A5050821686- Nematode management and characterization studies
- Insect Resistance and Genetics
- Legume Nitrogen Fixing Symbiosis
- Insect symbiosis and bacterial influences
- Insect-Plant Interactions and Control
- Plant Pathogens and Resistance
- Invertebrate Immune Response Mechanisms
- Plant-Microbe Interactions and Immunity
- Environmental DNA in Biodiversity Studies
- Genetic diversity and population structure
- Insect and Pesticide Research
- Carbohydrate Chemistry and Synthesis
- Enzyme Production and Characterization
- Genomics and Phylogenetic Studies
- Identification and Quantification in Food
- Plant Pathogenic Bacteria Studies
- Marine Sponges and Natural Products
- Plant tissue culture and regeneration
- Aquaculture disease management and microbiota
- Wildlife Ecology and Conservation
- Studies on Chitinases and Chitosanases
- Insect behavior and control techniques
- Model-Driven Software Engineering Techniques
- Parasite Biology and Host Interactions
- Plant Disease Resistance and Genetics
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2013-2024
Institut Sophia Agrobiotech
2006-2021
Centre National de la Recherche Scientifique
2007-2019
Université Côte d'Azur
2009-2019
Observatoire de la Côte d’Azur
2019
Institut National de la Recherche Agronomique
2001-2014
Zero to Three
2012
Fondation Sophia Antipolis
2009
Architecture et Fonction des Macromolécules Biologiques
2002-2003
Ecophysiologie et Génomique Fonctionnelle de la Vigne
2001
Plant-parasitic nematodes are major agricultural pests worldwide and novel approaches to control them sorely needed. We report the draft genome sequence of root-knot nematode Meloidogyne incognita, a biotrophic parasite many crops, including tomato, cotton coffee. Most assembled this asexually reproducing nematode, totaling 86 Mb, exists in pairs homologous but divergent segments. This suggests that ancient allelic regions M. incognita evolving toward effective haploidy, permitting new...
• Root-knot nematodes of the genus Meloidogyne are obligate biotrophic parasites able to infest > 2000 plant species. The nematode effectors responsible for disease development involved in adaptation parasite its host environment and response modulation. Here, differences between transcriptomes preparasitic exophytic second-stage juveniles (J2) parasitic endophytic third-stage (J3) incognita were investigated. Genes up-regulated at stage isolated by suppression subtractive hybridization...
• Root-knot nematodes (RKNs) are obligate endoparasites that maintain a biotrophic relationship with their hosts over period of several weeks and induce the differentiation root cells into specialized feeding cells. Nematode effectors synthesized in oesophageal glands injected plant tissue through syringe-like stylet certainly play central role these processes. In search for nematode effectors, we used comparative genomics on expressed sequence tag (EST) datasets to identify Meloidogyne...
Endoparasitoid wasps are important natural enemies of the widely distributed aphid pests and mainly used as biological control agents. However, despite increased interest on interaction networks, only sparse information is available factors by parasitoids to modulate physiology. Our aim was here identify major protein components venom injected at oviposition Aphidius ervi ensure successful development in its host, Acyrthosiphon pisum. A combined large-scale transcriptomic proteomic approach...
Microsatellites are the most popular source of molecular markers for studying population genetic variation in eukaryotes. However, few data currently available about their genomic distribution and abundance across phylum Nematoda. The recent completion genomes several nematode species, including Meloidogyne incognita, a major agricultural pest worldwide, now opens way comparative survey analysis microsatellites these organisms. Using MsatFinder, total numbers 1-6 bp perfect detected complete...
The principal architects of coral reefs are the scleractinian corals; these species divided in two major clades referred to as "robust" and "complex" corals. Although molecular diversity clade has received considerable attention, with several expressed sequence tag (EST) libraries a complete genome having been constructed, corals have far less despite fact that robust prominent focal points for ecological physiological studies. Filling this gap affords important opportunities extend studies...
Our present understanding of the functioning and evolutionary history invertebrate innate immunity derives mostly from studies on a few model species belonging to ecdysozoa. In particular, characterization signaling pathways dedicated specific responses towards fungi Gram-positive or Gram-negative bacteria in Drosophila melanogaster challenged our original view non-specific invertebrates. However, much remains be elucidated lophotrochozoan species. To investigate global specificity immune...
Abstract Background Genomic approaches provide unique opportunities to study interactions of insects with their pathogens. We developed a cDNA microarray analyze the gene transcription profile lepidopteran pest Spodoptera frugiperda in response injection polydnavirus HdIV associated ichneumonid wasp Hyposoter didymator . Polydnaviruses are parasitic ichneumonoid wasps and required for development within host, which they act as potent immunosuppressive In this study, we analyzed...
Coral reef ecosystems are renowned for their diversity and beauty. Their immense ecological success is due to a symbiotic association between cnidarian hosts unicellular dinoflagellate algae, known as zooxanthellae. These algae photosynthetic the cnidarian-zooxanthellae based on nutritional exchanges. Maintenance of such an intimate cellular partnership involves many crosstalks partners. To better characterize relationships host its symbionts, we conducted large-scale EST study sea anemone,...
Oomycetes are a group of filamentous microorganisms that includes both animal and plant pathogens causes major agricultural losses. Phytophthora species can infect most crops plants from natural ecosystems. Despite their tremendous economic ecologic importance, few effective methods exist for limiting the damage caused by these species. New solutions required, development will require improvements in our understanding molecular events governing infection pathogens. In this study, we...
Abstract Venom composition of parasitoid wasps attracts increasing interest – notably molecules ensuring parasitism success on arthropod pests but its variation within and among taxa is not yet understood. We have identified here the main venom proteins two braconid wasps, Psyttalia lounsburyi (two strains from South Africa Kenya) P. concolor, olive fruit fly parasitoids that differ in host range. Among shared abundant proteins, we found a GH1 β-glucosidase family leucine-rich repeat (LRR)...
• Data on plant pathogenic oomycetes are scarce and little is known about the early events leading to onset of infection. The aim this work was analyze penetration process soil-borne pathogen Phytophthora parasitica, which has a wide host range. Here, we performed cytological analysis colonization first cell developed an inoculation assay for characterizing entire through cellular molecular analyses. We showed that P. parasitica infects roots by producing specialized structure, appressorium....
We report here for the first time isolation and characterization of a protein from organic matrix (OM) sclerites alcyonarian, Corallium rubrum. This named scleritin is one predominant proteins extracted EDTA-soluble fraction OM. The entire open reading frame (ORF) was obtained by comparing amino acid sequences de novo mass spectrometry Edman degradation with an expressed sequence tag library dataset C. Scleritin secreted basic phosphorylated which exhibits short 135 acids signal peptide 20...
The widespread use of genome sequencing provided evidences for the high degree conservation in innate immunity signalling pathways across animal phyla. However, functioning and evolutionary history immune-related genes remains unknown most invertebrate species. A striking observation coming from analysis pea aphid Acyrthosiphon pisum is absence important conserved known to be involved antimicrobial responses other insects. This reduction antibacterial immune defences thought related their...
Data management has emerged as one of the central issues in high-throughput processes taking a protein target sequence through to sample. To simplify this task, and following extensive consultation with international structural genomics community, we describe here model data related production. The is suitable for both large small facilities use tracking samples, experiments, results many procedures involved. described Unified Modeling Language (UML). In addition, present relational database...
Many invasive species undergo a significant reduction in genetic diversity, i.e. bottleneck, the early stages of invasion. However, this does not necessarily prevent them from achieving considerable ecological success and becoming highly efficient colonizers. Here we tested so-called purge hypothesis, which suggests that demographic bottlenecks may indeed help to deleterious mutations, thereby reducing load. We used transcriptome-based exome capture protocol identify thousands SNPs coding...