changes in carbohydrate metabolism during grape berry development play a central role shaping the final composition of fruit. The present work aimed to identify metabolic switches and provide insights into timing developmental regulation metabolism. Metabolites from carbon were measured using high-pressure anion-exchange chromatography coupled tandem mass spectrometry enzymatic assays berries either field-grown vines or fruiting cuttings grown greenhouse. Principal component analysis readily...
Reproductive development of grapevine and berry composition are both strongly influenced by temperature. To date, the molecular mechanisms involved in berries response to high temperatures poorly understood. Unlike recent data that addressed effects on elevated applied at whole plant level, present work particularly focuses fruit responses triggered direct exposure heat treatment (HT). In context climate change, this focusing temperature effect microclimate level is particular interest as it...
UV-B radiation and water deficit may trigger flavonol anthocyanin biosynthesis in plant tissues. In addition, previous research has showed strong qualitative effects on grape berry skin profiles response to deficit. The aim of this study is identify the mechanisms leading quantitative changes profiles, separate combined Grapevines (Vitis vinifera L. cv. Tempranillo) were exposed three levels (0, 5.98 9.66 kJ m−2 day−1) subjected two regimes. A effect was found, resulting an increased...
In recent decades, mean global temperatures have increased in parallel with a sharp rise atmospheric carbon dioxide (CO2) levels, apparent implications for precipitation patterns. The aim of the present work is to assess sensitivity different phenological stages grapevine temperature and study influence other factors related climate change (water availability CO2 concentration) on this relationship. Grapevine records from 9 plantings between 42.75°N 46.03°N consisting dates budburst,...
Terroir is about the link between wine and its origin. It has long been understood by sensory evaluation that taste of from a given variety can be related to origins. Specific organoleptic characteristics are influenced environmental factors such as soil climate. By deconstructing effect measurable climate parameters on grape aroma compounds, terroir typicity better understood. Climate influences vine development ripening mainly associated with temperature, radiation rainfall, while...
The lipid composition of membranes is a key determinant for cold tolerance, and enzymes that modify membrane structure seem to be important low-temperature acclimation. We have characterized ALA1 (for aminophospholipid ATPase1), novel P-type ATPase in Arabidopsis belongs the gene family ALA11. deduced amino acid sequence homologous with those yeast DRS2 bovine II, both which are putative translocases. complements deficiency phosphatidylserine internalization into intact cells exhibited by...
Plant lipid transfer proteins (LTPs) are small, cysteine-rich secreted into the extracellular space. They belong to pathogenesis-related (PR-14) family and believed be involved in several physiological processes including plant disease resistance, although their precise biological function is still unknown. Here, we show that a recombinant tobacco LTP1 able load fatty acids jasmonic acid. This binds specific plasma membrane sites, previously characterized as elicitin receptors, shown...
Among various environmental factors, temperature is a major regulator affecting plant growth, development and fruit composition. Grapevine the most cultivated throughout world, grapes are used for wine production human consumption. The molecular mechanisms involved in grapevine tolerance to high temperature, especially at level, poorly understood. To better characterize sensitivity of berries microenvironment, conditions were locally applied Vitis vinifera Cabernet Sauvignon clusters. Two...
Methoxypyrazines (MPs) are strongly odorant volatile molecules with vegetable-like fragrances that widespread in plants. Some grapevine (Vitis vinifera) varieties accumulate significant amounts of MPs, including 2-methoxy-3-isobutylpyrazine (IBMP), which is the major MP grape berries. MPs particular importance white Sauvignon Blanc wines. The typicality these wines relies on a fine balance between pea pod, capsicum character and passion fruit/grapefruit due to thiols. Although play crucial...
We present stilbenoid profiles of canes from 16 grapevines. Fifteen stilbenoids were obtained through isolation and structure identification using MS, NMR, [α](D) or as commercial standards. An HPLC-UV method for the simultaneous quantification nine these was developed applied to Vitis amurensis, arizonica, berlandieri, betulifolia, cinerea, × champini, doaniana, labrusca, candicans (syn. mustangensis), riparia, rupestris, vinifera, Muscadinia rotundifolia, a V. vinifera M. rotundifolia...
: The present work investigates the interactions between soil content, rootstock and scion by focusing on effects of roostocks nitrogen supply grape berry content. Scions Cabernet Sauvignon (CS) Pinot Noir (PN) varieties were grafted either Riparia Gloire de Montpellier (RGM) or 110 Richter (110R) rootstock. 4 rooststock/scion combinations fertilized with 3 different levels after fruit set. Both in 2013 2014, N increased uptake plants, content both vegetative reproductory organs. Rootstock,...
The development of fleshy fruits involves complex physiological and biochemical changes. After fertilization, fruit growth usually begins with cell division, continues both division expansion, allowing set to occur, ends expansion only. In spite the economical importance grapevine, molecular mechanisms controlling berry are not fully understood. present work identified characterized Vitis vinifera elongation bHLH protein (VvCEB1), a basic helix–loop–helix (bHLH) transcription factor in...
The soluble sugar concentration of fleshy fruit is a key determinant quality. It affects directly the sweetness fresh fruits and indirectly properties processed products (e.g. alcohol content in wine). Despite considerable divergence among species, accumulation results from complex interplay three main processes, namely import, metabolism, water dilution. Therefore, inter-species comparison would help to identify common and/or species-specific modes regulation accumulation. For this purpose,...