A5074635677- Plant biochemistry and biosynthesis
- Photosynthetic Processes and Mechanisms
- Cellular transport and secretion
- Protist diversity and phylogeny
- Glycosylation and Glycoproteins Research
- Photoreceptor and optogenetics research
- Antioxidant Activity and Oxidative Stress
- Microtubule and mitosis dynamics
- Lipid metabolism and biosynthesis
- Plant Gene Expression Analysis
- Retinal Development and Disorders
- Natural product bioactivities and synthesis
- Microbial Metabolic Engineering and Bioproduction
- Plant Reproductive Biology
- Microbial Community Ecology and Physiology
- Receptor Mechanisms and Signaling
- Ubiquitin and proteasome pathways
- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Lipid Membrane Structure and Behavior
- Biotin and Related Studies
- Erythrocyte Function and Pathophysiology
- Retinal Diseases and Treatments
- Cancer therapeutics and mechanisms
- Plant nutrient uptake and metabolism
Institute of Biochemistry and Biophysics, Polish Academy of Sciences
2011-2023
Polish Academy of Sciences
2009-2022
Instytut Biologii Doświadczalnej im. Marcelego Nenckiego
1997-2009
Infection with avirulent pathogens, tobacco mosaic virus (TMV) or Pseudomonas syringae pv. tabaci induced accumulation of polyisoprenoid alcohols, solanesol and a family polyprenols [from polyprenol composed 14 isoprene units (Pren-14) to -18, Pren-16 dominating] in the leaves resistant plants Nicotiana tabacum cv. Samsun NN. Upon TMV infection, content was increased seven- eight-fold inoculated upper leaves, respectively, while 2.5- 2-fold on seventh day post-infection. Accumulation...
Summary Dolichol plays an indispensable role in the N ‐glycosylation of eukaryotic proteins. As proteins enter secretory pathway they are decorated by a ‘glycan’, which is preassembled onto membrane‐anchored dolichol molecule embedded within endoplasmic reticulum ( ER ). Genetic and biochemical evidence yeast animals indicate that cis ‐prenyltransferase CPT ) required for synthesis, but also point to other factor(s) could be involved. In this study, RNA i‐mediated suppression one member...
Plants accumulate a family of hydrophobic polymers known as polyprenols, yet how they are synthesized, where reside in the cell, and what role serve is largely unknown. Using Arabidopsis thaliana model, we present evidence for involvement plastidial cis-prenyltransferase (AtCPT7) polyprenol synthesis. Gene inactivation RNAi-mediated knockdown AtCPT7 eliminated leaf while its overexpression increased their content. Complementation tests polyprenol-deficient yeast ∆rer2 mutant enzyme assays...
Significance The enzyme, cis- prenyltransferase ( PTase), composed of two subunits, NgBR and DHDDS, is essential for protein glycosylation reactions in all higher eukaryotes. Here, we report the heterodimeric crystal structure complex show features that impacts stability, activity, lipid sensing enzyme complex. Moreover, rationalizes mutations cause genetic disorders leading to cognitive dysfunction, epilepsy, Parkinson’s disease.
The calcyclin-binding protein (CacyBP) binds calcyclin (S100A6) at physiological levels of [Ca(2+)] and is highly expressed in brain neurons. Subcellular localization CacyBP was examined neurons neuroblastoma NB-2a cells different [Ca(2+)](i). Immunostaining indicates that present the cytoplasm unstimulated cultured which resting [Ca(2+)](i) known to be approximately 50 nm. When increased above 300 nm by KCl treatment, immunostaining mainly apparent as a ring around nucleus. Such perinuclear...
Dolichol is a required cofactor for protein glycosylation, the most common posttranslational modification modulating stability and biological activity of proteins in all eukaryotic cells. We have identified characterized two genes, PPRD1 -2, which are orthologous to human SRD5A3 (steroid 5α reductase type 3) encode polyprenol reductases responsible conversion dolichol Arabidopsis thaliana. -2 play dedicated roles plant metabolism. PPRD2 essential viability; its deficiency results aberrant...
Dehydrodolichyl diphosphate synthase (DHDDS) catalyzes the committed step in dolichol synthesis. Recessive mutations DHDDS cause retinitis pigmentosa (RP59), resulting blindness. We hypothesized that rod photoreceptor-specific ablation of Dhdds would retinal degeneration due to diminished dolichol-dependent protein N-glycosylation. Dhddsflx/flx mice were crossed with rod-specific Cre recombinase-expressing (Rho-iCre75) generate knockout (Dhddsflx/flx iCre+). In vivo morphological and...
The cooperation of the mevalonate (MVA) and methylerythritol phosphate (MEP) pathways, operating in parallel plants to generate isoprenoid precursors, has been studied extensively. Elucidation metabolic pathways is indispensable for rational design plant microbial systems production industrially valuable terpenoids. Here, we describe a new method, based on numerical modeling mass spectra metabolically labeled dolichols (Dols), designed quantitatively follow MVA MEP reprogrammed upon osmotic...
The widespread occurrence of polyprenols throughout the plant kingdom is well documented, yet their functional role poorly understood. These lipophilic compounds are known to be assembled from isoprenoid precursors by a class enzymes designated as cis-prenyltransferases (CPTs), which encoded small CPT gene families in plants. In this study, we report that RNA interference (RNAi)-mediated knockdown one member tomato family (SlCPT5) reduced leaves about 70%. Assays with recombinant SlCPT5...
Arabidopsis roots accumulate a complex mixture of dolichols composed three families, (i.e., short-, medium- and long-chain dolichols), but until now none the cis-prenyltransferases (CPTs) predicted in genome has been considered responsible for their synthesis. In this report, using homo- heterologous (yeast tobacco) models, we have characterized AtCPT1 gene (At2g23410) which encodes CPT formation dolichols, Dol-18 to -23, with Dol-21 dominating, Arabidopsis. The content these was...
Brassinosteroids are important plant hormones influencing, among other processes, chloroplast development, the electron transport chain during light reactions of photosynthesis, and Calvin-Benson cycle. Medium-chain-length polyprenols built 9–11 isoprenoid units (C45–C55 carbons) a class compounds present in abundance thylakoid membranes. They synthetized by CPT7 gene from Calvin cycle derived precursors on MEP (methylerythritol 4-phosphate) biosynthesis pathway. C45–C55 affect membrane...
Evidence that dynamin is associated with the sequestration of Paramecium beta(2)-adrenergic receptor (betaAR) immunoanalogue presented. We previously reported a dramatic change in distribution betaAR analogue subcellular fractions upon isoproterenol treatment: it redistributed from membraneous to cytosolic fraction, as revealed by quantitative image analysis western blots. Here we confirm and extend this observation laser scanning confocal immunogold electron microscopy. In presence (10...
Dolichols (Dols), ubiquitous components of living organisms, are indispensable for cell survival. In plants, as well other eukaryotes, Dols crucial post-translational protein glycosylation, aberration which leads to fatal metabolic disorders in humans and male sterility plants. Until now, the mechanisms underlying Dol accumulation remain elusive. this study, we have analysed natural variation six isoprenoids among more than 120 Arabidopsis thaliana accessions. Subsequently, by combining QTL...
The first evidence of dynamin presence and its colocalization with clathrin in the compartment involved Paramecium receptor-mediated endocytosis is presented. We identified by cloning, Western blotting, immunodetection confocal electron microscopy. partial genes, which we have designated ParDyn1 ParDyn2, are 1091 bp long, 90% identical to one another encode N-terminal middle domains isoform 1 2. deduced amino acid sequences contain all three guanosine 5′-triphosphate (GTP)-binding motifs...
Rab7 is a small GTPase that plays crucial role in the regulation of transport from early to late endosomes and lysosomes, phagosome maturation lysosomal biogenesis mammalian cells. It contains conserved unique sequence elements mediate its function. Two genes, Rab7a (703 bp) Rab7b (707 were identified unicellular eukaryote Paramecium by PCR amplification. They contain three short introns different lengths (28-32 located at identical positions both genes. The presence two genes genome was...
Lipid anchors are common post-translational modifications for proteins engaged in signaling and vesicular transport eukaryotic cells. Rab geranylgeranylated at their C-termini, a modification which is important stable binding to lipid bilayers. The escort protein (REP) an accessory of the geranylgeranyl transferase (RGT) complex it obligatory prenylation. While REP-Rab interactions have been studied by biochemical, structural, genetic methods animals yeast, data on plant RGT still limited....
The kinetics of the uptake fluid phase marker Lucifer Yellow (LY), and its alteration by wortmannin, an inhibitor phosphatidylinositol-3 kinase (PI-3K), PKC modulators: GF 109203 X, inhibitor, phorbol ester, activator was studied in eukaryotic model Paramecium aurelia. Spectrophotometric quantification LY accumulation performed presence or absence transferrin, a receptor-mediated endocytosis. Internalization showed curvilinear kinetics: high initial rate LYuptake (575 ng LY/ mg protein /hr)...