A5084930157- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Resistance
- Plant Pathogens and Fungal Diseases
- Plant Virus Research Studies
- Plant Parasitism and Resistance
- Transgenic Plants and Applications
- Plant Pathogenic Bacteria Studies
- Legume Nitrogen Fixing Symbiosis
- Plant Reproductive Biology
- Agriculture, Plant Science, Crop Management
- Toxin Mechanisms and Immunotoxins
- Plant tissue culture and regeneration
- Plant pathogens and resistance mechanisms
- Phytochemical compounds biological activities
- Fungal Biology and Applications
- Lipid Membrane Structure and Behavior
- Nematode management and characterization studies
- Plant chemical constituents analysis
- Potato Plant Research
- Microbial Metabolites in Food Biotechnology
- Insect and Pesticide Research
- Protist diversity and phylogeny
- Mycorrhizal Fungi and Plant Interactions
- Insect Pest Control Strategies
- Fungal Plant Pathogen Control
Université Côte d'Azur
2006-2023
Centre National de la Recherche Scientifique
2004-2023
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2021-2023
Institut Sophia Agrobiotech
2012-2023
Institut National de la Recherche Agronomique
1991-2011
Centre National pour la Recherche Scientifique et Technique (CNRST)
2011
Laboratoire des Interactions Plantes Micro-Organismes
1999-2007
Masaryk University
2005
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2004
CEA Paris-Saclay
2004
The rapid and effective activation of disease resistance responses is essential for plant defense against pathogen attack. These are initiated when pathogen-derived molecules (elicitors) recognized by the host. We have developed a strategy creating novel traits whereby transgenic plants respond to infection virulent with production an elicitor. To this end, we generated tobacco harboring fusion between pathogen-inducible hsr 203J gene promoter Phytophthora cryptogea encoding highly active...
Lipid transfer proteins (LTPs) and elicitins are both able to load lipidic molecules share some structural functional properties. While known as elicitors of plant defence mechanisms, the biological function LTP is still an enigma. We show that a wheat LTP1 binds with high affinity sites. Binding in vivo competition experiments point out these binding sites common confirm they receptors elicitins. A mathematical analysis suggests could be represented by allosteric model corresponding...
Plant lipid transfer proteins (LTPs) are small, cysteine-rich secreted into the extracellular space. They belong to pathogenesis-related (PR-14) family and believed be involved in several physiological processes including plant disease resistance, although their precise biological function is still unknown. Here, we show that a recombinant tobacco LTP1 able load fatty acids jasmonic acid. This binds specific plasma membrane sites, previously characterized as elicitin receptors, shown...
Lignin is incorporated into plant cell walls to maintain architecture and ensure long-distance water transport. composition affects the industrial value of material for forage, wood paper production, biofuel technologies. Industrial demands have resulted in an increase use genetic engineering modify lignified wall composition. However, interaction resulting plants with environment must be analyzed carefully that there are no undesirable side effects lignin modification. We show here...
Summary Pathogenic oomycetes have evolved RXLR effectors to thwart plant defense mechanisms and invade host tissues. We analysed the function of one these (Penetration‐Specific Effector 1 ( PSE 1)) whose transcript is transiently accumulated during penetration roots by oomycete P hytophthora parasitica . Expression protein in tobacco Nicotiana tabacum benthamiana ) leaves A rabidopsis thaliana plants was used assess role this effector physiology interactions with pathogens. pharmacological...
Abstract A low-molecular weight protein, termed oligandrin, was purified to homogeneity from the culture filtrate of mycoparasitic fungusPythium oligandrum. When applied decapitated tomato (Lycopersicon esculentum Mill. var. Prisca) plants, this protein displayed ability induce plant defense reactions that contributed restrict stem cell invasion by pathogenic fungus Phytophthora parasitica. According its N-terminal sequence, weight, acidic isoelectric point, ultraviolet spectrum, and...
Elicitins secreted by phytopathogenic Phytophthoraspp. are proteinaceous elicitors of plant defense mechanisms and were demonstrated to load, carry, transfer sterols between membranes. The link elicitor sterol-loading properties was assessed with the use site-directed mutagenesis 47 87 cryptogein tyrosine residues, postulated be involved in sterol binding. Mutated cryptogeins tested for their ability load sterols, bind plasma membrane putative receptors, trigger biological responses. For...
Abstract The hypersensitive response and systemic acquired resistance (SAR) can be induced in tobacco (Nicotiana tabacum L.) plants by cryptogein, an elicitin secreted Phytophthora cryptogea. Stem application of cryptogein leads to the establishment subsequent leaf infection with parasitica var nicotianae, agent black shank disease. We have studied early events that occur after show here a gene encoding extracellular S-like RNase NE is expressed inoculation pathogenic fungus. Upon induction...
Vertebrate females transfer antibodies via the placenta, colostrum and milk or egg yolk to protect their immunologically immature offspring against pathogens. This evolutionarily important of immunity is poorly documented in invertebrates basic questions remain regarding nature extent parental protection offspring. In this study, we show that a lipopolysaccharide binding protein/bactericidal permeability increasing protein family member from invertebrate Biomphalaria glabrata (BgLBP/BPI1)...
Cryptogein is a protein secreted by the phytopathogenic pseudo‐fungus, Phytophthora cryptogea . It basic 10 kDa hydrophilic having hydrophobic pocket and three disulfide bridges. These common features with sterol carrier proteins led us to investigate its possible transfer activity using fluorescent sterol, dehydroergosterol. The results show that cryptogein has one binding site strong affinity for Moreover, this catalyzes of sterols between phospholipidic artificial membranes. This first...
The nematotoxic activity, efficient against plant-parasitic species, found in the culture filtrates of two Hyphomycetes: Paecilomyces lilacinus (nematophagous fungus) and Trichoderma longibrachiatum was characterized. Purification performed by distillation liquid chromatography identification gas-chromatography coupled with a DCI system (dynamic headspace). active substance has been identified as acetic acid which, produced abundantly during fungal growth medium (up to 0.044 mols CH 3 COOH...
The class 1 pathogenesis-related (PR) proteins are thought to be involved in plant defence responses, but their molecular functions unknown. function of PR-1 was investigated tobacco by generating stable PR-1a-silenced lines which other acidic genes (PR-1b and PR-1c) were silenced. Plants lacking extracellular PR-1s more susceptible than wild-type plants the oomycete Phytophthora parasitica displayed unaffected systemic acquired resistance developmental this pathogen. Treatment with...
Cryptogein is a 98-amino acid proteinaceous elicitor of tobacco defense reactions. Specific binding cryptogein to high affinity sites on plasma membranes has been previously reported (K(d) = 2 nM; number sites: 220 fmol/mg protein). In this study, biochemical characterization reveals that they correspond membrane glycoprotein(s) with an N-linked carbohydrate moiety, which involved in binding. Radiation inactivation experiments performed preparations indicated bound specifically component...
ABSTRACT Oxylipins, derived from fatty acid hydroperoxides (FAHs), are thought to play different roles during plant pathogen interactions. During hypersensitive response (HR) some of them serve as signals necessary for defence gene activation whereas others could contribute killing or participate in the execution programmed cell death (PCD) associated with this resistance. In order address role these compounds latter process, we have closely observed lipid peroxidation, first step metabolic...
Ribonuclease (RNase) NE gene expression is induced in tobacco leaves response to Phytophthora parasitica. Using antibodies directed against RNase NE, we demonstrate that extracellular at the early steps of interaction, while fungal tip growth initiated apoplastic compartment. After production Pichia pastoris and biochemical purification, show S-like inhibits hyphal from P. parasitica zoospores Fusarium oxysporum conidia vitro. Conversion into an enzymatically inactive form after mutagenesis...
Oomycetes are a group of filamentous microorganisms that includes both animal and plant pathogens causes major agricultural losses. Phytophthora species can infect most crops plants from natural ecosystems. Despite their tremendous economic ecologic importance, few effective methods exist for limiting the damage caused by these species. New solutions required, development will require improvements in our understanding molecular events governing infection pathogens. In this study, we...