A5044385434- Plant biochemistry and biosynthesis
- Wound Healing and Treatments
- Microbial Metabolic Engineering and Bioproduction
- Antioxidant Activity and Oxidative Stress
- Electrospun Nanofibers in Biomedical Applications
- Photosynthetic Processes and Mechanisms
- Antimicrobial agents and applications
- Bone Tissue Engineering Materials
- Hydrogels: synthesis, properties, applications
- Microbial Natural Products and Biosynthesis
- Endoplasmic Reticulum Stress and Disease
- Inhalation and Respiratory Drug Delivery
- Biochemical and biochemical processes
- Plant Gene Expression Analysis
- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Algal biology and biofuel production
- Graphene and Nanomaterials Applications
- Glycosylation and Glycoproteins Research
- Tissue Engineering and Regenerative Medicine
- Essential Oils and Antimicrobial Activity
- Surgical Sutures and Adhesives
- Membrane Separation Technologies
- Hemoglobin structure and function
- 3D Printing in Biomedical Research
Institute of Biocybernetics and Biomedical Engineering
2021-2024
Polish Academy of Sciences
2021-2024
Institute of Biochemistry and Biophysics, Polish Academy of Sciences
2001-2023
Institut de Chimie de Strasbourg
2019
Université de Strasbourg
2019
Centre National de la Recherche Scientifique
2019
Medical University of Warsaw
2001
Geraniol derived from essential oils of various plant species is widely used in the cosmetic and perfume industries. It also an trait pleasant smell rose flowers. In contrast to other monoterpenes which are produced plastids via methyl erythritol phosphate pathway, geraniol biosynthesis roses relies on cytosolic NUDX1 hydrolase dephosphorylates geranyl diphosphate (GPP). However, metabolic origin GPP remains unknown. By feeding Rosa chinensis "Old Blush" flowers with pathway-specific...
Isoprenoids, including dolichols (Dols) and polyprenols (Prens), are ubiquitous components of eukaryotic cells. In plant cells, there two pathways that produce precursors utilized for isoprenoid biosynthesis: the mevalonate (MVA) pathway methylerythritol phosphate (MEP) pathway. this work, contribution these to biosynthesis Prens Dols was addressed using an in planta experimental model. Treatment plants with pathway-specific inhibitors analysis effects various light conditions indicated...
The cooperation of the mevalonate (MVA) and methylerythritol phosphate (MEP) pathways, operating in parallel plants to generate isoprenoid precursors, has been studied extensively. Elucidation metabolic pathways is indispensable for rational design plant microbial systems production industrially valuable terpenoids. Here, we describe a new method, based on numerical modeling mass spectra metabolically labeled dolichols (Dols), designed quantitatively follow MVA MEP reprogrammed upon osmotic...
Arabidopsis thaliana AtNUDT7 Nudix pyrophosphatase hydrolyzes NADH and ADP-ribose in vitro is an important factor the cellular response to diverse biotic abiotic stresses. Several studies have shown that loss-of-function Atnudt7 mutant plants display many profound phenotypes. However molecular mechanism of function remains elusive. To gain a better understanding this hydrolase role, proteins interacting with were identified. Using as bait binding assay derived from cultured cell extracts we...
Purpose: The aim of the study is an approach to a nanocomposite with copper nanoparticles, constituting bacteriostatic surface for maintaining human lung cells' function. Methods: polyelectrolyte layer coating nanoparticles incorporated was designed. As factor, were applied as colloidal solution (ColloidCuNPs) and (CuNPs). influence polyelectrolytes on selected Gram(+) Gram(-) strains examined. function adenocarcinoma A549 cell line epithelial cells cultured in presence coatings analyzed,...
Mono-saturated polyprenols (dolichols) have been found in almost all Eukaryotic cells, however, dolichols containing additional saturated bonds at the ω-end, identified A. fumigatus and niger. Here we confirm using an LC-ESI-QTOF-MS analysis, that poly-saturated are abundant other filamentous fungi, Trichoderma reesei, nidulans Neurospora crassa, while yeast Saccharomyces cerevisiae only contains typical mono-saturated dolichols. We also show, differential scanning calorimetry (DSC)...
The study of the surface membrane coatings constructed with adsorbed coronavirus (COV) was described to test their suitability for antiviral activity application in personal protective and medical equipment. nanocoating based on polyethyleneimine (PEI) or polystyrene sulfonate (PSS) metallic nanoparticles incorporated investigated using AFM technique. Moreover, functioning human lung cells a configuration prepared material studied microscopic techniques flow cytometry. mean values percentage...
Euphorbia species are characterized by a net of laticifers producing large amounts triterpenes. These hydrocarbon-like metabolites can be converted into fuel the methods oil industry. lathyris is easily grown at an industrial scale. In attempt to increase its triterpene production, metabolic pathways leading isoprenoid were investigated incorporation 13C labeled glucose and mevalonate 2H deoxyxylulose as well natural abundance isotope ratio GC-MS. Latex triterpenes exclusively synthesized...
(1) Purpose: The aim of the study was to develop a nanocomposite with copper nanoparticles constituting bacteriostatic surface maintain human lung cell function. (2) Methods: A polyelectrolyte layer coating that incorporated designed. As factor, were applied as colloidal solution (ColloidCuNPs) and (CuNPs). influence polyelectrolytes on selected Gram (+) (−) strains examined. function morphology adenocarcinoma A549 line, comprising epithelial cells cultured in presence coatings, evaluated....
The study aims to approach a coating layer based on alginate functionalized with albumin nanocoated oxygenating factor, constituting safe and even contact surface epithelial cells in system components for medical devices support maintaining the human lung cells' function. incorporated albumin-coated element was designed (ALG_Alb-PFD). As an perfluorodecalin applied at three saturation levels oxygen: 0%, 21%, 100%. studies were performed adenocarcinoma A549 cell lines derived from cultured...
The ever-increasing problem of chronic, non-healing wound treatment is a challenge in contemporary medicine. study aims to verify the effect designed dressing containing coating involving oxygenating factors on promoting healing all phases single clinical case. alginate-based coating, which incorporates factor, was produced. obtained surface physicochemical features were examined using FTIR and AFM microscopy. bandage support modified with elaborated applied patients who suffered from...
The ever-increasing problem of chronic, non-healing wound treatment is a challenge in contemporary medicine. This study aims to verify the effect designed dressing containing coating involving oxygenating factors on promotion healing all phases single clinical case. An alginate-based coating, which incorporates factor, was produced. obtained surface physicochemical features were examined using FTIR and AFM. bandage support modified with developed applied patients who suffered from chronic...
Background: Wound dressing plays a vital role in post-operative aftercare. There is the necessity to develop dressings for application on border of soft and hard tissue. This study aimed multifunctional polyelectrolyte layers enhanced by hydroxyapatite nanoparticles, gold nanoparticles (AuNPs), and/or fullerenol nanocomposites achieve wound that could be applied bone-skin interface. Methods: Constructed shells were examined using TEM, STEM, EDX techniques. The human osteoblasts or...
This study is an approach to a dressing platform based on support functionalized with oxygenating factors within alginate layer, constituting safe and even contact surface for interface wound. An layer incorporated elements deposited the patch was assessed. As factor, perfluorooctyl applied, coatings in two options, cross-linked not, were evaluated. The function of human dermal fibroblast cells cultured presence these constructs analyzed, as well their morphology using flow cytometry,...
Some Trichoderma spp. exhibit natural abilities to reduce fungal diseases of plants through their mycoparasitic and antagonistic properties. In this study, we created new atroviride strains with elevated antifungal activity. This effect was achieved by improving the activity cis-prenyltransferase, main enzyme in dolichol synthesis, expressing RER2 gene from Saccharomyces cerevisiae. Since dolichyl phosphate is carrier carbohydrate residues during protein glycosylation, activation its...