A5087571664- Acute Lymphoblastic Leukemia research
- Hematopoietic Stem Cell Transplantation
- Autophagy in Disease and Therapy
- Immune Cell Function and Interaction
- Cell death mechanisms and regulation
- Acute Myeloid Leukemia Research
- Heat shock proteins research
- Neuroblastoma Research and Treatments
- Melanoma and MAPK Pathways
- NF-κB Signaling Pathways
- Childhood Cancer Survivors' Quality of Life
- RNA Interference and Gene Delivery
- Hippo pathway signaling and YAP/TAZ
- Lung Cancer Research Studies
- Plant responses to water stress
- Erythrocyte Function and Pathophysiology
- Toxin Mechanisms and Immunotoxins
- Chronic Myeloid Leukemia Treatments
- Mesenchymal stem cell research
St Anna Children's Hospital
2008-2017
Vienna Biocenter
2002
Czech Academy of Sciences, Institute of Microbiology
2001
Single-laboratory experience showed that flow cytometric (FCM) assessment of minimal residual disease (MRD) in acute lymphoblastic leukemia (ALL) is feasible most patients and gives independent prognostic information. It is, however, not known whether FCM analysis can reliably be standardized for multicentric application.An extensive standardization program was installed four collaborating laboratories, which study FCM-MRD children treated with the AIEOP-BFM-ALL 2000 protocol. This included...
Changes of antigen expression on residual blast cells acute lymphoblastic leukemia (ALL) occur during induction treatment. Many markers used for phenotyping and minimal disease (MRD) monitoring are affected. Glucocorticoid (GC)-induced modulation has been causally suspected, however, subclone selection may also cause the phenomenon.We investigated this by following phenotypic evolution leukemic with flow cytometry from diagnosis to four time points after GC containing chemotherapy in 20 (of...
The Raf kinases are key signal transducers activated by mitogens or oncogenes. best studied isoform, Raf-1, was identified as an inhibitor of apoptosis conventional and conditional gene ablation in mice. c-raf-1−/− embryos growth retarded anemic, die at midgestation with anomalies the placenta fetal liver. Here, we show that Raf-1–deficient primary erythroblasts cannot be expanded culture due to their accelerated differentiation into mature erythrocytes. In addition, Raf-1 expression is...
Abstract Background: Immunophenotypic modulation is induced by steroids in pediatric B‐cell precursor acute lymphoblastic leukemia (BCP‐ALL) patients during remission induction therapy. Methods: We cultured BCP‐ALL blasts from diagnostic bone marrow (BM) samples ( n = 20) the presence of prednisone on stroma layer obtained BM‐derived mesenchymal cells to maintain viability. Antigen expression was assessed multiparametric flow cytometry. Results: Leukemia that sustained treatment vitro with...
Abstract Results suggest that CD99 may play a physiologic role in the clonal deletion processes necessary for B-lymphoid selection. Our study was performed to examine of normal and leukemia BCPs. is strongly expressed by certain pediatric cancers including BCP-ALL. Modulation antigen ETs T cells induces apoptosis, hence implicating as potential target anti-cancer therapy. However, nothing known about these aspects We investigated BCP-ALL cases BCP from BM protein RNA expression well effects...
CD99 is present in many human cell types, including high-level surface expression on pediatric B and T leukemias Ewing tumors (ETs). On lymphocytes respective malignancies, its level decreases with the stage of maturation. Inter-individual variability B-cell precursor acute lymphoblastic leukemia (BCP-ALL) blasts was shown recently to be associated distinct cytogenetic backgrounds. However, targets remain mainly unknown. Here, we show that administration an anti-CD99 antibody B- T-leukemia...
Bone marrow-derived mesenchymal stromal cells (MSCs) are multipotent with a high constitutive level of autophagy and low expression CD99. Under certain conditions, MSCs may develop tumorigenic properties. However, these transformation-induced conditions largely unknown. Recently, we have identified an association between Hsp70, main participant in cellular stress response tumorigenesis, Preliminary observations had revealed upregulation both proteins stressed long-term cultured MSCs. And so...