A5055831863- Cancer Cells and Metastasis
- Mesenchymal stem cell research
- Virus-based gene therapy research
- Cancer Research and Treatments
- Testicular diseases and treatments
- Cancer, Hypoxia, and Metabolism
- Immune Cell Function and Interaction
- RNA Interference and Gene Delivery
- Cancer Genomics and Diagnostics
- CAR-T cell therapy research
- Sarcoma Diagnosis and Treatment
- Melanoma and MAPK Pathways
- Histone Deacetylase Inhibitors Research
- Epigenetics and DNA Methylation
- Immunotherapy and Immune Responses
- Animal Disease Management and Epidemiology
- X-ray Diffraction in Crystallography
- Vector-Borne Animal Diseases
- Crystallization and Solubility Studies
- Cancer Diagnosis and Treatment
- Wnt/β-catenin signaling in development and cancer
- T-cell and Retrovirus Studies
- Cell death mechanisms and regulation
- Chemokine receptors and signaling
- Cancer Mechanisms and Therapy
Biomedical Research Center of the Slovak Academy of Sciences
2016-2025
Cancer Research Institute of the Slovak Academy of Sciences
2015-2025
Comenius University Bratislava
2023-2025
Slovak Academy of Sciences
2012-2024
National Institute of Cardiovascular Diseases
2023
Molecular Oncology (United States)
2014-2021
University Hospital Brno
2018
Masaryk University
2018
Max Perutz Labs
2005-2007
Vienna Biocenter
2005-2007
Abstract Human adipose tissue–derived mesenchymal stem cells (AT-MSC) are considered to be a promising source of autologous in personalized cell-based therapies. Tumor tracking properties MSC provide an attractive opportunity for targeted transgene delivery into the sites tumor formation. In present study, we addressed whether suicide gene introduction human AT-MSC could produce tumor-specific prodrug converting cellular vehicle chemotherapy. We prepared yeast fusion cytosine...
Human mesenchymal stromal cells (MSC) hold a promise for future cell-based therapies due to their immunomodulatory properties and/or secretory activity. Nevertheless non-neoplastic tumor compartment could also originate from MSC. We aimed show whether multipotent MSC derived human adipose tissue (AT-MSC) create cell-protective milieu and affect cell behaviour in vitro vivo. Here we have demonstrated tumor-promoting effect of AT-MSC on melanoma A375 cells. coinjection mediated abrogation...
The ability of human adipose tissue–derived mesenchymal stem cells (AT-MSCs), engineered to express the suicide gene cytosine deaminase::uracil phosphoribosyltransferase (CD::UPRT), convert relatively nontoxic 5-fluorocytosine (5-FC) into highly toxic antitumor 5-fluorouracil (5-FU) together with their track and engraft tumors micrometastases makes these an attractive tool activate prodrugs directly within tumor mass. In this study, we tested feasibility efficacy therapeutic function as...
Abstract Background Previously, we validated capability of human adipose tissue‐derived mesenchymal stem cells (AT‐MSC) to serve as cellular vehicles for gene‐directed enzyme prodrug molecular chemotherapy. Yeast fusion cytosine deaminase : uracil phosphoribosyltransferase expressing AT‐MSC (CD y ‐AT‐MSC) combined with systemic 5‐fluorocytosine (5FC) significantly inhibited growth colon cancer xenografts. We aimed determine the cytotoxic efficiency other tumour both in vitro and vivo ....
Mesenchymal stromal cells (MSCs) represent heterogeneous cell population suitable for therapies in regenerative medicine. MSCs can also substantially affect tumor biology due to their ability be recruited the stroma and interact with malignant via direct contacts paracrine signaling. The aim of our study was characterize molecular changes dictated by adipose tissue-derived mesenchymal (AT-MSCs) effects on drug responses human breast cancer SKBR3. were either directly cocultured AT-MSCs or...
Cells of the tumor microenvironment are recognized as important determinants biology. The adjacent non-malignant cells can regulate drug responses cancer by secreted paracrine factors and direct interactions with cells. Human mesenchymal stromal (MSC) actively contribute to microenvironment. Here we focused on their response chemotherapy during treatment these become affected. We have shown that secretory phenotype behavior influenced cisplatin differs from naïve MSC. MSC were more resistant...
Recent evidence in cancer research, developed the notion that malignant tumors consist of different subpopulations cells, one them, known as stem being attributed many important properties such enhanced tumorigenicity, proliferation potential and profound multidrug resistance to chemotherapy. Several key cells markers were identified colon cancer. In our study we focused on aldehyde dehydrogenase type 1 (ALDH1) expression cancer-derived cell lines HT-29/eGFP, HCT-116/eGFP LS-180/eGFP, its...
Efficiency of colorectal carcinoma treatment by chemotherapy is diminished as the resistance develops over time in patients. The same holds true for 5-fluorouracil, drug used first line carcinoma.Chemoresistant derivative HT-29 cells was prepared long-term culturing increasing concentration 5-fluorouracil. Cells were characterized viability assays, flow cytometry, gene expression arrays and kinetic imaging. Immunomagnetic separation isolation subpopulations positive cancer stem cells-related...
Cisplatin resistance in testicular germ cell tumors (TGCTs) is a clinical challenge. We investigated the underlying mechanisms associated with cancer stem (CSC) markers and modalities circumventing chemoresistance. Chemoresistant models (designated as CisR) of human embryonal carcinoma lines NTERA-2 NCCIT were derived characterized using flow cytometry, gene expression, functional protein arrays. Tumorigenicity was determined on immunodeficient mouse model. Disulfiram used to examine...
Acquired drug resistance and metastasis in breast cancer (BC) are coupled with epigenetic deregulation of gene expression. Epigenetic drugs, aiming to reverse these aberrant transcriptional patterns sensitize cells other therapies, provide a new treatment strategy for drug-resistant tumors. Here we investigated the ability DNA methyltransferase (DNMT) inhibitor decitabine (DAC) increase sensitivity BC anthracycline antibiotic doxorubicin (DOX). Three cell lines representing different...
Patients with treatment-refractory/relapsing germ cell tumors (GCTs) have a dismal prognosis due to lack of any effective therapy. Moreover, the efficacy newly approved targeted therapies remains unexplored for cisplatin-resistant GCTs. Previously, it was demonstrated that folate receptor α (FRα) is overexpressed in many tumor types and efficiently by antibody-drug conjugate (ADC) mirvetuximab soravtansine (MIRV) cancers. We hypothesized FRα represents an attractive target treating...
Ablation of the Raf-1 protein causes fetal liver apoptosis, embryonic lethality, and selective hypersensitivity to Fas-induced cell death. Furthermore, Raf-1–deficient cells show defective migration as a result deregulation Rho effector kinase Rok-α. In this study, we that kinase-independent modulation Rok-α signaling is also basis antiapoptotic function Raf-1. Fas activation stimulates formation Raf-1–Rok-α complexes, up-regulated in cells. This leads increased clustering membrane...
RNF43 is an E3 ubiquitin ligase and known negative regulator of WNT/β-catenin signaling. We demonstrate that also a noncanonical WNT5A-induced signaling in human cells. Analysis the interactome using BioID immunoprecipitation showed can interact with core receptor complex components dedicated to Wnt pathway such as ROR1, ROR2, VANGL1, VANGL2. triggers VANGL2 ubiquitination proteasomal degradation clathrin-dependent internalization ROR1 inhibits ROR2 activation. These activities are...
Epithelial-mesenchymal interactions are important not only to direct the course of prenatal development skin and its appendages but also influence behaviour transformed epithelial cells.Evaluation role stromal fibroblasts on phenotype cells basal cell carcinoma (BCC).The human BCC was compared with in vitro model where growth phenotypic pattern normal keratinocytes were monitored co-culture prepared from stroma (BCCFs), dermal or two established fibroblast lines. We visualized expression a...
Human adipose tissue was shown to be a very attractive source of mesenchymal stromal cells that have wide scale potential applications in reconstructive plastic surgery and regenerative medicine. However, these were described profound effects on biological behaviour tumour cells. The aim this study analyze the influence tissue-derived human (AT-MSC) proliferation breast cancer We tested three different cell lines under AT-MSC derived soluble factors as well direct cocultures. These data...