A5016108356- DNA and Nucleic Acid Chemistry
- Photochemistry and Electron Transfer Studies
- Luminescence and Fluorescent Materials
- Advanced biosensing and bioanalysis techniques
- Advanced Fluorescence Microscopy Techniques
- Molecular Sensors and Ion Detection
- Adenosine and Purinergic Signaling
- Photochromic and Fluorescence Chemistry
- Click Chemistry and Applications
- Photoreceptor and optogenetics research
- Organic Light-Emitting Diodes Research
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
Chalmers University of Technology
2024
AstraZeneca (Sweden)
2023-2024
Centre National de la Recherche Scientifique
2019-2023
Institut de Chimie de Nice
2019-2023
Université Côte d'Azur
2019-2023
Here, we present the non-linear spectroscopic characterization of fluorescent nucleobase analogue 2CNqA as a monomer and inside an antisense oligonucleotide. Its applicability 2P reporter is demonstrated using live cell imaging.
Abstract Fluorescent base analogues (FBAs) have become useful tools for applications in biophysical chemistry, chemical biology, live‐cell imaging, and RNA therapeutics. Herein, two synthetic routes towards a novel FBA of uracil named qU (quadracyclic uracil/uridine) are described. The nucleobase bears tetracyclic fused ring system is designed to allow specific Watson‐Crick pairing with adenine. We find that absorbs light the visible region spectrum emits brightly quantum yield 27 %...
Abstract In this work, we report on the Photoinduced Electron Transfer (PET) reaction between a donor (adenine analogue) and an acceptor (3‐methoxychromone dye, 3MC ) in context of designing efficient fluorescent probes as DNA sensors. Firstly, Gibbs energy was investigated disconnected donor–acceptor systems by Rehm‐Weller equation. The oxidation potential adenine derivative responsible for exergonicity PET separated combinations. Then, donor‐π‐acceptor conjugates using steady‐state...
The intensive research for hybridization probes based on organic molecules with fluorogenic properties is currently attracting particular attention due to their potential efficiently recognize different DNA conformations and the local environment. However, most established chromophores do not meet requirements of this task, as they exhibit good brightness in aqueous buffer media, develop aggregation and/or are easily conjugated oligodeoxynucleotides (ODNs) while keeping photophysics intact....
Abstract RNA and its building blocks play central roles in biology have become increasingly important as therapeutic agents targets. Hence, probing understanding their dynamics cells is important. Fluorescence microscopy offers live-cell spatiotemporal monitoring but requires labels. We present two fluorescent adenine analogue nucleoside phosphates which show spontaneous uptake accumulation cultured human cells, likely via transporters, potential utilization cellular Upon uptake, one...
Forced intercalation (FIT) probes have proven to be a reliable, rapid, inexpensive, and accurate method for the detection visualization of specific nucleic acid sequences. The accommodation rationally designed chromone-based fluorogen within double-stranded DNA structure was investigated by UV–Vis spectrophotometry steady-state fluorescence spectroscopy under physiological pH conditions. After selective excitation matching with 350 nm laser, intrinsically negligible tethered electroneutral...