A5031523396- Invertebrate Immune Response Mechanisms
- Antimicrobial Peptides and Activities
- Insect symbiosis and bacterial influences
- Insect Resistance and Genetics
- Neurobiology and Insect Physiology Research
- Mosquito-borne diseases and control
- Aquaculture disease management and microbiota
- Insect and Pesticide Research
- RNA and protein synthesis mechanisms
- Immune Response and Inflammation
- Transgenic Plants and Applications
- Silk-based biomaterials and applications
- RNA Interference and Gene Delivery
- Insect Utilization and Effects
- Biochemical and Structural Characterization
- interferon and immune responses
- Animal Genetics and Reproduction
- Ubiquitin and proteasome pathways
- Escherichia coli research studies
- Sperm and Testicular Function
- Protease and Inhibitor Mechanisms
- Parasites and Host Interactions
- Chemical Synthesis and Analysis
- NF-κB Signaling Pathways
- Toxin Mechanisms and Immunotoxins
Université de Strasbourg
2010-2021
Institut de Biologie Moléculaire et Cellulaire
2005-2021
Innate Pharma (France)
2020-2021
University of Puerto Rico at Carolina
2020-2021
Centre National de la Recherche Scientifique
2006-2020
Modèles Insectes de l'Immunité Innée
1992-2018
Institut de Biologie Moléculaire des Plantes
2000-2018
Centre de Génétique Moléculaire
2002
Exelixis (United States)
2001
European Molecular Biology Laboratory
1995
Insects respond to microbial infection by the rapid and transient expression of several genes encoding potent antimicrobial peptides. Herein we demonstrate that this response Drosophila is not aspecific but can discriminate between various classes microorganisms. We first observe antibacterial antifungal peptides are differentially expressed after injection distinct More strikingly, naturally infected entomopathogenic fungi exhibit an adapted producing only with activities. This mediated...
In this paper we report a recessive mutation, immune deficiency (imd), that impairs the inducibility of all genes encoding antibacterial peptides during response Drosophila. When challenged with bacteria, flies carrying mutation show lower survival rate than wild-type flies. We also that, in contrast to peptides, antifungal peptide drosomycin remains inducible homozygous imd mutant background. These results point existence two different pathways leading expression types target genes, either...
The antifungal defense of Drosophila is controlled by the spaetzle/Toll/cactus gene cassette. Here, a loss-of-function mutation in encoding blood serine protease inhibitor, Spn43Ac, was shown to lead constitutive expression peptide drosomycin, and this effect mediated spaetzle Toll products. Spaetzle cleaved proteolytic enzymes its active ligand form shortly after immune challenge, constitutively present Spn43Ac-deficient flies. Hence, Spn43Ac negatively regulates signaling pathway, does not...
Oligonucleotide DNA microarrays were used for a genome-wide analysis of immune-challenged Drosophila infected with Gram-positive or Gram-negative bacteria, fungi. Aside from the expression an established set immune defense genes, significant number previously unseen immune-induced genes found. Genes particular interest include corin - and Stubble -like both which have type II transmembrane domain; easter snake may fulfil roles in Toll pathway; masquerade gene, potentially involved enzyme...
Drosophila host defense to fungal and Gram-positive bacterial infection is mediated by the Spaetzle/Toll/cactus gene cassette. It has been proposed that Toll does not function as a pattern recognition receptor per se but activated through cleaved form of cytokine Spaetzle. The upstream events linking cleavage Spaetzle have long remained elusive. Here we report identification central component activation Toll. We show ethylmethane sulfonate–induced mutations in persephone gene, which encodes...
We have isolated from the hemolymph of immunized larvae dipteran insect Phormia terranovae two peptides that are selectively active against Gram-positive bacteria. They positively charged 40 residues containing three intramolecular disulfide bridges and differ one another by only a single amino acid. These neither functionally nor structurally related to any known immune response but show significant homology microbicidal cationic mammalian granulocytes (defensins). propose name "insect...
One of the characteristics host defense higher insects is rapid and transient synthesis a variety potent antimicrobial peptides. To date, several distinct inducible peptides or peptide families have been totally partially characterized. We present here isolation characterization novel 26-residue proline-rich immune-inducible from Drosophila, which exhibits both antibacterial (Gram-positive) antifungal activities. Peptide sequencing cDNA cloning indicate presense two isoforms in our...
One of the characteristics host defense insects is rapid synthesis a variety potent antibacterial and antifungal peptides. To date, seven types inducible antimicrobial peptides (AMPs) have been characterized in Drosophila . The importance these supported by observation that flies deficient for Toll or Immune deficiency (Imd) pathway, which affects AMP gene expression, are extremely susceptible to microbial infection. Here we developed genetic approach address functional relevance defined...
Injection of heat-killed bacteria into larvae the large tenebrionid beetle Zophobas atratus (Insecta, Endopterygota, Coleoptera) results in appearance hemolymph a potent antibacterial activity as evidenced by plate growth inhibition assay. We have isolated three peptides (A-C) from this immune which probably account for most activity. Their primary structures were established combination peptide sequencing and molecular mass determination spectrometry. Peptide A, is bactericidal against...
Insect defensins are a family of 4‐kDa, cationic, inducible antibacterial peptides which bear six cysteine residues engaged in three intramolecular disulfide bridges. They owe their name to certain sequence similarities with from mammalian neutrophiles and macrophages. We report the characterization novel defensin isoform Drosophila cloning gene encoding preprodefensin. The gene, is intronless present single copy/haploid genome, maps at position 46CD on right arm second chromosome. analysis...