- Nematode management and characterization studies
- Legume Nitrogen Fixing Symbiosis
- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- Plant Parasitism and Resistance
- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- Plant Genetic and Mutation Studies
- Insect Pest Control Strategies
- Plant Disease Resistance and Genetics
- Plant Disease Management Techniques
- Plant and Fungal Interactions Research
- Insect-Plant Interactions and Control
- Cassava research and cyanide
- Genetic and Environmental Crop Studies
- Plant tissue culture and regeneration
- Seed Germination and Physiology
- Metabolism and Genetic Disorders
- Renal and related cancers
- Mitochondrial Function and Pathology
- Soybean genetics and cultivation
- Plant Reproductive Biology
- Urological Disorders and Treatments
- Phytoplasmas and Hemiptera pathogens
- Plant responses to elevated CO2
Institute for Sustainable Plant Protection
2015-2024
National Research Council
2000-2024
Istituto Nazionale di Fisica Nucleare, Sezione di Bari
2006-2015
National Academies of Sciences, Engineering, and Medicine
2013
University of Pisa
2005
Istituto di Biologia e Biotecnologia Agraria
2000
University of Bari Aldo Moro
2000
German Cancer Research Center
1998
DKFZ-ZMBH Alliance
1998
Plant-parasitic nematodes are major agricultural pests worldwide and novel approaches to control them sorely needed. We report the draft genome sequence of root-knot nematode Meloidogyne incognita, a biotrophic parasite many crops, including tomato, cotton coffee. Most assembled this asexually reproducing nematode, totaling 86 Mb, exists in pairs homologous but divergent segments. This suggests that ancient allelic regions M. incognita evolving toward effective haploidy, permitting new...
Here, the interaction of Melodoigyne incognita virulent and avirulent pathotypes with susceptible Mi-resistant tomato (Solanum lycopersicon) has been studied. Significant differences in nematode penetration occurred 2 days postinoculation (dpi) became stable from 3 dpi onwards. The hypersensitive cell response (HR) resistant plants prevented installation pathotype. pathotype overcame Mi (nematode) resistance induced feeding sites root cells without triggering HR. Reactive oxygen species...
Summary An increase in the production of reactive oxygen species (ROS) is a typical event occurring during different stress conditions and activating conflicting responses plants. In order to investigate relevance timing amounts ROS production, tobacco ( Nicotiana tabacum ) Bright Yellow‐2 (TBY‐2) cells were incubated with glucose plus oxidase, for generating H 2 O time, or directly known . Data presented here indicate that, TBY‐2 cells, difference level critical point shifting metabolic...
The expression pattern of pathogenesis-related genes PR-1, PR-2 and PR-5, considered as markers for salicylic acid (SA)-dependent systemic acquired resistance (SAR), was examined in the roots shoots tomato plants pre-treated with SA subsequently infected root-knot nematodes (RKNs) (Meloidogyne incognita). PR-1 up-regulated both SA-treated plants, whereas PR-5 enhanced only roots. over-expression whole plant occurred soon 1 day after treatment. Up-regulation gene to be main marker SAR...
Beneficial microorganisms are generally known to activate plant defense against biotic challenges. However, the molecular mechanisms by which activated plants react more rapidly and actively pests remain still largely unclear. Tomato pre-treated with a mixture of beneficial bio-control agents (BCAs), as soil-drenches, were less sensitive infection root-knot nematode (RKN) Meloidogyne incognita. To unravel this induced resistance RKNs, we used qRT-PCR monitor expression, in tomato roots...
This work provides novel insights into the effects caused by histone deacetylase inhibitor trichostatin A (TSA) during Medicago truncatula seed germination, with emphasis on repair response. Seeds treated H2O and TSA (10 20 μM) were collected imbibition (8 h) at radicle protrusion phase. Biometric data showed delayed germination impaired seedling growth in TSA-treated samples. Comet assay, performed radicles phase 4-days old M. seedlings, revealed accumulation of DNA strand breaks upon...
Genetic resistance in plants against incompatible pests is expressed by the activation of an immune system; however, molecular mechanisms pest recognition and expression immunity, although long object investigation, are far from being fully understood. The response triggered infection soil-borne parasites, such as root-knot nematodes (RKNs), to resistant tomato was studied compared compatible that occurred when RKNs attacked susceptible plants. In interactions, invading nematode juveniles...
Summary Viral infections are accompanied by a massive production of small interfering RNAs (siRNAs) plant origin, such as virus‐activated (va)siRNAs, which drive the widespread silencing host gene expression, and whose effects in pathogen interactions remain unknown. By combining phenotyping molecular analyses, we characterized vasiRNAs that associated with typical mosaic symptoms cauliflower virus infection two crops, turnip ( Brassica rapa ) oilseed rape napus ), reference Arabidopsis...
Recently, studies on climate change have highlighted the central role of photosynthetic mechanisms in defense response plants to abiotic and biotic stresses. Photo-sensing photo-activation are innovative technologies applied for early detection plant pathogens order prevent dramatic impact they may plants. Chlorophyll Fluorescence Imaging (CFI) Laser-Induced Breakdown Spectroscopy (LIBS) analytical techniques can be used evaluate amount chlorophyll plants, which altered case In this work,...
Arbuscular mycorrhizal fungi (AMF) are generally recognized to induce plant growth and prime plants against soil-borne parasites, such as parasitic nematodes. However, the effectiveness of commercial formulates containing AMF has been questioned. Increasing amounts per one AMF-containing formulate, reported in text Myco, were used detect effects on tomato resistance induced root-knot nematodes (RKNs) The doses (0.5, 1.0, 2.0 g, Myco1, Myco2, Myco3, respectively) soil-drenched growing potted...
Two wild-type field populations of root-knot nematodes (Mi-Vfield, Mj-TunC2field), and two isolates selected for virulence in laboratory on resistant tomato cultivars (SM2V, SM11C2), were used to induce a resistance reaction the soil-borne parasites. Epigenetic metabolic mechanisms detected compared with those occurring partially or fully successful infections. The activated epigenetic plant resistance, as opposed infected plants, by analyzing methylated status total DNA, ELISA methods,...
A cDNA of 312 bp, similar to polygalacturonase-inhibiting proteins (PGIPs), was isolated by cDNA-amplified fragment length polymorphism (cDNA-AFLP) from pea roots infected with the cyst nematode Heterodera goettingiana. The deduced amino acid sequence obtained complete Pspgip1 coding very PGIPs described several other plant species, and identical in both MG103738 Progress 9 genotypes, resistant susceptible H. goettingiana, respectively. Reverse transcription-polymerase chain reaction...
Preneoplastic liver foci and neoplasms of different morphological phenotypes were induced in rats with N-nitrosomorpholine (NNM; 120 mg/l drinking water for 7 weeks) the peroxisome proliferator dehydroepiandrosterone (DHEA; 0.6% diet up to 84 weeks). glycogen storage (GSF) occurred mainly upon treatment NNM, amphophilic cell (APF) observed treated DHEA alone or combination NNM. The 2 types lesions belong cellular lineages, glycogenotic/basophilic lineage lineage, which are characterized by...