A5087392969- Mitochondrial Function and Pathology
- Metabolism and Genetic Disorders
- Immune cells in cancer
- Barrier Structure and Function Studies
- Traumatic Brain Injury and Neurovascular Disturbances
- Peroxisome Proliferator-Activated Receptors
- Autism Spectrum Disorder Research
- Plant Molecular Biology Research
- Adipose Tissue and Metabolism
- Neurological diseases and metabolism
- Genetics and Neurodevelopmental Disorders
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Medicinal Plants and Bioactive Compounds
- Congenital heart defects research
- Amino Acid Enzymes and Metabolism
- Tryptophan and brain disorders
- Metabolomics and Mass Spectrometry Studies
- Immune Response and Inflammation
- Diet and metabolism studies
- Lipid metabolism and biosynthesis
- Neurological Disease Mechanisms and Treatments
University of Ottawa
2023-2024
Ottawa Hospital
2023
Ottawa Hospital Research Institute
2023
Direction de la Recherche Fondamentale
2021
Frédéric Joliot Institute for Life Sciences
2021
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2021
The role of ketone bodies in the cerebral energy homeostasis neurological diseases has begun to attract recent attention particularly acute diseases. In ketogenic therapies, ketosis is achieved by either a diet or administration exogenous bodies. oral ingestion ester (KE), (R)-3-hydroxybutyl (R)-3-hydroxybutyrate, new method generate rapid and significant (i.e., above 6 mmol/L) humans. KE hydrolyzed into β-hydroxybutyrate (βHB) its precursor 1,3-butanediol. Here, we investigate effect (3 mg...
<title>Abstract</title> Major depressive disorder (MDD) is a leading cause of disease burden worldwide with low therapeutic efficacy. The blood-brain barrier (BBB) essential to maintain brain homeostasis and regulate drug delivery. Chronic social stress, MDD’s main environmental risk factor, associated neurovascular pathology in mice including loss BBB integrity mood-regulating regions sex-specific manner, which also observed individuals MDD. Pericytes ensheathe endothelial cells modulate...
Neurovascular abnormalities in mouse models of 16p11.2 deletion autism syndrome are reminiscent alterations reported murine glucose transporter deficiency, including reduced brain angiogenesis and behavioral alterations. Yet, whether cerebrovascular 16p11.2df/+ mice affect metabolism is unknown. Here, we report that anesthetized display elevated uptake, a phenomenon recapitulated with endothelial-specific haplodeficiency. Awake attenuated relative fluctuations extracellular following...
Type 2 cytokines like IL-4 are hallmarks of helminth infection and activate macrophages to limit immunopathology mediate clearance. In addition cytokines, nutrients metabolites critically influence macrophage polarization. Choline is an essential nutrient known support normal responses lipopolysaccharide; however, its function in polarized by type unknown. Using murine IL-4-polarized macrophages, targeted lipidomics revealed significantly elevated levels phosphatidylcholine, with select...
ABSTRACT Lipids are essential for neuron development and physiology. Yet, the central hubs that coordinate lipid supply demand in neurons remain unclear. Here, we combine invertebrate vertebrate models to establish presence functional significance of neuronal droplets (LD) vivo . We find LD normally present a non-uniform distribution across brain, demonstrate triglyceride metabolism enzymes droplet-associated proteins control formation through both canonical recently-discovered pathways....
Abstract Transmission electron microscopy (TEM) is a powerful imaging technique, yielding ultrastructural investigation of organic and non-organic samples. Despite its ability to reach nanoscale resolutions, conventional TEM presents major disadvantage by only acquiring two-dimensional snapshots, thus hindering our volumetric understanding Electron tomography (ET) overcomes this limitation offering detailed views thin specimen in 3 dimensions (3D). This technique widely used biology has...
Abstract Type 2 cytokines like IL-4 are hallmarks of helminth infection and activate macrophages to limit immunopathology mediate clearance. In addition cytokines, nutrients metabolites critically influence macrophage polarization. Choline is an essential nutrient known support normal responses lipopolysaccharide; however, its function in polarized by type unknown. Using murine IL-4-polarized macrophages, targeted lipidomics revealed significantly elevated levels phosphatidylcholine, with...