A5021286307- Metabolomics and Mass Spectrometry Studies
- Radiomics and Machine Learning in Medical Imaging
- Thyroid Cancer Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Acute Ischemic Stroke Management
- Genetics, Aging, and Longevity in Model Organisms
- Radiopharmaceutical Chemistry and Applications
- Tryptophan and brain disorders
- Mass Spectrometry Techniques and Applications
- Telomeres, Telomerase, and Senescence
- Ferroptosis and cancer prognosis
- Cell death mechanisms and regulation
- Advanced Proteomics Techniques and Applications
- Enzyme Structure and Function
- Plant Physiology and Cultivation Studies
- Cancer Research and Treatments
- Cancer, Lipids, and Metabolism
- Plant Reproductive Biology
- Medical Imaging Techniques and Applications
- Trace Elements in Health
- Analytical Chemistry and Chromatography
- Diet and metabolism studies
- Plant tissue culture and regeneration
- Cancer-related Molecular Pathways
- Marine Sponges and Natural Products
Université Côte d'Azur
2014-2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2014-2024
Direction de la Recherche Fondamentale
2020-2024
Frédéric Joliot Institute for Life Sciences
2020-2024
Institut de Biosciences et Biotechnologies
2019-2021
Unité de recherche sur les maladies cardiovasculaires et métaboliques
2021
Institut de Biosciences et biotechnologies d'Aix-Marseille
2019-2020
Institut de Biologie Valrose
2018
Centre Antoine Lacassagne
2012
Inserm
2007
Telomere shortening is a hallmark of aging and counteracted by telomerase. As in humans, the zebrafish gut one organs with fastest rate telomere decline, triggering early tissue dysfunction during normal prematurely aged telomerase mutants. However, whether telomere-dependent an individual organ, gut, causes systemic unknown. Here we show that tissue-specific expression can prevent rescues premature tert-/-. Induction senescence low cell proliferation, while restoring integrity, inflammation...
The dipeptide N-acetylglutaminylglutamine amide (NAGGN) was discovered in the bacterium Sinorhizobium meliloti grown at high osmolarity, and subsequently shown to be synthesized accumulated by a few osmotically challenged bacteria. However, its biosynthetic pathway remained unknown. Recently, two genes, which putatively encode glutamine amidotransferase an acetyltransferase are up-regulated osmotic stress, were identified Pseudomonas aeruginosa . In this work, locus carrying orthologous...
Retinoic acid synthesis is not essential for meiosis in fetal ovaries, challenging the dogma about meiosis-inducing factors.
The full chemical reinvestigation of the Mediterranean marine sponge Crambe crambe led to isolation and structural characterization 11 crambescin derivatives, including 8 new compounds, together with known crambescidin 816. HRMS/MS studies allowed complete assignment alkyl chain lengths these guanidine alkaloids while absolute configurations all compounds were inferred from comparison between experimental theoretical circular dichroism spectra. Crambescidin 816 was proven be more cytotoxic...
Genomics and transcriptomics have led to the widely-used molecular classification of breast cancer (BC). However, heterogeneous biological behaviors persist within subtypes. Metabolomics is a rapidly-expanding field study dedicated cellular metabolisms affected by environment. The aim this was compare metabolomic signatures BC obtained 5 different unsupervised machine learning (ML) methods. Fifty-two consecutive patients with an indication for adjuvant chemotherapy between 2013 2016 were...
The role of ketone bodies in the cerebral energy homeostasis neurological diseases has begun to attract recent attention particularly acute diseases. In ketogenic therapies, ketosis is achieved by either a diet or administration exogenous bodies. oral ingestion ester (KE), (R)-3-hydroxybutyl (R)-3-hydroxybutyrate, new method generate rapid and significant (i.e., above 6 mmol/L) humans. KE hydrolyzed into β-hydroxybutyrate (βHB) its precursor 1,3-butanediol. Here, we investigate effect (3 mg...
Formation and stabilization of postsynaptic glycine receptor (GlyR) clusters result from their association with the polymerized scaffold protein gephyrin. At cell surface, lateral diffusion local trapping GlyR by synaptic gephyrin is one main factors controlling number. However, mechanisms regulating gephyrin/GlyR cluster sizes are not fully understood. To identify molecular binding partners able to control stability, we performed pull-down assays full-length or truncated forms incubated in...
Cancer stem cells (CSCs) represent a minor population of self-renewing cancer that fuel tumor growth. As CSCs are generally spared by conventional treatments, this is likely to be responsible for relapses observed in most cancers. In work, we analyzed the preventive efficiency CSC-based vaccine on development liver metastasis from colon syngeneic rat model. We isolated CSC-enriched PROb carcinoma cell line basis expression aldehyde dehydrogenase-1 (ALDH1) marker. Comparative analysis...
Abstract Renal cell carcinomas (RCC) are classified according to their histological features. Accurate classification of RCC and comprehensive understanding metabolic dysregulation critical importance. Here we investigate the use metabolomic analyses classify main subtypes describe variation for each subtype. To this end, performed profiling 65 frozen samples (40 clear cell, 14 papillary 11 chromophobe) using liquid chromatography-mass spectrometry. OPLS-DA multivariate analysis based on...
In vitro cultures showed that the proximal buds isolated from a rose (Rosa hybrida L. cv. Ruidriko Vivaldi®) stem were endodormant. Growth and high percentage of bud break could be observed when treated with fluridone, an inhibitor carotenoid synthesis. Flow cytometry determination nuclear DNA content revealed cell cycle activity endodormant was arrested in G 1 phase. Upon culture, large decrease ABA responsible for progress G1 to G2 phase whatever culture medium. However, control neither...
Unmethylated CpG dinucleotides, present in bacterial DNA, are recognized vertebrates via the Toll-like receptor 9 (TLR9) and known to act as an anticancer agent by stimulating immune cells induce a proinflammatory response. Although effects of CpG-oligodeoxynucleotides (CpG-ODNs) have been widely studied, little is regarding their molecular TLR9-positive tumor cells. To better understand role these motifs cancer cells, we analyzed proteome modifications induced vitro vivo after CpG-ODN...
We report here for the first time isolation and characterization of a protein from organic matrix (OM) sclerites alcyonarian, Corallium rubrum. This named scleritin is one predominant proteins extracted EDTA-soluble fraction OM. The entire open reading frame (ORF) was obtained by comparing amino acid sequences de novo mass spectrometry Edman degradation with an expressed sequence tag library dataset C. Scleritin secreted basic phosphorylated which exhibits short 135 acids signal peptide 20...
The diagnosis of cardioembolic stroke can be challenging for patient management in secondary prevention, particularly the case covert paroxysmal atrial fibrillation. molecular composition a cerebral thrombus is related to its origin. Therefore, proteomic and metabolomic analyses retrieved thrombotic material should allow identification biomarkers or signatures improve etiological stroke.In this pilot study, proteome metabolome thrombi from atherothrombotic patients were studied according...
The bacterial cytosine deaminase (CD) gene, associated with the 5-fluorocytosine (5FC) prodrug, is one of most widely used suicide systems in gene therapy. Introduction CD within a tumor induces, after 5FC treatment animal, local production 5-fluorouracil resulting intratumor chemotherapy. Destruction gene-modified then followed by triggering an antitumor immune reaction regression distant wild-type metastasis. global effects on colorectal adenocarcinoma cells expressing were analyzed using...
Abstract We studied Tcirg1 gene expression on RANKL-induced osteoclastic differentiation of the mouse model RAW264.7 cells. identified a mechanism involving PARP-1 inhibition release and JunD/Fra-2 binding, which is responsible for upregulation. Introduction: The encodes a3 isoform V-ATPase subunit, plays critical role in resorption activity osteoclast. Using serial deletion constructs promoter, we performed transcriptional study to identify factor(s) involved regulation expression....
Perturbation of thyroid iodide uptake is a well-documented side effect the use iodinated contrast media (ICM) administered intravenously. This thought to be mediated by free in ICM formulations, but this hypothesis has never been formally proven. The aim present study was assess validity hypothesis. <b>Methods:</b> We used mass spectrometry analysis quantify free-iodide contamination ICM. Established cell lines expressing Na/I symporter (NIS) were on uptake. SPECT/CT measure vivo...
The COVID-19 pandemic has been a serious worldwide public health crisis since 2020 and is still challenging healthcare systems. New tools for the prognosis diagnosis of patients remain important issues.Here, we studied metabolome plasma samples identification biomarkers.Plasma eighty-six SARS-CoV-2-infected subjects 24 healthy controls were collected during first peak in France 2020.Plasma fingerprinting allowed successful discrimination controls, mild SARS-CoV-2 subjects, moderate severe at...
For decades, sodium/iodide symporter NIS-mediated iodide uptake has played a crucial role in the radioactive ablation of thyroid cancer cells. NIS-based gene therapy also become promising tool for treatment tumors extrathyroidal origin. But its applicability been hampered by reduced expression NIS, resulting moderated capacity to accumulate
The Tcirg1 gene encodes the osteoclast-specific a3 isoform of V-ATPase a subunit. Using mouse osteoclastic model RAW264.7 cells, we studied expression, and identified PARP-1 as transcriptional repressor negatively regulated by RANKL during osteoclastogenesis.The TCIRG1 subunit, mutations at this locus account for approximately 60% infantile malignant osteopetrosis cases. cells an differentiation model, undertook study focused on 4-kb region upstream transcription starting point.The promoter...
The dramatic clinical improvement offered by mechanical thrombectomy raised questions about the relevance of prior intravenous thrombolysis in large-vessel occlusion strokes. Hence, studying susceptibility and its dependence on thrombus composition is crucial. We used an observational proteomic study whole thrombi retrieved to identify factors associated with fibrin content fibrinolytic activity (FA). In 104 stroke patients, proteome was established mass spectrometry coupled liquid...
To better understand the effects of antiandrogens on prostate, we investigated changes in proteome rat ventral prostate (VP) following treatment with a well characterized 5α-reductase inhibitor, finasteride. Sprague-Dawley rats were treated daily by gavage finasteride at 0, 1, 5, 25, and 125 mg/kg/day. Changes plasma hormone levels as weight histology sex accessory tissues determined after 28 days showed dose-related decrease VP weights together marked atrophy tissue visible macroscopic...