A5077302854- Autophagy in Disease and Therapy
- MicroRNA in disease regulation
- Bone Metabolism and Diseases
- Virus-based gene therapy research
- Bone and Joint Diseases
- RNA Interference and Gene Delivery
- Cancer Research and Treatments
- Radioactive element chemistry and processing
- Immunotherapy and Immune Responses
- Bone health and treatments
- Orthopaedic implants and arthroplasty
- Radioactivity and Radon Measurements
- Extracellular vesicles in disease
- Cancer Cells and Metastasis
- Epigenetics and DNA Methylation
- Trace Elements in Health
- Advanced X-ray and CT Imaging
- Mesenchymal stem cell research
- Cancer, Hypoxia, and Metabolism
- Radiation Dose and Imaging
- Animal Virus Infections Studies
- Bone health and osteoporosis research
- Immune Cell Function and Interaction
- Plant Virus Research Studies
- Colorectal Cancer Treatments and Studies
Université Côte d'Azur
2014-2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2016-2024
Inserm
1996-2024
Frédéric Joliot Institute for Life Sciences
2021-2024
Institut de Chimie de Nice
2021
Institut de Biosciences et Biotechnologies
2016-2021
Centre National de la Recherche Scientifique
2010-2012
Institut National de Recherche en Santé Publique
2007
Centre Antoine Lacassagne
1998-1999
Hôpital Pasteur
1999
Bone remodeling is a tightly controlled mechanism in which osteoblasts (OB), the cells responsible for bone formation, osteoclasts (OC), specialized resorption, and osteocytes, multifunctional mechanosensing embedded matrix, are main actors. Increased oxidative stress OB, producing mineralizing has been associated with osteoporosis development but role of autophagy OB not yet addressed. This goal present study. We first show that autophagic process induced during mineralization. Then, using...
Dihydropyrimidine dehydrogenase (DPD) is the initial and rate-limiting enzyme of 5-fluorouracil (5-FU) catabolism. We report lymphocytic DPD data concerning a group 53 patients (23 men, 30 women, mean age 58, range 36-73), treated by 5-FU-based chemotherapy in different French institutions who developed unanticipated 5-FU-related toxicity. Lymphocyte samples (standard collection procedure) were sent to us for determination (biochemical method). Among whole patients, 19 had significant...
Age-related bone loss is associated with an increased oxidative stress which worsened by estrogen fall during menauposis. This observation has drawn attention to autophagy, a major cellular catabolic process, able alleviate in osteoblasts (OB) and osteocytes (OST), two key cell types. Moreover, autophagy decline can be aging, suggesting that age-related deficiency OB and/or OST could contribute skeletal aging osteoporosis onset.In the present work, activity was analyzed male female mice...
Cancer stem cells (CSCs) represent a minor population of self-renewing cancer that fuel tumor growth. As CSCs are generally spared by conventional treatments, this is likely to be responsible for relapses observed in most cancers. In work, we analyzed the preventive efficiency CSC-based vaccine on development liver metastasis from colon syngeneic rat model. We isolated CSC-enriched PROb carcinoma cell line basis expression aldehyde dehydrogenase-1 (ALDH1) marker. Comparative analysis...
BACKGROUND: The cytosine deaminase gene of Escherichia coli converts the nontoxic compound 5-fluorocytosine into 5-fluorouracil (5-FU), thereby acting as a suicide when introduced cancer cells, killing cells they are exposed to 5-fluorocytosine. We analyzed efficacy using deaminase-bearing an autologous tumor vaccine in rat model that mimics liver metastasis from colon carcinoma. METHODS: plasmid vector containing E. BDIX carcinoma cell line. Intrahepatic injection modified syngeneic animals...
Unmethylated CpG dinucleotides, present in bacterial DNA, are recognized vertebrates via the Toll-like receptor 9 (TLR9) and known to act as an anticancer agent by stimulating immune cells induce a proinflammatory response. Although effects of CpG-oligodeoxynucleotides (CpG-ODNs) have been widely studied, little is regarding their molecular TLR9-positive tumor cells. To better understand role these motifs cancer cells, we analyzed proteome modifications induced vitro vivo after CpG-ODN...
Cancer stem cells (CSCs) represent a minor population of cancer with cell-like properties which are able to fuel tumor growth and resist conventional treatments. Autophagy has been described be upregulated in some CSCs play crucial role by maintaining features promoting resistance both hostile microenvironments Osteosarcoma (OS) is an aggressive bone mainly affects children adolescents autophagy OS poorly studied. However, this very interesting case because often deregulated cancer. In the...
Osteoporosis (OP) is a pathology characterized by bone fragility affecting 30% of postmenopausal women, mainly due to estrogen deprivation and increased oxidative stress. An autophagy involvement suspected in OP pathogenesis but definitive proof humans remains be obtained.
The bacterial cytosine deaminase (CD) gene, associated with the 5-fluorocytosine (5FC) prodrug, is one of most widely used suicide systems in gene therapy. Introduction CD within a tumor induces, after 5FC treatment animal, local production 5-fluorouracil resulting intratumor chemotherapy. Destruction gene-modified then followed by triggering an antitumor immune reaction regression distant wild-type metastasis. global effects on colorectal adenocarcinoma cells expressing were analyzed using...
AbstractIn addition to its clean-up function, autophagy is considered as an innate immunity mechanism due role in the removal of intracellular pathogens. Toll-like receptors (TLRs) are crucial components involved recognition a diverse array microbial products. Recent works demonstrated that different pathogen-associated molecular patterns (PAMPs) such lipopolysaccharide (LPS) and single-strand RNA able induce via TLRs immune cells. In recent report, we showed bacterial CpG motifs, another...
The cytosine deaminase (CD) gene converts the nontoxic prodrug, 5-fluorocytosine (5-FC), into 5-fluorouracil (5-FU). We previously showed that injection of CD-bearing cancer cells followed by 5-FC treatment can act as an autologous tumor vaccine in a syngenic liver metastasis model rats. In present work, we analyzed antitumor efficiency direct intratumoral CD-expressing plasmid. rats bearing microscopic or macroscopic metastases right and left lobes, plasmid was performed lobe tumor,...