A5043252686- Air Quality and Health Impacts
- Energy and Environment Impacts
- Nanoparticles: synthesis and applications
- Climate Change and Health Impacts
- Air Quality Monitoring and Forecasting
- Microplastics and Plastic Pollution
- Carcinogens and Genotoxicity Assessment
- Vehicle emissions and performance
- Research Data Management Practices
- Atmospheric chemistry and aerosols
- Epigenetics and DNA Methylation
- Chemistry and Chemical Engineering
- COVID-19 impact on air quality
- Advanced biosensing and bioanalysis techniques
- Graphene and Nanomaterials Applications
- Mineral Processing and Grinding
- 3D Printing in Biomedical Research
- Animal testing and alternatives
- Geochemistry and Geologic Mapping
- Histone Deacetylase Inhibitors Research
- Indoor Air Quality and Microbial Exposure
- Scientific Computing and Data Management
- Energy, Environment, and Transportation Policies
- Glutathione Transferases and Polymorphisms
- Big Data and Business Intelligence
NILU
2020-2024
University of Milano-Bicocca
2010-2019
University of Milan
2014
This study explores and characterizes cell cycle alterations induced by urban PM2.5 in the human epithelial line BEAS-2B, elucidates possible mechanisms involved. The cells were exposed to a low dose (7.5 μg/cm2) of Milan winter for different time points, progression was analyzed fluorescent microscopy flow cytometry. Activation proteins involved control investigated Western blotting DNA damage 32P-postlabelling, immunostaining comet assay. formation reactive oxygen species (ROS) quantified...
The Alamar Blue (AB) assay is widely used to investigate cytotoxicity, cell proliferation and cellular metabolic activity within different fields of toxicology. use the with nanomaterials (NMs) entails specific aspects including potential interference NMs test. procedure AB applied for testing described in detail step-by-step, from NM preparation, exposure, inclusion controls, analysis interpretation results. Provided that proper followed, relevant controls are included, a reliable high...
Emissions from diesel vehicles and biomass burning are the principal sources of primary ultrafine particles (UFP). The exposure to UFP has been associated cardiovascular pulmonary diseases, including lung cancer. Although many aspects toxicology ambient particulate matter (PM) have unraveled, molecular mechanisms activated in human cells by still poorly understood. Here, we present an RNA-seq time-course experiment (five time point after single dose exposure) used investigate differential...
Acquired drug resistance and metastasis in breast cancer (BC) are coupled with epigenetic deregulation of gene expression. Epigenetic drugs, aiming to reverse these aberrant transcriptional patterns sensitize cells other therapies, provide a new treatment strategy for drug-resistant tumors. Here we investigated the ability DNA methyltransferase (DNMT) inhibitor decitabine (DAC) increase sensitivity BC anthracycline antibiotic doxorubicin (DOX). Three cell lines representing different...
Abstract Genotoxicity testing for nanomaterials remains challenging as standard approaches require some adaptation, and further development of nano-specific OECD Test Guidelines (TGs) Guidance Documents (GDs) are needed. However, the field genotoxicology continues to progress new approach methodologies (NAMs) being developed that could provide relevant information on range mechanisms genotoxic action may be imparted by nanomaterials. There is a recognition need implementation and/or adapted...
Particulate matter (PM) exposure is related to pulmonary and cardiovascular diseases, with increased inflammatory status. The release of the proinflammatory interleukin- (IL-) 1β, controlled by a dual pathway, formation inactive pro-IL-1β, through Toll-like receptors (TLRs) activation, its cleavage NLRP3 inflammasome. THP-1-derived macrophages were exposed for 6 h 2.5 μg/cm(2) Milan PM10, potential promote IL-1β binding TLRs activating has been examined. Summer induced marked response in...
Summary This study aims to critically evaluate the effect of photochemical smog on COPD patients in Nigeria. A cohort was recruited from two large hospitals Abuja Symptom questionnaires, daily diaries, dyspnoea scale and measurement lung function were used obtain health data. Real-time air pollution data obtained a monitoring site at airport. Measured concentrations, for precursors smog, are far higher than those recorded developed world where link between respiratory has been observed....
Management of nanomaterials and nanosafety data needs to operate under the FAIR (findability, accessibility, interoperability, reusability) principles this requires a unique, global identifier for each nanomaterial. Existing identifiers may not always be applicable or sufficient definitively identify specific nanomaterial used in particular study, resulting use textual descriptions research project communications reporting. To ensure that internal documentation can later linked publicly...