A5015802838- Plant biochemistry and biosynthesis
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- Plant and animal studies
- Plant Molecular Biology Research
- Plant Genetic and Mutation Studies
- Biochemical and biochemical processes
- Antioxidant Activity and Oxidative Stress
- Microbial Natural Products and Biosynthesis
- Transgenic Plants and Applications
- Essential Oils and Antimicrobial Activity
- Microtubule and mitosis dynamics
- Horticultural and Viticultural Research
- Biofuel production and bioconversion
- Natural product bioactivities and synthesis
- Plant Virus Research Studies
- Plant Physiology and Cultivation Studies
- Plant Surface Properties and Treatments
- Animal Genetics and Reproduction
- Algal biology and biofuel production
- Insect and Pesticide Research
- Plant Pathogens and Fungal Diseases
- Bee Products Chemical Analysis
Université Jean Monnet
2002-2023
Centre National de la Recherche Scientifique
2000-2023
Laboratoire de Biotechnologies Végétales Appliquées aux Plantes Aromatiques et Médicinales
2002-2023
Université Claude Bernard Lyon 1
2005-2022
Laboratoire Reproduction et Développement des Plantes
1999-2005
Institut National de la Recherche Agronomique
2005
Centre National pour la Recherche Scientifique et Technique (CNRST)
2005
École Normale Supérieure de Lyon
1996-2005
United States Department of Agriculture
2001-2004
Oklahoma State University
2004
The scent of roses (Rosa x hybrida) is composed hundreds volatile molecules. Monoterpenes represent up to 70% percent the content in some cultivars, such as Papa Meilland rose. Monoterpene biosynthesis plants relies on plastid-localized terpene synthases. Combining transcriptomic and genetic approaches, we show that Nudix hydrolase RhNUDX1, localized cytoplasm, part a pathway for free monoterpene alcohols contribute fragrance roses. RhNUDX1 protein shows geranyl diphosphate...
Sclareol is a diterpene natural product of high value for the fragrance industry. Its labdane carbon skeleton and its two hydroxyl groups also make it valued starting material semisynthesis numerous commercial substances, including production Ambrox® related ambergris substitutes used in formulation end perfumes. Most commercially-produced sclareol derived from cultivated clary sage (Salvia sclarea) extraction plant material. In sage, mainly accumulates essential oil-producing trichomes that...
Geraniol derived from essential oils of various plant species is widely used in the cosmetic and perfume industries. It also an trait pleasant smell rose flowers. In contrast to other monoterpenes which are produced plastids via methyl erythritol phosphate pathway, geraniol biosynthesis roses relies on cytosolic NUDX1 hydrolase dephosphorylates geranyl diphosphate (GPP). However, metabolic origin GPP remains unknown. By feeding Rosa chinensis "Old Blush" flowers with pathway-specific...
Male meiosis in higher organisms features synchronous cell divisions a large number of cells. It is not clear how this synchrony achieved, nor it known whether the linked to regulation cycle progression. Here, we describe an Arabidopsis mutant, named tardy asynchronous (tam), that exhibits phenotype delayed and during male meiosis. In Arabidopsis, two nuclear occur before simultaneous cytokinesis yields tetrad haploid tam, are delayed, resulting formation abnormal intermediates, most...
Nudix hydrolases are conserved enzymes ubiquitously present in all kingdoms of life. Recent research revealed that several involved terpenoid metabolism plants. In modern roses, RhNUDX1 is responsible for formation geraniol, a major compound rose scent. Nevertheless, this produced by monoterpene synthases many geraniol-producing As consequence, raised the question about origin function and NUDX1 gene evolution Rosaceae, wild roses or/and during domestication process. Here, we showed three...
In Chinese rose species and in many modern varieties, two methylated phenolic derivatives, 3,5‐dimethoxytoluene 1,3,5‐trimethoxybenzene, are major scent components. We show that cell‐free extracts of petals catalyse the synthesis 1,3,5‐trimethoxybenzene by methylation precursor molecules. An expressed sequence tag approach was used to identify four highly similar O ‐methyltransferase sequences specifically anthers. Thin layer chromatography analysis showed activities these enzymes with...
Abstract Meiosis is often described as a special case of cell division since it differs from mitosis in having two nuclear divisions without an intervening S-phase. It will be great interest to uncover what molecular mechanisms underlie these features meiosis. We previously reported that the tardy asynchronous meiosis (tam) mutant Arabidopsis (Arabidopsis thaliana) slower cycle progression male Here we report TAM encodes A-type cyclin, CYCA1;2. The point mutation tam replaced conserved...
Abstract Orcinol O-methyltransferase (OOMT) 1 and 2 catalyze the last two steps of biosynthetic pathway leading to phenolic methyl ether 3,5-dimethoxytoluene (DMT), major scent compound many rose (Rosa x hybrida) varieties. Modern roses are descended from both European Chinese species, latter being producers ethers but not former. Here we investigated why production occurs in some all In DMT-producing varieties, OOMTs were shown be localized specifically petal, predominanty adaxial epidermal...
Sclareol is a high-value natural product obtained by solid/liquid extraction of clary sage (Salvia sclarea L.) inflorescences. Because processes excretion and accumulation this labdane diterpene are unknown, the aim work was to gain knowledge on its sites in planta. Samples were collected natura or during different steps industrial process (steam distillation extraction). then analysed with combination complementary analytical techniques (gas chromatography coupled mass spectrometer,...
Roses use a non-canonical pathway involving Nudix hydrolase, RhNUDX1, to synthesize their monoterpenes, especially geraniol. Here we report the characterization of another expressed NUDX1 gene from rose cultivar Rosa x wichurana, RwNUDX1-2. In order study function RwNUDX1-2 protein, analyzed volatile profiles an F1 progeny generated by crossing R. chinensis cv. 'Old Blush' with wichurana. A correlation test volatilomes expression data revealed that is involved in biosynthesis group...
Lavandula pedunculata (Mill.) Cav. subsp. lusitanica, stoechas L. and viridis l'Hér. are three lavender taxa that belong to the botanical section Stoechas widely used as aromatherapy, culinary herb or folk medicine in many Mediterranean regions. The analysis of their bioactive volatile constituents revealed presence 124 substances, most abundant being bicyclic monoterpenes fenchone, camphor 1,8-cineole give these species respective chemotypes. Most noteworthy was fenchone which, with its...
Abstract In emb (embryo specific) mutants of maize (Zea mays), the two fertilization products have opposite fates: Although endosperm develops normally, embryo shows more or less severe aberrations in its development, resulting nonviable seed. We show here that mutant emb8516, development embryos deviates as soon transition stage from wild-type siblings. The basic events pattern formation take place because display an apical-basal polarity and differentiate a protoderm. However,...
We localized the tissues and cells that contribute to scent biosynthesis in scented non-scented Rosa x hybrida cultivars as part of a detailed cytological analysis rose petal. Adaxial petal epidermal have typical conical, papillate shape whereas abaxial are flat. Using two different techniques, solid/liquid phase extraction headspace collection volatiles, we showed that, roses, both layers capable producing emitting despite morphologies these tissues. Moreover, OOMT, an enzyme involved...
The expression of heat-shock proteins (HSPs) in isolated maize (Zea mays L.) microspores has been investigated using high-resolution two-dimensional electrophoresis coupled to immunodetection and fluorography vivo synthesized proteins. To this end, homogeneous viable populations have purified sufficient amounts for molecular analysis from plants grown controlled conditions. Appropriate conditions thermal stress application defined. revealed that display a classical response characterized by...
Male meiosis in higher organisms features synchronous cell divisions a large number of cells. It is not clear how this synchrony achieved, nor it known whether the linked to regulation cycle progression. Here, we describe an Arabidopsis mutant, named tardy asynchronous meiosis(tam), that exhibits phenotype delayed and during male meiosis. In Arabidopsis, two nuclear occur before simultaneous cytokinesis yields tetrad haploid tam, are delayed, resulting formation abnormal intermediates, most...
The rose is the queen of flowers and widely used as a garden plant for cut flower market. Roses are also production essential oil cosmetic perfume industries. A lot botanical roses scented use their volatiles to attract pollinators. Fragrances in very diverse scent has always been an important character selection process. Breeders have recently tried introduce new fragrances, instance reminiscent fruit or spice odors. But despite efforts, some on market not fragrant, especially ones selected...