A5071742126- Alkaloids: synthesis and pharmacology
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Berberine and alkaloids research
- Traditional and Medicinal Uses of Annonaceae
- Nanoparticles: synthesis and applications
- Parasite Biology and Host Interactions
- Reproductive biology and impacts on aquatic species
- Plant biochemistry and biosynthesis
- Insects and Parasite Interactions
- Herbal Medicine Research Studies
- Nanoparticle-Based Drug Delivery
- Helminth infection and control
- Advanced Nanomaterials in Catalysis
- Aquaculture disease management and microbiota
- Mollusks and Parasites Studies
- Plant tissue culture and regeneration
- Wound Healing and Treatments
- Botanical Research and Applications
- Melanoma and MAPK Pathways
- Marine and coastal ecosystems
- Marine Biology and Environmental Chemistry
- Medicinal Plant Pharmacodynamics Research
- Immunotherapy and Immune Responses
University of Warsaw
2014-2025
The interaction between silver nanoparticles and herpesviruses is attracting great interest due to their antiviral activity possibility use as microbicides for oral anogenital herpes. In this work, we demonstrate that tannic acid modified sized 13 nm, 33 nm 46 are capable of reducing HSV-2 infectivity both in vitro vivo. was size-related, required direct blocked virus attachment, penetration further spread. All tested reduced infection inflammatory reaction the mouse model when used at or a...
Studying the role of nanoparticles in plant cryopreservation is essential for developing innovative methods to conserve genetic resources amid environmental challenges. This research investigated effects gold (AuNPs), silver (AgNPs), and zinc oxide (ZnONPs) on structural integrity, stability, metabolic activity cryopreserved materials with medicinal properties. Shoot tips from two bleeding heart (Lamprocapnos spectabilis (L). Fukuhara) cultivars, 'Gold Heart' 'Valentine', were using...
Bacteriophages have emerged as promising natural antibacterial agents, offering a targeted approach to combating bacterial infections. While phage-antibiotic cocktails are widely explored enhance efficacy and prevent resistance, research on phage-nanoparticle combinations remains limited. However, antibiotic resistance continues rise, necessitating alternative strategies. Combining bacteriophages with nanoparticles presents novel that could potency while reducing the risk of yet studies in...
Most studies on the effects of organisms microplastic characteristics have focused microorganisms, while impact animal feeding behavior, particularly in aquatic species like fish and decapod crustaceans, has been less explored. This study examines how polyethylene spherical microplastics (275 μm diameter) passing through digestive tracts crucian carp (Carassius carassius) Australian crayfish (Cherax quadricarinatus) affect surface properties, particle size, bacterial colonization. The were...
Abstract Transport protein particle (TRAPP) tethering complexes are known for their function as Rab GTPase exchange factors. Two versions of the complex considered functionally separate: TRAPPII, an activator Rab11 family (RabA in plants) GTPases that post-Golgi sorting, and TRAPPIII, activating Rab1 (RabD members regulate endoplasmic reticulum (ER)-to-Golgi trafficking autophagy. In Arabidopsis (Arabidopsis thaliana), TRAPPIII has been identified its subunit composition established, but...
Silver nanoparticles (AgNPs) are promising new antimicrobial agents against a wide range of skin and mucosal pathogens. However, their interaction with the immune system is currently not fully understood. Dendritic cells (DCs) crucial during development T cell-specific responses bacterial viral We have previously shown that tannic acid-modified silver (TA-AgNPs) consist microbicide HSV-2. The aim this study was to compare ability TA-AgNPs or TA-AuNPs similar sizes (TA-Ag/AuNPs) induce DCs...
Iron( iii ) oxide nanoparticles doped with magnesium exposed to an alternating magnetic field induced cytotoxic effects on lung cancer cells (A549).
Aim: To investigate the in vitro activity of silver NPs (AgNPs) against pathogenic microalgae Prototheca genus. Materials & methods: The antialgal potential AgNPs species both clinical and environmental origin was assessed from minimum inhibitory (algistatic) algicidal concentrations. cytotoxicity bovine mammary epithelial cell line evaluated by means standard MTT assay.Results: showed a strong killing toward algae, as minimal concentration (MAC) values matched perfectly corresponding (MIC)...
Dolichol is a required cofactor for protein glycosylation, the most common posttranslational modification modulating stability and biological activity of proteins in all eukaryotic cells. We have identified characterized two genes, PPRD1 -2, which are orthologous to human SRD5A3 (steroid 5α reductase type 3) encode polyprenol reductases responsible conversion dolichol Arabidopsis thaliana. -2 play dedicated roles plant metabolism. PPRD2 essential viability; its deficiency results aberrant...
Prototheca species are unicellular, nonphotosynthetic, saprophytic, and occasionally pathogenic, microalgae, with an extensive environmental reservoir. This study explores, for the first time, occurrence of in aquatic ecosystems by using a molecular profiling approach. A total 362 samples were collected from 80 natural artificial waterbodies at 88 sampling sites 26 localities across Poland during 1.5-year period. The overall isolation rate water environments was 14.1%. most prevalent rivers...
Rab proteins, key players in vesicular transport all eukaryotic cells, are post-translationally modified by lipid moieties. Two geranylgeranyl groups attached to the protein heterodimeric enzyme transferase (RGT) αβ. Partial impairment this activity Arabidopsis, disruption of AtRGTB1 gene, is known influence plant stature and disturb gravitropic light responses. Here it shown that mutations each RGTB genes cause a tip growth defect, visible as root hair pollen tube deformations. Moreover, FM...
Eukaryotic cells rely on the accuracy and efficiency of vesicular traffic. In plants, disturbances in trafficking are well studied quickly dividing root meristem or polar growing hairs pollen tubes. The development female gametophyte, a unique haploid reproductive structure located ovule, has received far less attention studies transport. Key molecules providing specificity vesicle formation its subsequent recognition fusion with acceptor membrane Rab proteins. Rabs anchored to membranes by...
Our study aimed at examining the phylogenetic position of newly-found Setaria nematodes obtained from red deer (Cervus elaphus) based on sequences mitochondrial cytochrome c oxidase subunit 1 (COX-1). Alignment and analyses, as well SEM microscopic analysis, revealed presence two species: S. cervi tundra. tundra was noted in only one individual, a calf deer, while observed three stages, hinds deer. According to our knowledge, it is first case Poland confirmed molecular studies also infection
Micro- (MPs) and nanoplastics (NPs) are currently ubiquitous in the ecosystems, freshwater biota is still insufficiently studied to understand global fate, transport paths, consequences of their presence. Thus, this study, we investigated role bivalves a trophic transfer MPs NPs an experimental food chain. The chain consisted terrestrial non-selective detritivore Dendrobaena (Eisenia) sp., benthic filter feeder Unio tumidus, detritivore-collectors Asellus aquaticus or Gammarus sp. Animals...
Histological and histochemical features of the oesophagogastric segment alimentary canal as well ultrastructure gastric gland cells freshwater tubenose goby Proterorhinus semilunaris were examined. The studies revealed that despite lack anatomical distinction, is histologically divided into oesophagus, oesogaster stomach, which provides evidence for functional compartmentation this organ. oesophagus was characterised by presence numerous goblet secreting mainly a mixture neutral acid...
Although the Dictyocaulus lungworm, agent of dictyocaulosis, is one parasitological threats to European bison, its systematic position remains unclear. The aim present study was evaluate morphological features lungworm and pathological lesions it induces, analyse mitochondrial (mt) genetic markers for molecular epidemiological studies. findings indicate that lungworms bison can be distinguished from those cattle on basis differences in buccal capsule wall length, total body spicules length...
There is an ongoing search for alternative treatments Clostridioides difficile infections. The aim of the study was to investigate antibacterial and antibiotic activity bee products against C. strains with different polymerase chain reaction ribotypes (RTs). minimum inhibitory concentration (MICs) Manuka honey 550+, goldenrod honey, pine bread were determined by broth dilution method. adhesion HT-29, HT-29 MTX, CCD 841 CoN cell lines assessed. Biofilm cultured in titration plates visualized...
Brassinosteroids are important plant hormones influencing, among other processes, chloroplast development, the electron transport chain during light reactions of photosynthesis, and Calvin-Benson cycle. Medium-chain-length polyprenols built 9–11 isoprenoid units (C45–C55 carbons) a class compounds present in abundance thylakoid membranes. They synthetized by CPT7 gene from Calvin cycle derived precursors on MEP (methylerythritol 4-phosphate) biosynthesis pathway. C45–C55 affect membrane...
Tetraspanin CD9 is the only protein of oocyte membrane (oolemma) known to be required for fusion gametes during fertilization in mouse. Using electron microscopy and immunostaining we examined differences localization between ovulated oocytes, zygotes parthenogenetically activated eggs (parthenogenotes). Changes ultrastructure oolemma, which take place oocytes after or artificial activation, were also assessed. We demonstrated that level present on microvilli zygote was two times lower than...
Lungworms of the genus Dictyocaulus are causative agents parasitic bronchitis in domestic and wild ungulates. This study investigates distribution, morphology genetic diversity D. cervi a new lungworm species, skrjabini n. sp. infecting red deer Cervus elaphus, fallow Dama dama moose Alces alces Poland Sweden. The was conducted on 167 from DNA lungworms derived 7 deer, 4 2 collected prevalence dissected 31.1% 7.2%, respectively. Moreover, confirmed molecularly isolates 1 isolate established...