A5016394714- Insect and Pesticide Research
- Insect and Arachnid Ecology and Behavior
- Plant and animal studies
- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Resistance
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogenic Bacteria Studies
- Polysaccharides and Plant Cell Walls
- Plant pathogens and resistance mechanisms
- Plant Parasitism and Resistance
- Plant Pathogens and Fungal Diseases
- Plant Disease Resistance and Genetics
- Insect Pest Control Strategies
- Plant responses to elevated CO2
- Plant Reproductive Biology
- Banana Cultivation and Research
- Vector-Borne Animal Diseases
- Animal Disease Management and Epidemiology
- Viral Infections and Immunology Research
- Plant tissue culture and regeneration
- Plant Virus Research Studies
Fondation Sophia Antipolis
2021-2024
Universidad Politécnica de Madrid
2021
Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail
2014-2020
Institut National de la Recherche Agronomique
2005-2011
Centre National pour la Recherche Scientifique et Technique (CNRST)
2011
Université Côte d'Azur
2011
Laboratoire des Interactions Plantes Micro-Organismes
2004-2005
Centre National de la Recherche Scientifique
2004
Institut de Pharmacologie Moléculaire et Cellulaire
2004
Significance Plant cells are surrounded by an extracellular matrix known as the cell wall. We have analyzed contribution of Arabidopsis wall to disease resistance pathogens with different parasitic styles. Here, we demonstrate that plant walls determinants immune responses since modification their composition in a set mutants has impact on and fitness phenotypes. In these genotypes, identified specific correlations between amounts carbohydrate epitopes resistance/fitness phenotypes through...
SummaryThe first detection of Aethina tumida Murray (the small hive beetle) in Italy occurred on 5 September 2014. Three nuclei containing honey bees (Apis mellifera) and located a clementine (citrus) orchard near an important international harbour the Calabria region (southern Italy) were heavily infested with adult larval A. tumida. infestation is notifiable disease European Union as well OIE listed disease. To prevent any introduction, importation regulated strictly (Commission Regulation...
Acute bee paralysis virus (ABPV), Black queen cell (BQCV), Chronic (CBPV), Deformed wing (DWV), Sacbrood (SBV) and Varroa destructor 1 (VDV1) are the six main honeybee viruses reported in Europe. We assessed accuracy (trueness precision) of reverse transcriptase quantitative TaqMan® PCR methods (RT-qPCR) for quantifying ABPV, BQCV, DWV, VDV1 SBV loads. Once systematic bias results had been corrected (overestimation ABPV BQCV quantification underestimation that VDV1), measurements were taken...
The class 1 pathogenesis-related (PR) proteins are thought to be involved in plant defence responses, but their molecular functions unknown. function of PR-1 was investigated tobacco by generating stable PR-1a-silenced lines which other acidic genes (PR-1b and PR-1c) were silenced. Plants lacking extracellular PR-1s more susceptible than wild-type plants the oomycete Phytophthora parasitica displayed unaffected systemic acquired resistance developmental this pathogen. Treatment with...
Besides the systemic acquired resistance (SAR) induced in response to microbial stimulation, host plants may also acquire pathogens endogenous stimuli associated with their own development. In tobacco (Nicotiana tabacum), vegetative-to-flowering transition comes along a susceptibility-to-resistance causal agent of black shank disease, oomycete Phytophthora parasitica. This affects infection effectiveness and hyphal expansion is extracellular accumulation cytotoxic activity that provokes...
Abstract Infectious and parasitic agents (IPAs) their associated diseases are major environmental stressors that jeopardize bee health, both alone in interaction with other stressors. Their impact on pollinator communities can be assessed by studying multiple sentinel species. Here, we analysed the field exposure of three managed species ( Apis mellifera , Bombus terrestris Osmia bicornis ) to 11 IPAs (six RNA viruses, two bacteria, microsporidia). The bees were deployed at 128 sites eight...
To provide a complete portrayal of the multiple factors negatively impacting insects in agricultural landscapes it is necessary to assess concurrent incidence, magnitude, and interactions among stressors over substantial biogeographical scales. Trans-national ecological field investigations with wide-ranging stakeholders typically encounter numerous challenges during design planning stages, not least that scientific soundness spatially replicated study must account for geographic climatic...
The activation of programmed cell death in the host during plant-pathogen interactions is an important component plant disease resistance mechanism. In this study we show that microorganisms also regulates interactions. We found a form vacuolar induced oomycete Phytophthora parasitica--the agent causes black shank Nicotiana tabacum--by extracellular stimuli from resistant tobacco. single-celled zoospores underwent characterized by dynamic membrane rearrangements, shrinkage, formation...
Among stressors affecting bee health, Nosema microsporidia are prevalent intracellular parasites. apis and ceranae have been described in honey bees (Apis spp.), while bombi has bumble (Bombus spp.). Although available molecular methods serve as a complement to microscopic diagnosis of nosemosis, they do not enable accurate quantification these three species. We developed quantitative real-time PCRs (qPCRs) starting from silico design specific primers, probes, recombinant plasmids, target...
Honey-bee colony losses are an increasing problem in Western countries. There many different causes, including infections due to various pathogens. Molecular biology techniques have been developed reliably detect and identify honey-bee The most sensitive, specific reliable is the quantitative real-time polymerase chain reaction (qPCR) methodology. This review of literature describes studies where qPCR was used detect, quantify four major pathogens: bacteria Paenibacillus larvae Melissococcus...
European beekeeping suffers significant regional differences in colony losses due to the external impacts on beekeeping, including climate change and prevalence of diseases (McMenamin Gener...
The Chronic bee paralysis virus (CBPV) is the aetiological agent of chronic paralysis, a contagious disease associated with nervous disorders in adult honeybees leading to massive mortalities front hives. Some clinical signs frequently reported, such as trembling, may be confused intoxication syndromes. Therefore, laboratory diagnosis using real-time PCR quantify CBPV loads used confirm disease. Clinical are usually viral higher than 108 copies genome per (8 log10 CBPV/bee). This threshold...
Pollinator insects play a crucial role in maintaining biodiversity and agricultural production worldwide. Yet they are subject to various infectious parasitic agents (IPAs). To better assess their exposure IPAs, discriminative quantitative molecular methods have been developed. These tools produce large datasets that need be summarised so as interpreted. In this paper, we described the calculation of three types composite indices (numerical, ordinal, nominal) characterize honey bee IPAs 128...
Summary Paenibacillus larvae is the causative agent of fatal American foulbrood disease in honeybees ( Apis mellifera ). Strain identification vital for preventing spread disease. To date, most accessible and robust scheme to identify strains multilocus sequence typing (MLST) method. However, this approach has limited resolution, especially epidemiological studies. As cost whole‐genome sequencing decreased as it becomes increasingly available laboratories, an extended MLST based on core...
Abstract Plant cell walls are complex structures subject to dynamic remodeling in response developmental and environmental cues, play essential functions disease resistance responses. We tested the specific contribution of plant immunity by determining susceptibility a set Arabidopsis wall mutants ( cwm ) pathogens with different parasitic styles: vascular bacterium, necrotrophic fungus biotrophic oomycete. Remarkably, most (31/38; 81.6%) showed alterations their responses at least one these...